High Throughput Screening
Mostrando 1-12 de 208 artigos, teses e dissertações.
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1. High Throughput Mass Spectrometry Platform for Prospecting High Value Chemicals from Bioconversion of Glycerin
The use of bioprocess to convert low valued biomass or agroindustrial byproducts into high-value chemicals is an emerging area. However, laboratories usually use outdated analytical techniques to identify bioproducts, losing valuable information, such as the identification of unexpected compounds. In this work, we presented a successful systematic and modern
J. Braz. Chem. Soc.. Publicado em: 24/10/2019
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2. Newborn Screening Quality Assurance Program for CFTR Mutation Detection and Gene Sequencing to Identify Cystic Fibrosis
Abstract All newborn screening laboratories in the United States and many worldwide screen for cystic fibrosis. Most laboratories use a second-tier genotyping assay to identify a panel of mutations in the CF transmembrane regulator (CFTR) gene. Centers for Disease Control and Prevention’s Newborn Screening Quality Assurance Program houses a dried blood spo
J. inborn errors metab. screen.. Publicado em: 30/05/2019
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3. Brazilian malaria molecular targets (BraMMT): selected receptors for virtual high-throughput screening experiments
BACKGROUND Owing to increased spending on pharmaceuticals since 2010, discussions about rising costs for the development of new medical technologies have been focused on the pharmaceutical industry. Computational techniques have been developed to reduce costs associated with new drug development. Among these techniques, virtual high-throughput screening (v
Mem. Inst. Oswaldo Cruz. Publicado em: 25/02/2019
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4. The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation
ABSTRACT Screening promising L. thermophiles with high productivity, high efficiency and strong adaptability are very important in lactic acid industry. For this purpose, 80MeV/u carbon ions were applied to irradiate L. thermophiles. After high-throughput screening, a mutant, named SRZ50, was obtained. Different carbon sources or nitrogen sources were provid
Braz. arch. biol. technol.. Publicado em: 14/06/2018
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5. Neonatal screening for four lysosomal storage diseases with a digital microfluidics platform: Initial results in Brazil
Abstract We describe the initial results of a neonatal screening program for four lysosomal storage diseases (MPS I, Pompe, Gaucher and Fabry) using the digital microfluidics methodology. The method successfully identified patients previously diagnosed with these diseases and was used to test dried blood spot samples obtained from 10,527 newborns aged 2 to 1
Genet. Mol. Biol.. Publicado em: 04/06/2018
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6. Busca virtual de compostos bioativos: conceitos e aplicações
The search and use of bioactive compounds for different applications have been investigated, since ancient time. Virtual screening (VS) has emerged as alternative methodological approach to the Combinatory Chemistry and High-Throughput Screening (HTS) in identifying novel drug candidates. In VS, only compounds that are selected applying different computation
Quím. Nova. Publicado em: 2018-06
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7. TSC1 and TSC2 gene mutations and their implications for treatment in Tuberous Sclerosis Complex: a review
Abstract Tuberous sclerosis complex is an autosomal dominant disorder characterized by skin manifestations and formation of multiple tumors in different organs, mainly in the central nervous system. Tuberous sclerosis is caused by the mutation of one of two tumor suppressor genes, TSC1 or TSC2. Currently, the development of novel techniques and great advance
Genet. Mol. Biol.. Publicado em: 20/02/2017
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8. Non-clinical studies required for new drug development - Part I: early in silico and in vitro studies, new target discovery and validation, proof of principles and robustness of animal studies
This review presents a historical overview of drug discovery and the non-clinical stages of the drug development process, from initial target identification and validation, through in silico assays and high throughput screening (HTS), identification of leader molecules and their optimization, the selection of a candidate substance for clinical development, a
Braz J Med Biol Res. Publicado em: 24/10/2016
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9. Theranostic applications of phage display to control leishmaniasis: selection of biomarkers for serodiagnostics, vaccination, and immunotherapy
AbstractPhage display is a high-throughput subtractive proteomic technology used for the generation and screening of large peptide and antibody libraries. It is based on the selection of phage-fused surface-exposed peptides that recognize specific ligands and demonstrate desired functionality for diagnostic and therapeutic purposes. Phage display has provide
Rev. Soc. Bras. Med. Trop.. Publicado em: 2015-08
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10. A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
Braz. J. Microbiol.. Publicado em: 31/03/2015
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11. Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator
A sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of Cerrado and Atlantic Forest located in areas of environmental preservation in the State of Sao Paulo / Brazil, as p
Braz. J. Microbiol.. Publicado em: 10/01/2014
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12. Biotechnology of polyketides: new breath of life for the novel antibiotic genetic pathways discovery through metagenomics
The discovery of secondary metabolites produced by microorganisms (e.g., penicillin in 1928) and the beginning of their industrial application (1940) opened new doors to what has been the main medication source for the treatment of infectious diseases and tumors. In fact, approximately 80 years after the discovery of the first antibiotic compound, and despit
Braz. J. Microbiol.. Publicado em: 2013-12