Global Proteomic Analysis
Mostrando 1-12 de 19 artigos, teses e dissertações.
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1. Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study
Resumo Este estudo teve como objetivo comparar quantitativamente a diferença da expressão de proteínas na progressão da patogênese pulpar, bem como descrever as funções biológicas das proteínas identificadas no tecido pulpar. As amostras foram obtidas de seis pacientes atendidos na Faculdade de Odontologia de Araçatuba e divididas em três grupos:
Braz. Dent. J.. Publicado em: 2020-06
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2. Abordagem proteômica da interação bactéria-hospedeiro na colibacilose aviária
Escherichia coli patogênicas aviárias (APEC) causam infecções extraintestinais em frangos conhecidas como colibacilose. A APEC MT78, ao contrário de outras linhagens APEC, foi capaz de invadir células não-fagocitárias no modelo de fibroblastos aviários (CEC-32). Considerando que as interações patógeno-hospedeiro envolvem modificações na abundâ
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 2011
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3. System-based proteomic analysis of the interferon response in human liver cells
A global quantitative proteomic analysis in a human hepatoma cell line in the presence and absence of interferon treatment using the isotope coded affinity tag method and tandem mass spectrometry identified several novel regulatory components of the interferon response.
BioMed Central.
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4. Transcriptomic and proteomic analysis of global ischemia and cardioprotection in the rabbit heart
Cardioplegia is used to partially alleviate the effects of surgically induced global ischemia injury; however, the molecular mechanisms involved in this cardioprotection remain to be elucidated. To improve the understanding of the molecular processes modulating the effects of global ischemia and the cardioprotection afforded by cardioplegia, we constructed r
American Physiological Society.
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5. An insight into high-resolution mass-spectrometry data
Mass spectrometry is a powerful tool with much promise in global proteomic studies. The discipline of statistics offers robust methodologies to extract and interpret high-dimensional mass-spectrometry data and will be a valuable contributor to the field. Here, we describe the process by which data are produced, characteristics of the data, and the analytical
Oxford University Press.
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6. A tagging-via-substrate technology for detection and proteomics of farnesylated proteins
A recently developed proteomics strategy, designated tagging-via-substrate (TAS) approach, is described for the detection and proteomic analysis of farnesylated proteins. TAS technology involves metabolic incorporation of a synthetic azido-farnesyl analog and chemoselective derivatization of azido-farnesyl-modified proteins by an elegant version of Staudinge
National Academy of Sciences.
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7. Proteomic analysis of the bacterial cell cycle
A global approach was used to analyze protein synthesis and stability during the cell cycle of the bacterium Caulobacter crescentus. Approximately one-fourth (979) of the estimated C. crescentus gene products were detected by two-dimensional gel electrophoresis, 144 of which showed differential cell cycle expression patterns. Eighty-one of these protein
The National Academy of Sciences.
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8. Enzyme activity profiles of the secreted and membrane proteome that depict cancer cell invasiveness
By primarily measuring changes in transcript and protein abundance, conventional genomics and proteomics methods may fail to detect significant posttranslational events that regulate protein activity and, ultimately, cell behavior. To address these limitations, activity-based proteomic technologies that measure dynamics in protein function on a global scale
National Academy of Sciences.
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9. Proteomic Analysis of Wild-Type Sinorhizobium meliloti Responses to N-Acyl Homoserine Lactone Quorum-Sensing Signals and the Transition to Stationary Phase†
Proteome analysis revealed that two long-chain N-acyl homoserine lactones (AHLs) produced by Sinorhizobium meliloti 1021 induced significant differences in the accumulation of more than 100 polypeptides in early-log-phase cultures of the wild type. Fifty-six of the corresponding proteins have been identified by peptide mass fingerprinting. The proteins affec
American Society for Microbiology.
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10. Using a global proteomic approach to identify proteins affected by estrogen therapy
With the increase in technological capabilities for measuring biological molecules, there is a greater trend to conduct non-biased, discovery-driven studies that collect information on hundreds of molecules in a single study. The hope is that novel findings can be detected within these large datasets. For protein analysis, these non-biased studies are partic
BioMed Central.
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11. Protein expression profiling arrays: tools for the multiplexed high-throughput analysis of proteins
The completion of the human genome sequence has led to a rapid increase in genetic information. The invention of DNA microarrays, which allow for the parallel measurement of thousands of genes on the level of mRNA, has enabled scientists to take a more global view of biological systems. Protein microarrays have a big potential to increase the throughput of p
BioMed Central.
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12. Quantitative proteomic analysis of Myc oncoprotein function
This study applies a new quantitative proteomics technology to the analysis of the function of the Myc oncoprotein in mammalian cells. Employing isotope-coded affinity tag (ICATTM) reagent labeling and tandem mass spectrometry, the global pattern of protein expression in rat myc-null cell
Oxford University Press.