Double Mutant Cycles
Mostrando 1-12 de 14 artigos, teses e dissertações.
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1. Avaliação do padrão de degeneração e regeneração muscular em diferentes modelos murinos para distrofias musculares progressivas / Study of degeneration and regeneration pathways, in mice models for muscular dystrophies
The muscular dystrophies are a heterogeneous group of genetic diseases characterized by progressive and irreversible degeneration of skeletal muscles. Muscle weakness is the consequence of an imbalance between successive cycles of degeneration and regeneration, with further replacement of the degraded muscle fibers by adipose and connective tissues. Several
Publicado em: 2009
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2. Thermodynamic and structural studies of the head-to-tail complex of the muscular alpha-Tropomyosin / Estudos termodinâmicos e estruturais da interação cabeça-cauda da , alpha-tropomiosina muscular
Tropomiosina (Tm) é uma das proteínas que compõe o filamento fino (actina, Tm, Troponina) do sistema muscular esquelético e desempenha um importante papel na regulação da contração muscular. Tm é um coiled-coil de 284 resíduos que forma longos homopolímeros lineares através da sobreposição de onze resíduos entre os terminais de Tms adjacentes
Publicado em: 2008
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3. Structure of the interferon-receptor complex determined by distance constraints from double-mutant cycles and flexible docking
The pleiotropic activity of type I interferons has been attributed to the specific interaction of IFN with the cell-surface receptor components ifnar1 and ifnar2. To date, the structure of IFN has been solved, but not that of the receptor or the complex. In this study, the structure of the IFN-α2–ifnar2 complex was generated with a docking procedure, usin
The National Academy of Sciences.
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4. Substituent effects on cation–π interactions: A quantitative study
A synthetic supramolecular complex has been adapted to quantify cation–π interactions in chloroform by using chemical double-mutant cycles. The interaction of a pyridinium cation with the π-face of an aromatic ring is found to be very sensitive to the π-electron density. Electron-donating substituents lead to a strong attractive interaction (−8 kJ/mol
National Academy of Sciences.
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5. Glp-3 Is Required for Mitosis and Meiosis in the Caenorhabditis Elegans Germ Line
The germ line is the only tissue in Caenorhabditis elegans in which a stem cell population continues to divide mitotically throughout life; hence the cell cycles of the germ line and the soma are regulated differently. Here we report the genetic and phenotypic characterization of the glp-3 gene. In animals homozygous for each of five recessive loss-of-functi
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6. Determination of the folding transition states of barnase by using ΦI-value-restrained simulations validated by double mutant ΦIJ-values
The protein barnase folds from the denatured state into its native conformation via a high-energy intermediate. Using ΦI-values determined experimentally from single-point mutations as restraints in all-atom molecular dynamics simulations, we have determined ensembles of structures corresponding to the transition states for the formation of the folding inte
National Academy of Sciences.
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7. Cyclins E1 and E2 are required for endoreplication in placental trophoblast giant cells
In mammalian cells, cyclin E–CDK2 complexes are activated in the late G1 phase of the cell cycle and are believed to have an essential role in promoting S-phase entry. We have targeted the murine genes CCNE1 and CCNE2, encoding cyclins E1 and E2. Whereas single knockout mice were viable, double knockout embryos died around midgestation. Strikingly, however
Oxford University Press.
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8. Vps10p Cycles between the TGN and the Late Endosome via the Plasma Membrane in Clathrin Mutants
Clathrin-coated vesicles mediate the transport of the soluble vacuolar protein CPY from the TGN to the endosomal/prevacuolar compartment. Surprisingly, CPY sorting is not affected in clathrin deletion mutant cells. Here, we have investigated the clathrin-independent pathway that allows CPY transport to the vacuole. We find that CPY transport is mediated by t
The American Society for Cell Biology.
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9. Mechanism of rescue of common p53 cancer mutations by second-site suppressor mutations
The core domain of p53 is extremely susceptible to mutations that lead to loss of function. We analysed the stability and DNA-binding activity of such mutants to understand the mechanism of second-site suppressor mutations. Double-mutant cycles show that N239Y and N268D act as ‘global stability’ suppressors by increasing the stability of the cancer mutan
Oxford University Press.
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10. Construction of mutant and chimeric genes using the polymerase chain reaction.
In the polymerase chain reaction (PCR) the specific amplification of a small segment of DNA within a complex DNA sample is effected by repeated cycles of DNA denaturation and enzymatic synthesis primed by two oligonucleotides complementary to regions within opposite strands of the DNA. In this report a simple and efficient method is described in which PCR me
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11. Infection of Ducklings with Virus Particles Containing Linear Double-Stranded Duck Hepatitis B Virus DNA: Illegitimate Replication and Reversion
Double-stranded linear DNA is synthesized as a minor viral DNA species by all hepadnaviruses. In a previous study (W. Yang and J. Summers, J. Virol. 69:4029–4036, 1995) we showed that virus particles containing linear DNA of the duck hepatitis B virus (DHBV) could initiate an infection of primary duck hepatocytes. In cells infected by linear DNA containing
American Society for Microbiology.
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12. Regulation of the Anaphase-promoting Complex/Cyclosome by bimAAPC3 and Proteolysis of NIMA
Surprisingly, although highly temperature-sensitive, the bimA1APC3 anaphase-promoting complex/cyclosome (APC/C) mutation does not cause arrest of mitotic exit. Instead, rapid inactivation of bimA1APC3 is shown to promote repeating oscillations of chromosome condensation and decondensation, activation and inactivation of NIMA and p34cdc2 kinases, and a
The American Society for Cell Biology.