Crude Enzymatic Extract
Mostrando 1-12 de 58 artigos, teses e dissertações.
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1. FATTY ACID ETHYL ESTERS FROM MICROALGAE OF Scenedesmus ecornis BY ENZYMATIC AND ACID CATALYSIS
Microalgae are an indispensable food source for the various growth stages of mollusks, crustaceans, and several fish species. Using a microalgae biomass present in the Amazonian ecosystem (Macapá-AP), we study extraction methods for fatty acid such as solvent extraction (magnetic stirring and/or Soxhlet) and/or hydrolysis (acid and/or enzymatic catalysis) f
Quím. Nova. Publicado em: 2018-01
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2. Keratinolytic abilities of
Micrococcus luteus from poultry wasteKeratinolytic microorganisms have become the subject of scientific interest due to their ability to biosynthesize specific keratinases and their prospective application in keratinic waste management. Among several bacterial classes, actinobacteria remain one of the most important sources of keratin-degrading strains, however members of the
Microco Braz. J. Microbiol.. Publicado em: 21/07/2015
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3. Peptidase with Keratinolytic Activity Secreted by Aspergillus terreus During Solid-State Fermentation
The aim of this study was to evaluate peptidase production by Aspergillus terreus in solid-state bioprocess and evaluate its parameters. The best conditions were 5.0 g of wheat bran as substrate, incubation temperature 30°C, inoculum 2.0x105spores/g and 75% saline volume, with production reaching 677 U/mL (5400 U/g culture medium) after 72 h of fermentation
Braz. arch. biol. technol.. Publicado em: 2014-08
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4. Concentration, characterization and application of lipases from Sporidiobolus pararoseus strain
Lipases produced by a newly isolated Sporidiobolus pararoseus strain have potential catalytic ability for esterification reactions. After production, the enzymatic extracts (conventional crude and precipitated, 'CC' and 'CP', and industrial crude and precipitated, 'IC' e 'IP') were partially characterized. The enzymes presented, in general, higher specificit
Braz. J. Microbiol.. Publicado em: 2014
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5. Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis
The aim of this study was to determine the activity of cytokinin dehydrogenase (CKX) and to measure other biochemical components in the primary leaves and radicles of castor seedlings (BRS Energia) in the initial phase of growth. The crude protein extract obtained after a 1-h extraction from the root tissues of seedlings showed no detectable CKX enzymatic ac
Braz. arch. biol. technol.. Publicado em: 08/11/2013
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6. Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum sp
Epicoccum sp. showed in vitro antagonism to the rice pathogens Magnaporthe oryzae, Rhizoctonia solani, Sarocladium oryzae, Monographella albescens and Cochliobolus miyabeanus in dual culture. The colony growth of the rice blast fungus, M. oryzae, was reduced by 42.50%. The lethal doses (LD50) determined based on probit-log dosage response curves at 3 and 6 h
Trop. plant pathol.. Publicado em: 27/08/2013
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7. Isolation of a yeast strain able to produce a polygalacturonase with maceration activity of cassava roots
The objective of the present study was the isolation of a yeast strain, from citrus fruit peels, able to produce a polygalacturonase by submerged fermentation with maceration activity of raw cassava roots. Among 160 yeast strains isolated from citrus peels, one strain exhibited the strongest pectinolytic activity. This yeast was identified as Wickerhamomyces
Food Sci. Technol. Publicado em: 24/05/2013
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8. Produção de concentrados de ácidos graxos por hidrólise de óleos vegetais mediada por lipase vegetal
The aim of this work was to verify the ability of enzymatic crude extract from dormant castor bean seeds to yield concentrated fatty acids by hydrolysis of polyunsaturated vegetable oils such as corn and sunflower. The enzymatic extract exhibited higher activity towards corn oil, which was selected for further studies to determine optimum hydrolysis conditio
Quím. Nova. Publicado em: 2013
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9. Atividade de extratos de Arrabidaea chica (Humb. & Bonpl.) Verlot obtidos por processos biotecnológicos sobre a proliferação de fibroblastos e células tumorais humanas
Arrabidaea chica (H&B) Verlot is a plant popularly known as Pariri and this species is a known source of anthocyanins, flavonoids and tannins. This report describes an approach involving enzymatic treatment prior to extraction procedures to enhance A chica crude extract anticancer activity. Anticancer activity in human cancer cell lines in vitro using a 48 h
Quím. Nova. Publicado em: 2013
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10. Obtenção de inoculante e de coquetel enzimático lignocelulolítico a partir de comunidades microbianas termofílicas / Acquisition of an inoculant and a lignocellulolytic enzymatic cocktail from thermophilic microbial communities
Three thermophilic lignocellulolytic microbial communities were selected by enriched method with continual subcultivation at 55 C. One community was selected from cow manure compost, another from decaying sugar cane bagasse, and the last one was obtained by mixing aliquots of the culture suspension from the first two. Those communities were able to degrade a
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 17/02/2012
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11. The hydrolysis of agro-industrial residues by holocellulose-degrading enzymes
Holocellulose structures from agro-industrial residues rely on main and side chain attacking enzymes with different specificities for complete hydrolysis. Combinations of crude enzymatic extracts from different fungal species, including Aspergillus terreus, Aspergillus oryzae, Aspergillus niger and Trichoderma longibrachiatum, were applied to sugar cane baga
Brazilian Journal of Microbiology. Publicado em: 2012-06
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12. Distinct chitinases are expressed during various growth phases of the human pathogen Paracoccidioides brasiliensis
The aim of this work was the partial purification and subsequent evaluation of chitinase expression during the various growth phases of Paracoccidioides brasiliensis. Initially, PbCTS1r was expressed as a recombinant protein and displayed enzymatic activity against 4-MU-[N-acetylglucosamine (GlcNAc)]3 and 4-MU-(GlcNAc)2. Two proteins, 45 kDa and 39 kDa in si
Mem. Inst. Oswaldo Cruz. Publicado em: 2012-05