Dsred
Mostrando 1-12 de 38 artigos, teses e dissertações.
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1. Agrotransformação de Phytophthora nicotianae: um método simplificado e otimizado
RESUMO Phytophthora nicotianae é um fitopatógeno responsável por danos em culturas e ecossistemas naturais em todo o mundo. P. nicotianae está relacionada às doencas: gomose e podridao de raízes e o manejo destas doencas depende principalmente de certificação de mudas e erradicação de plantas infectadas. No entanto, pouco se sabe sobre a estratégi
Summa phytopathol.. Publicado em: 2016-09
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2. Colonização de sementes de milho por duas espécies de Stenocarpella transformados com proteínas fluorescentes e avaliadas por microscopia eletrônica de varredura
Stenocarpella maydis e Stenocarpella macrospora, espécies causadoras de manchas foliares, podridões em plantas e grãos ardidos de milho, podem ser transportadas e dispersas para áreas produtoras através das sementes. O objetivo foi transformar isolados de espécies de Stenocarpella com GFP e DsRed e correlacionar diferentes potenciais de inóculo com o
J. Seed Sci.. Publicado em: 2014-06
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3. DsRed2 transient expression in Culex quinquefasciatus mosquitoes
Culex quinquefasciatus mosquitoes have been successfully genetically modified only once, despite the efforts of several laboratories to transform and establish a stable strain. We have developed a transient gene expression method, in Culex, that delivers plasmid DNA directly to the mosquito haemolymph and additional tissues. We were able to express DsRed2 f
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-06
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4. Fusão Homotípica e Heterotípica entre Vacúolos Parasitóforos de Leishmania spp / Homotypic and Heterotypic Fusion between Leishmania spp. Parasitophorous Vacuoles
Most non-viral intracellular pathogens gain entrance into human and animal host cells by classic or modifIed phagocytosis and are thus lodged in phagosomes which they may or not continue to occupy in the course of infection. Their survival and multiplication within host cells depend on modulation of the compositional and functional phenotypes of the phagosom
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 22/02/2011
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5. Interaction between human cytomegalovirus UL136 protein and ATP1B1 protein
Interplay between the host and human cytomegalovirus (HCMV) has a pivotal role in the outcome of infection. A region (referred to as UL/b’) present in the Toledo strain of HCMV and low passage clinical isolates contains 19 additional genes, which are absent in the highly passaged laboratory strain AD169. Products of the UL/b’ genes may determine the mani
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-12
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6. Evidence for the endophytic colonization of Phaseolus vulgaris(common bean) roots by the diazotroph Herbaspirillum seropedicae
Herbaspirillum seropedicae is an endophytic diazotrophic bacterium, which associates with important agricultural plants. In the present study, we have investigated the attachment to and internal colonization of Phaseolus vulgaris roots by the H. seropedicae wild-type strain SMR1 and by a strain of H. seropedicae expressing a red fluorescent protein (DsRed) t
Braz J Med Biol Res. Publicado em: 2011-03
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7. Transformação por eletroporação de Herbaspirillum seropedicae HRC54, presente no inoculante recomendado para cana-de-açúcar, com gene repórter DsRED.
2010
SEMANA CIENTÍFICA JOHANNA DÖBEREINER. Publicado em: 2011
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8. Transformation of Fusarium oxysporum f sp. vasinfectum with GFP and DsRed gene markers. / TRANSFORMAÃÃO DE Fusarium oxysporum f. sp. vasinfectum COM OS GENES MARCADORES GFP e DsRed.
Fusarium oxysporum f sp. vasinfectum, the casual agent of fusarium wilt disease of cotton, is frequent in most cottonâproducing regions in Brazil. The fungus infects roots, colonizes the rhizomes and eventually blocks the vascular system of the pseudostems, leading to the plant collapse. The Green and Red Fluorescent Proteins (GFP and DsRed) emit green and
Publicado em: 2009
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9. TransformaÃÃo de Stenocarpella maydis com os genes marcadores GFP e DsRed e patogenicidade dos transformados em sementes de milho / Transformation of Stenocarpella maydis with genes markers GFP and DsRed and pathogenicity of transformed into maize seeds
One of the fungi which can associate to maize seeds is Stenocarpella maydis, causal agent of stalk and ear rot, which is responsible by considerable losses in producing regions of this crop in Brazil. From literature, there is little information on the mechanisms of infection and on the transmission dynamics of this fungus in relation to maize seeds. For thi
Publicado em: 2009
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10. Criação de uma série de lentiventores para transferência gênica estável e regulada
A transferência gênica baseada em retrovírus permite o carregamento e integração de um material genético exógeno ao genoma de uma célula alvo, o que permite a expressão estável do transgene tanto in vitro quanto in vivo. Até o momento não existiam lentivetores que permitam a clonagem de diferentes genes sob promotores de expressão gênica regula
Publicado em: 2009
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11. Early colonization pattern of maize (Zea mays L. Poales, Poaceae) roots by Herbaspirillum seropedicae (Burkholderiales, Oxalobacteraceae)
The bacterium Herbaspirillum seropedicae is an endophytic diazotroph found in several plants, including economically important poaceous species. However, the mechanisms involved in the interaction between H. seropedicae and these plants are not completely characterized. We investigated the attachment of Herbaspirillum to maize roots and the invasion of the r
Genetics and Molecular Biology. Publicado em: 03/09/2008
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12. Characterization of endophytic, nitrogen-fixing bacteria isolated from Saccharum sp. (sugarcane) cultivated under organic fertilization or nitrogenated fertlization and no fertilization. / Caracterização de bactécias fixadoras de nitrogênio endofíticas isoladas de Saccharum sp. (cana-de-açúcar) cultivadas sob adubação orgânica ou fertilizante nitrogenado ou sem adubação.
In the present study, endophytic bacterial diversity has been searched using both microbiologic and molecular methods. Microorganisms were isolated from sugarcane root, shoot and leaf. 150 isolates were identified by 16S rDNA sequencing. 18 genera were found and only 4 were present in sugarcane submitted to the three treatments. The greatest genera diversity
Publicado em: 2008