Ef Hand Domain
Mostrando 1-12 de 45 artigos, teses e dissertações.
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1. Estudos estruturais e termodinâmicos de centrinas BeCen1 e BeCen3 do fungo Blastocladiella emersonii / Structural and Thermodynamics Studies of Blastocladiella Emersonii Centrins
Centrinas são proteínas componentes essenciais nos centro organizadores de microtubulos em diversos organismos. Pertencem à família das proteínas EF-Hand ligantes de cálcio e podem ser divididas em duas subfamílias: uma definida pela centrina da alga Chlamydomonas reinhardtii CrCenp, associada a funções contráteis, e a outra pela centrina da levedu
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/05/2011
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2. Aspectos genéticos no transtorno do pânico
A herdabilidade estimada do Transtorno do Pânico (TP) é de 28 a 43%, embora ainda permaneça como questão de pesquisa quais os genes envolvidos na sua etiologia. A busca dos genes candidatos para os estudos de associação geralmente é baseada em moléculas envolvidas no tratamento farmacológico, nos agentes que induzem ataques de pânico ou nos estudos
Publicado em: 2008
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3. Clonagem, expressão e caracterização imunológica da proteína Pb40r presente na fração protetora F0 de paracoccidioides brasiliensis
Paracoccidioidomicose (PCM) é uma das mais importantes micoses endêmicas da América Latina; ela é usualmente diagnosticada pela observação e/ou isolamento do seu agente etiológico Paracoccidioides brasiliensis, assim como por uma grande variedade de métodos imunológicos. Embora esses últimos sejam efetivos, dois grandes problemas, reação cruzada
Publicado em: 2007
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4. Expressão e purificação de proteinas relacionadas a epilepsia / Expression and purification of epilepsy related proteins
Non-ion channel genes, as LGI1 and EFHC1, have been shown to indirectly affect ion channel currents in genetically determined epilepsy syndromes. LGI1 (Leucine-rich, glioma inactivated gene 1) is linked to a rare form of partial epilepsy (autosomal dominant partial epilepsy with auditory features, ADPEAF). Recently, LGI1 protein was associated with ADAM22 in
Publicado em: 2007
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5. Role of essential light chain EF hand domains in calcium binding and regulation of scallop myosin.
The specific Ca2+ binding site that triggers contraction of molluscan muscle requires the presence of an essential light chain (ELC) from a Ca2+ binding myosin. Of the four EF hand-like domains in molluscan ELCs, only domain III has an amino acid sequence predicted to be capable of binding Ca2+. In this report, we have used mutant ELCs to locate the Ca2+ bin
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6. Crystal structure of elongation factor P from Thermus thermophilus HB8
Translation elongation factor P (EF-P) stimulates ribosomal peptidyltransferase activity. EF-P is conserved in bacteria and is essential for cell viability. Eukarya and Archaea have an EF-P homologue, eukaryotic initiation factor 5A (eIF-5A). In the present study, we determined the crystal structure of EF-P from Thermus thermophilus HB8 at a 1.65-Å resoluti
National Academy of Sciences.
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7. The cross-reactive calcium-binding pollen allergen, Phl p 7, reveals a novel dimer assembly
The timothy grass pollen allergen Phl p 7 assembles most of the IgE epitopes of a novel family of 2 EF-hand calcium-binding proteins and therefore represents a diagnostic marker allergen and vaccine candidate for immunotherapy. Here we report the first three-dimensional structure of a representative of the 2 EF-hand allergen family, Phl p 7, in the calcium-b
Oxford University Press.
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8. Phosphorylation and calcium binding properties of an Arabidopsis GF14 brain protein homolog.
Arabidopsis GF14 omega was originally described because of its apparent association with a DNA-protein complex; it is a member of the 14-3-3 kinase regulatory protein family that is conserved throughout eukaryotes. Here, we demonstrated that recombinant GF14 omega is expressed in Escherichia coli as a dimer. Blot binding and electrophoretic mobility shift an
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9. Analysis of the cytoplasmic interaction between polycystin-1 and polycystin-2
Autosomal dominant polycystic kidney disease (ADPKD) arises following mutations of either Pkd1 or Pkd2. The proteins these genes encode, polycystin-1 (PC1) and polycystin-2 (PC2), form a signaling complex using direct intermolecular interactions. Two distinct domains in the C-terminal tail of PC2 have recently been identified, an EF-hand and a coiled-coil do
American Physiological Society.
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10. RGS3 mediates a calcium-dependent termination of G protein signaling in sensory neurons
G proteins modulate synaptic transmission. Regulators of G protein signaling (RGS) proteins accelerate the intrinsic GTPase activity of Gα subunits, and thus terminate G protein activation. Whether RGS proteins themselves are under cellular control is not well defined, particularly in native cells. In dorsal root ganglion neurons overexpressing RGS3, we
National Academy of Sciences.
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11. The END3 gene encodes a protein that is required for the internalization step of endocytosis and for actin cytoskeleton organization in yeast.
Two Saccharomyces cerevisiae mutants, end3 and end4, defective in the internalization step of endocytosis, have previously been isolated. The END3 gene was cloned by complementation of the temperature-sensitive growth defect caused by the end3 mutation and the END3 nucleotide sequence was determined. The END3 gene product is a 40-kDa protein that has a putat
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12. Alpha-sarcin cleavage of ribosomal RNA is inhibited by the binding of elongation factor G or thiostrepton to the ribosome.
The translocation reaction catalyzed by elongation factor G (EF-G) is inhibited either by alpha-sarcin cleavage of 23S rRNA or by the binding of thiostrepton to the E. coli ribosome. Here we show that the transitory binding of EF-G and GDP to the ribosome inhibited the rate of alpha-sarcin cleavage and that stabilization of this binding with fusidic acid com