Fluorescent In Situ Hybridization
Mostrando 1-12 de 314 artigos, teses e dissertações.
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1. The structuring role of macrophytes on plankton community composition and bacterial metabolism in a large subtropical shallow lake
Resumo Objetivo Nós objetivamos investigar mudanças nos principais grupos do plâncton (bacterioplâncton, fitoplâncton e zooplâncton) e no metabolismo do bacterioplâncton ao longo de um transecto cobrindo uma zona litorânea dominada por macrófitas para uma zona pelágica dominada por fitoplâncton na lagoa Mangueira, uma lagoa extensa, rasa e subtro
Acta Limnol. Bras.. Publicado em: 03/06/2019
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2. Characterization and chromosomal mapping of the DgmarMITE transposon in populations of Dichotomius (Luederwaldtinia) sericeus species complex (Coleoptera: Scarabaeidae)
Abstract Transposable elements are dispersed repetitive DNA sequences that can move within the genome and are related to genome and chromosome evolution, adaptation, and speciation. The aim of this study was to characterize and determine the chromosomal location and accumulation of a Mariner-like element in populations of four phylogenetically related specie
Genet. Mol. Biol.. Publicado em: 04/06/2018
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3. Karyotype and genome size comparative analyses among six species of the oilseed-bearing genus Jatropha (Euphorbiaceae)
Abstract Jatropha is an important genus of Euphorbiaceae, with species largely used for various purposes, including the manufacturing of soaps and pharmaceutical products and applications in the bioenergetic industry. Although there have been several studies focusing J. curcas in various aspects, the karyotype features of Jatropha species are poorly known. T
Genet. Mol. Biol.. Publicado em: 14/05/2018
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4. Comparative cytogenetic analysis in Erythrolamprus snakes (Serpentes: Dipsadidae) from Argentina
ABSTRACT We described the karyotypes of five snake taxa from Argentina: Erythrolamprus almadensis, E. ceii, E. poecilogyrus caesius, E. p. schotti and E. p. sublineatus, and also intergrading individuals between the last two subspecies by conventional staining, chromosome bandings and fluorescent in situ hybridization (FISH) with 28S ribosomal DNA probes. Er
An. Acad. Bras. Ciênc.. Publicado em: 2018-04
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5. Protocol for chromosome-specific probe construction using PRINS, micromanipulation and DOP-PCR techniques
ABSTRACT Chromosome-specific probes have been widely used in molecular cytogenetics, being obtained with different methods. In this study, a reproducible protocol for construction of chromosome-specific probes is proposed which associates in situ amplification (PRINS), micromanipulation and degenerate oligonucleotide-primed PCR (DOP-PCR). Human lymphocyte cu
An. Acad. Bras. Ciênc.. Publicado em: 07/12/2017
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6. Genetic aberrations in multiple myeloma characterized by cIg-FISH: a Brazilian context
Genetic abnormalities are critical prognostic factors for patients diagnosed with multiple myeloma (MM). This retrospective, multicenter study aimed to contribute with the genetic and clinical characterization of MM patients in a country with continental dimensions such as Brazil. Genetic abnormalities were assessed by cIg-fluorescent in situ hybridization (
Braz J Med Biol Res. Publicado em: 08/04/2016
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7. Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells
Lung cancer often exhibits molecular changes, such as the overexpression of the ErbB1 gene that encodes epidermal growth factor receptor (EGFR). ErbB1 amplification and mutation are associated with tumor aggressiveness and low response to therapy. The aim of the present study was to design a schedule to synchronize the cell cycle of A549 cell line (a non-sma
Braz J Med Biol Res. Publicado em: 06/03/2015
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8. Large scale distribution of bacterial communities in the upper Paraná River floodplain
A bacterial community has a central role in nutrient cycle in aquatic habitats. Therefore, it is important to analyze how this community is distributed throughout different locations. Thirty-six different sites in the upper Paraná River floodplain were surveyed to determine the influence of environmental variable in bacterial community composition. The site
Braz. J. Microbiol.. Publicado em: 2014-12
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9. Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed
Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the "river buffaloes" with 2n = 50 and the "swamp buffaloes", 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bear
Genet. Mol. Biol.. Publicado em: 2014-06
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10. Human molecular cytogenetics: from cells to nucleotides
The field of cytogenetics has focused on studying the number, structure, function and origin of chromosomal abnormalities and the evolution of chromosomes. The development of fluorescent molecules that either directly or via an intermediate molecule bind to DNA has led to the development of fluorescent in situ hybridization (FISH), a technology linking cytog
Genet. Mol. Biol.. Publicado em: 2014
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11. A remarkable autosomal heteromorphism in Pseudoryzomys simplex 2n = 56; FN = 54-55 (Rodentia, Sigmodontinae)
Pseudoryzomys simplex, the false rice rat, is a monotypic genus of the Oryzomyini tribe (Sigmodontinae) distributed in part of Bolivia, Paraguay, Argentina and Brazil. Its diploid number has been described as 56 acrocentric chromosomes decreasing in size and no karyotype figure has been depicted. Herein, we present karyotypic data on P. simplex, including ch
Genet. Mol. Biol.. Publicado em: 2013
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12. ASPECTOS EVOLUTIVOS E DIFERENCIAÇÃO DE POPULAÇÕES DE Astyanax scabripinnis (CHARACIDAE, INCERTAE SEDIS)
The species complex Astyanax scabripinnis are composed by fish with wide geographic distribution. Their populations are often isolated in small streams, separated by thousands of years, making it a very interesting system to study evolution. The use of different markers is crucial, since they allow inferences on the identification of individuals and therefor
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 08/02/2012