Dna Dosimetry
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Low-intensity red and infrared laser effects at high fluences on Escherichia coli cultures
Semiconductor laser devices are readily available and practical radiation sources providing wavelength tenability and high monochromaticity. Low-intensity red and near-infrared lasers are considered safe for use in clinical applications. However, adverse effects can occur via free radical generation, and the biological effects of these lasers from unusually
Braz J Med Biol Res. Publicado em: 28/07/2015
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2. The Compact Irradiator Modulus Designed for DNA Repair and Mutagenesis Studies in ISS Microgravity Environment Using UVA Emitted by Light-Emitting Diodes
ABSTRACT: This work presents the design and characteristics of a new compact ultraviolet (UV) irradiator used in a biological onboard space flight experiment. The experiment, called DRM, took place in the International Space Station research facility (ISS-13 expedition), during the Centenary Mission (Russian-Brazil) in March-April 2006. The DRM main objectiv
J. Aerosp. Technol. Manag.. Publicado em: 2014-09
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3. Avaliação da ação genotóxica induzida pela radiação ultravioleta solar na molécula de DNA / Evaluation of solar-ultraviolet radiation effects upon DNA molecule.
Nesse projeto, foi desenvolvido um sistema biológico que denominamos Dosímetro de DNA, com o objetivo de avaliar a ação genotóxica da radiação UV solar a partir da produção de lesões na molécula de DNA. Para determinar os diferentes tipos de danos, utilizamos enzimas de reparo de DNA e anticorpos específicos. Complementando estas análises, foram
Publicado em: 2009
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4. In situ hybridization with fluoresceinated DNA.
We have used fluorescein-11-dUTP in a nick-translation format to produce fluoresceinated human nucleic acid probes. After in situ hybridization of fluoresceinated DNAs to human metaphase chromosomes, the detection sensitivity was found to be 50-100 kb. The feasibility and the increase in detection sensitivity of microscopic imaging of in situ hybridized, flu
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5. Mutagenesis in Oocytes of DROSOPHILA MELANOGASTER. I. Scheduled Synthesis of Nuclear and Mitochondrial DNA and Unscheduled DNA Synthesis
As a model system for studying mutagenesis, the oocyte of Drosophila melanogaster has exhibited considerable complexity. Very few experiments have been conducted on the effect of exposing oocytes to chemical mutagens, presumably due to their lower mutational response relative to sperm and spermatids. This lower response may be due either to a change in prob
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6. Phage display of ScFv peptides recognizing the thymidine(6–4)thymidine photoproduct
Solar ultraviolet (UV) radiation induces DNA photoproducts in skin cells and is the predominant cause of human skin cancers. To understand human susceptibility to skin cancer and to facilitate the development of prevention measures, highly specific reagents to detect and quantitate UV-induced DNA adducts in human skin will be needed. One approach towards thi
Oxford University Press.
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7. Accelerator mass spectrometry in biomedical dosimetry: relationship between low-level exposure and covalent binding of heterocyclic amine carcinogens to DNA.
Accelerator mass spectrometry (AMS) is used to determine the amount of carcinogen covalently bound to mouse liver DNA (DNA adduct) following very low-level exposure to a 14C-labeled carcinogen. AMS is a highly sensitive method for counting long-lived but rare cosmogenic isotopes. While AMS is a tool of importance in the earth sciences, it has not been applie
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8. Long-Term Dosimetry of Solar UV Radiation in Antarctica with Spores of Bacillus subtilis
The main objective was to assess the influence of the seasonal stratospheric ozone depletion on the UV climate in Antarctica by using a biological test system. This method is based on the UV sensitivity of a DNA repair-deficient strain of Bacillus subtilis (TKJ 6321). In our field experiment, dried layers of B. subtilis spores on quartz discs were exposed in
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9. Purified scrapie prions resist inactivation by UV irradiation.
The development of effective purification protocols has permitted evaluation of the resistance of isolated scrapie prions to inactivation by UV irradiation at 254 nm. Prions were irradiated on ice with doses of UV light ranging up to 120,000 J/m2. UV dosimetry experiments, performed with Saccharomyces cerevisiae plasmid DNA or eucaryotic cells, indicated tha
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10. The oncogenic transforming potential of the passage of single α particles through mammalian cell nuclei
Domestic, low-level exposure to radon gas is considered a major environmental lung-cancer hazard involving DNA damage to bronchial cells by α particles from radon progeny. At domestic exposure levels, the relevant bronchial cells are very rarely traversed by more than one α particle, whereas at higher radon levels—at which epidemiological studies in uran
The National Academy of Sciences.