Endochitinase
Mostrando 1-12 de 45 artigos, teses e dissertações.
-
1. The chitinase C gene PsChiC from Pseudomonas sp. and its synergistic effects on larvicidal activity
Pseudomonas sp. strain TXG6-1, a chitinolytic gram-negative bacterium, was isolated from a vegetable field in Taixing city, Jiangsu Province, China. In this study, a Pseudomonas chitinase C gene (PsChiC) was isolated from the chromosomal DNA of this bacterium using a pair of specific primers. The PsChiC gene consisted of an open reading frame of 1443 nucleot
Genet. Mol. Biol.. Publicado em: 21/08/2015
-
2. Chitinase activities, scab resistance, mycorrhization rates and biomass of own-rooted and grafted transgenic apple
This study investigated the impact of constitutively expressed Trichoderma atroviride genes encoding exochitinase nag70 or endochitinase ech42 in transgenic lines of the apple cultivar Pinova on the symbiosis with arbuscular mycorrhizal fungi (AMF). We compared the exo- and endochitinase activities of leaves and roots from non-transgenic Pinova and the trans
Genetics and Molecular Biology. Publicado em: 2012
-
3. Purificação parcial das quitinases, Pbcts1 e Pbcts2, do fungo Paracoccidiodes brasiliensis / Partial purification of chitinases, and Pbcts1 Pbcts2, fungus Paracoccidioides brasiliensis
Paracoccidoides brasiliensis is a human pathogenic dimorphic fungus. The recombinant chitinase from P. brasiliensis, Pbcts1r, was overexpressed in Escherichia coli using pET-32a (+) as vector. The enzyme was produced as inclusion bodies and became soluble by Sarkosyl being purified by a single step using a Ni-NTA resin. Pbcts1r showed activity against 4-MU-(
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 03/04/2008
-
4. Estudos de sinalização celular em Hypocrea jecorina (Trichoderma reesei) durante a expressão dos genes de celulase (cbh1 e cbh2) em presença de celulose e soforose e durante o antagonismo contra Pythium ultimum
Trichoderma reesei (Hypocrea jecorina) is widely used in industry and its potential for use in agriculture as a biocontrol agent against phytopathogenic fungi has just begun to be explored. The adenylate cyclase activating subgroup III Ga proteinencoding gene gna3 of T. reesei was cloned to study its possible role in the control of cellulose and sophorose to
Publicado em: 2008
-
5. AvaliaÃÃo bioquimica da diversidade genÃtica de linhagens de Chromobacterium violaceum e caracterizaÃÃo molecular do gene de quitinase
Chromobacterium violaceum à uma bactÃria Gram-negativa, pigmentada, aerÃbica facultativa, que vive em regiÃes de climas tropicais e subtropicais, encontrada principalmente, em solos e rios. Seu potencial biotecnolÃgico demonstrado foi atravÃs do genoma seqÃenciado da C. violaceum ATCC 12472, que apresentou genes desconhecidos relacionados à biossÃnt
Publicado em: 2007
-
6. Improved production of Trichoderma harzianum endochitinase by expression in Trichoderma reesei.
The chromosomal endochitinase gene (ThEn-42) of the mycoparasite fungus Trichoderma harzianum P1 was isolated and overexpressed in the filamentous fungus Trichoderma reesei under the promoter of the major cellulase gene cbhl1. The host strain RutC-30 did not produce any endogenous endochitinase activity. The prepro region of the T harzianum endochitinase was
-
7. Synergistic activity of a Bacillus thuringiensis delta-endotoxin and a bacterial endochitinase against Spodoptera littoralis larvae.
In an attempt to increase the insecticidal effect of the delta-endotoxin crystal protein CryIC on the relatively Cry-insensitive larvae of Spodoptera littoralis, a combination of CryIC and endochitinase was used. CryIC comprising the first 756 amino acids from Bacillus thuringiensis K26-21 and endochitinase ChiAII encoded by Serratia marcescens were separate
-
8. Role of the Trichoderma harzianum Endochitinase Gene, ech42, in Mycoparasitism
The role of the Trichoderma harzianum endochitinase (Ech42) in mycoparasitism was studied by genetically manipulating the gene that encodes Ech42, ech42. We constructed several transgenic T. harzianum strains carrying multiple copies of ech42 and the corresponding gene disruptants. The level of extracellular endochitinase activity when T. harzianum was grown
American Society for Microbiology.
-
9. A carrot somatic embryo mutant is rescued by chitinase.
At the nonpermissive temperature, somatic embryogenesis of the temperature-sensitive (ts) carrot cell mutant ts11 does not proceed beyond the globular stage. This developmental arrest can be lifted by the addition of proteins secreted by wild-type cells to the culture medium. From this mixture of secreted proteins, a 32-kD glycoprotein, designated extracellu
-
10. Rhizobium Lipooligosaccharides Rescue a Carrot Somatic Embryo Mutant.
At a nonpermissive temperature, somatic embryos of the temperature-sensitive (ts) carrot cell mutant ts11 only proceed beyond the globular embryo stage in the presence of medium conditioned by wild-type embryos. The causative component in the conditioned medium has previously been identified as a 32-kD acidic endochitinase. In search of a function for this e
-
11. Nucleotide sequence of a Brassica napus endochitinase gene.
-
12. Primary structure of an endochitinase mRNA from Solanum tuberosum.