Electroporation
Mostrando 1-12 de 450 artigos, teses e dissertações.
-
1. Evaluation of the Current in the Cell Membrane for Numerical Simulations of Electroporation
Abstract This study presents a rigorous numerical analysis of the current in the cell membrane, subjected to a uniform electric field, and its impact in the pore formation. The numerical model considers a single cell composed of uniform membrane and cytoplasm, in a suspension medium. The current in the cell membrane is calculated using two different approach
Journal of Microwaves, Optoelectronics and Electromagnetic Applications. Publicado em: 2022
-
2. Production of recombinant human epidermal growth factor in Pichia pastoris
Abstract This study was carried out to express human epidermal growth factor (hEGF) in Pichia pastoris GS115. For this aim, the hEGF gene was cloned into the pPIC9K expression vector, and then integrated into P. pastoris by electroporation. ELISA-based assay showed that the amount of hEGF secreted into the medium can be affected by the fermentation condition
Braz. J. Microbiol.. Publicado em: 2017-06
-
3. First characterization of a Providencia stuartii clinical isolate from a Tunisian intensive care unit coproducing VEB-1-a, OXA-2, qnrA6 and aac(6')-Ib-cr determinants
A clinical Providencia stuartii isolate SM662 was recovered from a patient hospitalized in the intensive care unit at the Military hospital, Tunisia. This isolate was resistant to penicillins, cephalosporins, aminoglycosides and fluoroquinolones. A marked in vitro synergy between ceftazidime or cefotaxime and amoxicillin-clavulanic acid on Mueller-Hinton aga
Braz J Infect Dis. Publicado em: 2014-04
-
4. A detailed description of an economical setup for electroporation of chick embryos in ovo
One of the challenges of the postgenomic era is characterizing the function and regulation of specific genes. For various reasons, the early chick embryo can easily be adopted as an in vivo assay of gene function and regulation. The embryos are robust, accessible, easily manipulated, and maintained in the laboratory. Genomic resources centered on vertebrate
Braz J Med Biol Res. Publicado em: 18/09/2013
-
5. Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot
Blister spot (Colletotrichum gloeosporioides) is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches) leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring
Braz. J. Microbiol.. Publicado em: 2012-09
-
6. Hibridização in situ em espermatozóides bovinos tratados com DNA exógeno: estudo experimental / In situ hybridization in bovine sperm treated with exogenous DNA: an experimental study.
Muitas das técnicas utilizadas para gerar animais transgênicos são caras e laboriosas. Neste contexto, a transferência gênica mediada por espermatozóides (TGME) pode ser uma alternativa para a produção em larga escala de animais transgênicos. Estudos de SMGT têm seu foco no número de cópias de DNA incorporada pelo espermatozóide. Por isso, há p
Publicado em: 2010
-
7. Análise do transcritoma de etiquetas de sequências expressas da hipófise e parte do cérebro do tambaqui (Colossoma macropomum) e expressão do cDNA do hormônio de crescimento em Pichia pastoris
The tambaqui, Colossoma macropomum, is a fish of great commercial value to the Amazon region and represents an emblematic species taking into account the studies carried out on its biology, physiology and genetics. Due to its importance, the aim of this study was to construct and characterize a cDNA library of mixed parts of the hypophysis and the brain in o
Publicado em: 2009
-
8. Genic transfer in sperm cells in Mus musculus and Ramdia quelem / Transferência gênica em células espermáticas de Mus musculus e Ramdia quelen
Transgenic animals have been used as biological models in studies of the genes functions and their mechanisms of action, as well as to improve animal production. Researchers are trying to produce transgenic animals that will be organs donors in xenotransplants. Another use of the transgenic animal is in the production of recombinant proteins for pharmaceutic
Publicado em: 2009
-
9. Identificação e caracterização funcional dos elementos cis-regulatorios da miostatina / Identification and functional characterization of the cis-regulatory elements of myostatin
The Myostatin protein (also known as GDF8) is a member of the transforming growth factor-β (TGF-β) superfamily and is expressed almost exclusively in skeletal muscle, both in the embryo and in the adult, where the protein circulates in the blood flow. It was initially identified in 1997 by MCPHERRON et al., and since then many studies have been dem
Publicado em: 2009
-
10. Estudo comparativo de protocolos de imunização gênica: eletroporação aumenta consistentemente a resposta imune humoral / Comparative study of protocols of gene immunization: electroporation consistently improves the humoral immune response
A imunização gênica pode contornar alguns problemas encontrados na imunização protéica, quais sejam as dificuldades na obtenção do antígeno purificado e a reatividade da resposta imune (r.i.) com a forma nativa do antígeno. O gene que codifica o antígeno de interesse, carregado por um vetor, é injetado no animal e a proteína é expressa in vivo,
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/03/2008
-
11. Transferência gênica in vivo por meio de eletroporação em músculo sóleo de ratos wistar. / In vivo gene transfer by electroporation in soleus muscle from wistar rats.
The use of electrical pulses for gene transfer has been successfully used to reach significant levels of gene expression in vitro and in vivo. Therefore electroporation can be considered an important device to get further insight on biological mechanisms and also to treatment. The aim of the present work is to achieve a high level of gene transfer throughout
Publicado em: 2008
-
12. Construção de vetor oriC de Mycoplasma hyopneumoniae uma ferramenta para estudos genéticos do agente da pneumonia enzoótica suína.
Mycoplasma hyopneumoniae é o agente etiológico da pneumonia enzoótica, enfermidade distribuída mundialmente em rebanhos suínos nas fases de crescimento e terminação. A pneumonia enzoótica tem sido considerada uma das mais importantes causas de perdas econômicas na suinocultura mundial. Tendo em vista a disponibilidade dos genomas completos de três
Publicado em: 2008