Genetic Recombination
Mostrando 1-12 de 2353 artigos, teses e dissertações.
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1. Genetic linkage map and mapping of the locus of biological nitrogen fixation inefficiency in cowpea
ABSTRACT. The objectives of the present study were to construct a cowpea genetic map using the F2 population resulting from the cross IC-1 x BRS Marataoã, based on single nucleotide polymorphism (SNP) markers, and to map the cpi gene, with additional reference to introgression with the consensus map of species, aiming to identify markers for assisted select
Acta Sci., Agron.. Publicado em: 23/09/2019
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2. Genetic variability and breeding potential of Flintisa Composite of maize in two levels of technology
Abstract The purpose this study was to verify the breeding potential of Flintisa Composite for low (LT) and high (HT) technology of cultivation, and the best selection strategy to be adopted. For this reason half-sib progenies were evaluated in the two technological levels. In HT, it was used basic fertilization, two top dressing and supplementary irrigation
Crop Breed. Appl. Biotechnol.. Publicado em: 01/08/2019
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3. Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
Abstract The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in Saccharomyces cerevisiae, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4ncp#2 and chi-NADL/IBSP4ncp#3). The recombinant clones were
Braz. J. Microbiol.. Publicado em: 2016-12
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4. Tomato second cycle hybrids as a source of genetic variability for fruit quality traits
Abstract The objective of this study was to investigate the phenotypic and molecular variability in a F2 generation derived from a SCH (Second Cycle Hybrid) in order to detect QTLs for some fruit traits of tomato. Genome coverage at different levels was achieved by three types of molecular markers (polypeptides, sequence-related amplified polymorphism-SRAP a
Crop Breed. Appl. Biotechnol.. Publicado em: 2016-12
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5. Research on manufacturing text classification based on improved genetic algorithm
ABSTRACT According to the features of texts, a text classification model is proposed. Base on this model, an optimized objective function is designed by utilizing the occurrence frequency of each feature in each category. According to the relation matrix oftext resource and features, an improved genetic algorithm is adopted for solution with integral matrix
Braz. arch. biol. technol.. Publicado em: 20/10/2016
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6. Evidence of ectopic recombination and a repeat-induced point (RIP) mutation in the genome of Sclerotinia sclerotiorum, the agent responsible for white mold
Abstract Two retrotransposons from the superfamilies Copia and Gypsy named as Copia-LTR_SS and Gypsy-LTR_SS, respectively, were identified in the genomic bank of Sclerotinia sclerotiorum. These transposable elements (TEs) contained direct and preserved long terminal repeats (LTR). Domains related to codified regions for gag protein, integrase, reverse transc
Genet. Mol. Biol.. Publicado em: 07/07/2016
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7. Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus
Zucchini yellow mosaic virus (ZYMV) causes substantial economic losses in cucurbit crops. Although ZYMV has been present in Brazil for more than 20 years, there is little information about the biological and molecular characteristics of the isolates found in the country. This study aimed to characterize the experimental hosts, pathotypes and genetic diversit
Sci. agric. (Piracicaba, Braz.). Publicado em: 2015-02
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8. Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast
The Infectious Bursal Disease Virus (IBDV) causes immunosuppression in young chickens. Advances in molecular virology and vaccines for IBDV have been achieved by viral reverse genetics (VRG). VRG for IBDV has undergone changes over time, however all strategies used to generate particles of IBDV involves multiple rounds of amplification and need of in vitro l
Braz. J. Microbiol.. Publicado em: 2014-12
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9. First report of recombination in Potato yellow vein virus (PYVV) in Colombia
Potato yellow vein virus (PYVV) is currently one of the most important viruses that infects potatoes in Colombia and other Andean countries, causing losses in the production of tubers ranging from 25% to 50%. This study analyzed the genetic variability of different viral isolates collected in the department of Nariño, Colombia, through bioinformatics analys
Trop. plant pathol.. Publicado em: 2014-06
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10. Biotechnology of polyketides: new breath of life for the novel antibiotic genetic pathways discovery through metagenomics
The discovery of secondary metabolites produced by microorganisms (e.g., penicillin in 1928) and the beginning of their industrial application (1940) opened new doors to what has been the main medication source for the treatment of infectious diseases and tumors. In fact, approximately 80 years after the discovery of the first antibiotic compound, and despit
Braz. J. Microbiol.. Publicado em: 2013-12
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11. Construction and characterisation of a complete reverse genetics system of dengue virus type 3
Dengue virulence and fitness are important factors that determine disease outcome. However, dengue virus (DENV) molecular biology and pathogenesis are not completely elucidated. New insights on those mechanisms have been facilitated by the development of reverse genetic systems in the past decades. Unfortunately, instability of flavivirus genomes cloned in E
Mem. Inst. Oswaldo Cruz. Publicado em: 11/10/2013
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12. MORPHOLOGICAL AND GENETIC ANALYSIS OF Triplaris guayaquilensis Wedd (POLYGONACEAE): ONE NATIVE TREE OF ECUADOR
ABSTRACT In this paper, we assessed six native populations (55 trees) of Triplaris guayaquilensis Wedd (Fernan Sanchez), one of the major forest species from Ecuador, using morphological and AFLP (Amplified Fragment Length Polymorphisms) data. The populations were collected through two macro-sites (Central coastals: Quevedo, Ventanas, la Guayas; Andean surro
Ciênc. Florest.. Publicado em: 2013-06