Double Labeled Water
Mostrando 1-6 de 6 artigos, teses e dissertações.
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1. Nutrição e ecologia nutricional de cervídeos brasileiros em cativeiro e no Parque Nacional das Emas - Goiás / Nutrition and nutritional ecology of brazilian cervids in captivity and in the Emas National Park - Goias
There are limited data on energy requirements of brazilian cervids. Thus, it is difficult to succeed in their management and reproduction in captivity. Knowing the energy requirements is also important to determine the necessary nutritional resources for their conservation in parks and reserves. The first objective was to study the nutritional requirements o
Publicado em: 2005
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2. Efficient synthesis of double dye-labeled oligodeoxyribonucleotide probes and their application in a real time PCR assay.
A fast cleaving non-nucleosidic tetramethylrhodamine dye-labeled support has been developed for automated synthesis of double dye-labeled oligodeoxyribonucleotides in high yield. A mixture (1:1:2) of t-butylamine:methanol:water is used for cleavage and deprotection of dye-labeled oligodeoxyribonucleotides without any degradation or modification of dyes and n
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3. Improved Double-Vial Radiorespirometric Technique for Mineralization of 14C-Labeled Substrates †
New procedures which simplify sample preparation and improve counting efficiency were developed for double-vial radiorespirometry. Under certain conditions, efficiency of fluor-NaOH-impregnated wicks was not stable, but was adversely affected by water vapor, NaOH concentration, and CO2 loading. Glass fiber wick material treated with a methanolic fluor-NaOH s
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4. Isotopic (18O) shift in 31P nuclear magnetic resonance applied to a study of enzyme-catalyzed phosphate—phosphate exchange and phosphate (oxygen)—water exchange reactions
An isotopic shift of the 31P nuclear magnetic resonance due to 18O bonded to phosphorus of 0.0206 ppm has been observed in inorganic orthophosphate and adenine nucleotides. Thus, the separation between the resonances of 31P18O4 and 31P16O4 at 145.7 MHz is 12 Hz and, in a randomized sample containing ∼50% 18O, all five 16O-18O species are resolved and separ
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5. Hydrogen peroxide-mediated alteration of the heme prosthetic group of metmyoglobin to an iron chlorin product: Evidence for a novel oxidative pathway
Treatment of metmyoglobin with H2O2 is known to lead to the crosslinking of an active site tyrosine residue to the heme [Catalano, C. E., Y. S. Choe, and P. R. Ortiz de Montellano (1989) J. Biol. Chem. 264, 10534–10541]. We have found in this study that this reaction also leads to an altered heme product not covalently bound to the protein. This prod
The National Academy of Sciences of the USA.
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6. Reversible, Temperature-Dependent Supramolecular Assembly of Aquaporin-4 Orthogonal Arrays in Live Cell Membranes
The shorter “M23” isoform of the glial cell water channel aquaporin-4 (AQP4) assembles into orthogonal arrays of particles (OAPs) in cell plasma membranes, whereas the full-length “M1” isoform does not. N-terminal residues are responsible for OAP formation by AQP4-M23 and for blocking of OAP formation in AQP4-M1. In investigating differences in OAP f
The Biophysical Society.