Epoxide Hydrolase
Mostrando 1-12 de 56 artigos, teses e dissertações.
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1. Simultaneous Multienzymatic Screening with Fluorogenic Probes
The simultaneous screening of multiple enzyme activities in a single assay has numerous advantages over the traditional format, since it decreases sampling errors, allows savings in reagents and consumables and reduces the time and labor required to conduct the assays. In the present study, a direct and sensitive assay for the simultaneous detection of epoxi
J. Braz. Chem. Soc.. Publicado em: 2018-05
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2. Enzymatic potential of heterotrophic bacteria from a neutral copper mine drainage
Abstract Copper mine drainages are restricted environments that have been overlooked as sources of new biocatalysts for bioremediation and organic syntheses. Therefore, this study aimed to determine the enzymatic activities (esterase, epoxide hydrolase and monooxygenase) of 56 heterotrophic bacteria isolated from a neutral copper mine drainage (Sossego Mine,
Braz. J. Microbiol.. Publicado em: 2016-12
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3. Triagem de enzimas associadas à biotransformação de hidrocarbonetos a partir de metagenoma de sedimentos contaminados com petroléo e metais pesados / Screening of Enzymes Related to Biotransformation of Hydrocarbons from Metagenome of Contaminated Sediments with Oil and Heavy Metals
A metagenômica trouxe novas perspectivas ao estudo de comunidades microbianas no ambiente, permitindo explorar tanto a diversidade taxonômica de microrganismos ainda não-cultivados, como o acesso direto a genes e vias metabólicas. Neste trabalho, foram construídas bibliotecas metagenômicas a partir de amostras de sedimentos de mangue da Baía de Guanab
Publicado em: 2009
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4. Limonene-1,2-Epoxide Hydrolase from Rhodococcus erythropolis DCL14 Belongs to a Novel Class of Epoxide Hydrolases
An epoxide hydrolase from Rhodococcus erythropolis DCL14 catalyzes the hydrolysis of limonene-1,2-epoxide to limonene-1,2-diol. The enzyme is induced when R. erythropolis is grown on monoterpenes, reflecting its role in the limonene degradation pathway of this microorganism. Limonene-1,2-epoxide hydrolase was purified to homogeneity. It is a monomeric cytopl
American Society for Microbiology.
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5. Effect of phenobarbital on the level of translatable rat liver epoxide hydrolase mRNA.
Liver poly(A)+RNA isolated from untreated and phenobarbital-treated rats has been translated in the rabbit reticulocyte cell-fre system in order to determine the level of translationally active epoxide hydrolase (EC 3.3.2.3) mRNA. The in vitro translation systems were immunoprecipitated with rabbit IgG prepared against purified epoxide hydrolase, and the amo
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6. Detoxification of environmental mutagens and carcinogens: Structure, mechanism, and evolution of liver epoxide hydrolase
The crystal structure of recombinant murine liver cytosolic epoxide hydrolase (EC 3.3.2.3) has been determined at 2.8-Å resolution. The binding of a nanomolar affinity inhibitor confirms the active site location in the C-terminal domain; this domain is similar to that of haloalkane dehalogenase and shares the α/β hydrolase fold. A structure-based mechani
The National Academy of Sciences.
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7. Potent urea and carbamate inhibitors of soluble epoxide hydrolases
The soluble epoxide hydrolase (sEH) plays a significant role in the biosynthesis of inflammation mediators as well as xenobiotic transformations. Herein, we report the discovery of substituted ureas and carbamates as potent inhibitors of sEH. Some of these selective, competitive tight-binding inhibitors with nanomolar Ki values interacted stoichiometrically
The National Academy of Sciences.
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8. Structure of Rhodococcus erythropolis limonene-1,2-epoxide hydrolase reveals a novel active site
Epoxide hydrolases are essential for the processing of epoxide-containing compounds in detoxification or metabolism. The classic epoxide hydrolases have an α/β hydrolase fold and act via a two-step reaction mechanism including an enzyme–substrate intermediate. We report here the structure of the limonene-1,2-epoxide hydrolase from Rhodococcus erythropoli
Oxford University Press.
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9. Leukotriene A4 hydrolase: mapping of a henicosapeptide involved in mechanism-based inactivation.
Leukotriene A4 (LTA4) hydrolase [7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9 ,11,14-tetraenoate hydrolase; EC 3.3.2.6] is a bifunctional zinc metalloenzyme which converts LTA4 into the chemotactic agent leukotriene B4 (LTB4). Suicide inactivation, a typical feature of LTA4 hydrolase/aminopeptidase, occurs via an irreversible, apparently mechanism-based, covale
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10. Isolation and Characterization of the Epoxide Hydrolase-Encoding Gene from Xanthophyllomyces dendrorhous
The epoxide hydrolase (EH)-encoding gene (EPH1) from the basidiomycetous yeast Xanthophyllomyces dendrorhous was isolated. The genomic sequence has a 1,236-bp open reading frame which is interrupted by eight introns that encode a 411-amino-acid polypeptide with a calculated molecular mass of 46.2 kDa. The amino acid sequence is similar to that of microsomal
American Society for Microbiology.
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11. Microsomal epoxide hydrolase, glutathione S‐transferase P1, traffic and childhood asthma
BMJ Group.
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12. Nucleotide and deduced amino acid sequence of human liver microsomal epoxide hydrolase.