Heterologous Loci
Mostrando 1-12 de 68 artigos, teses e dissertações.
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1. Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)
Abstract The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Arioca
Genet. Mol. Biol.. Publicado em: 21/02/2019
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2. DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
Abstract This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related
Genet. Mol. Biol.. Publicado em: 02/10/2017
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3. Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci
Abstract The pampas deer (Ozotoceros bezoarticus) is close to being classified as ‘globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to
Genet. Mol. Biol.. Publicado em: 02/10/2017
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4. Extra-pair paternity in a Neotropical rainforest songbird, the White-necked Thrush Turdus albicollis (Aves: Turdidae)
ABSTRACT Over the last two decades, several studies have shown that the mating systems of various birds are more complex than previously believed, and paternity tests performed with molecular techniques have proved, for instance, that the commonly observed social monogamy often presents important variations, such as extra-pair paternity. However, data are st
Zoologia (Curitiba). Publicado em: 05/09/2016
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5. Polymorphic heterologous microsatellite loci for population genetics studies of the white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae)
We screened 44 heterologous microsatellites isolated in species of the families Threskiornithidae, Ciconiidae and Ardeidae for their use in a migratory waterbird, the white-faced ibis Plegadis chihi (Vieillot, 1817) (Threskiornithidae). Of the screened loci, 57% amplified successfully and 24% were polymorphic. In two breeding colonies from southern Brazil (N
Genetics and Molecular Biology. Publicado em: 03/02/2012
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6. Carotenoid biosynthesis structural genes in carrot (Daucus carota): isolation, sequence-characterization, single nucleotide polymorphism (SNP) markers and genome mapping.
Carotenoid pigments are important components of the human diet and carrots are the main dietary sources of the vitamin A precursors IX- and /3- carotene. Carotenoids play essential biological roles in plants and the genes coding for the carotenoid pathway enzymes are evolutionarily conserved, but little information exists about these genes for carrot. In thi
Theoretical and Applied Genetics. Publicado em: 2011
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7. Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically des
Genetics and Molecular Biology. Publicado em: 12/03/2010
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8. Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers
In this study, the microsatellite technique was used to evaluate the genetic variability in populations of collared and white-lipped peccaries kept in captivity. Six primers developed for domestic pigs were used and amplified in both species. They revealed the presence of five polymorphic loci and one monomorphic locus. The polymorphic loci included 4 of the
Genetics and Molecular Biology. Publicado em: 22/01/2010
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9. Genetic characterization of 12 heterologous microsatellite markers for the giant tropical tree Cariniana legalis (Lecythidaceae)
Twelve microsatellite loci previously developed in the tropical tree Cariniana estrellensis were genetically characterized in Cariniana legalis. Polymorphisms were assessed in 28 C. legalis individuals found between the Pardo and Mogi-Guaçu River basins in the state of São Paulo, Brazil. Of the 12 loci, 10 were polymorphic and exhibited Mendelian inheritan
Genetics and Molecular Biology. Publicado em: 2010
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10. Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers
In this study, the microsatellite technique was used to evaluate the genetic variability in populations of collared and white-lipped peccaries kept in captivity. Six primers developed for domestic pigs were used and amplified in both species. They revealed the presence of five polymorphic loci and one monomorphic locus. The polymorphic loci included 4 of the
Publicado em: 2010
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11. Caracterização da estrutura genética de populações residentes e migradoras da espécie Salminus brasiliensis da Bacia do rio Mogi-Guaçu
O dourado (Salminus brasiliensis Cuvier, 1816) é um excelente peixe migrador, possuindo grande porte e larga distribuição na América do Sul, com presença nas bacias dos rios Paraná, Uruguai, Prata e Paraguai (Brasil, Argentina, Uruguai e Paraguai), na drenagem da Laguna dos Patos (Rio Grande do Sul, Brasil) e na porção superior do sistema do alto rio
Publicado em: 2010
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12. Genomic resources for the conservation and management of the harpy eagle (Harpia harpyja, Falconiformes, Accipitridae)
We report the characterization and optimization of 45 heterologous microsatellite loci, and the development of a new set of molecular sex markers for the conservation and management of the Neotropical harpy eagle (Harpia harpyja L. 1758). Of the 45 microsatellites tested, 24 were polymorphic, six monomorphic, 10 uncharacterizable due to multiple bands and fi
Genetics and Molecular Biology. Publicado em: 2008