Homoplasmy Analysis
Mostrando 1-7 de 7 artigos, teses e dissertações.
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1. Análise de homoplasmia de plantas transplastômicas de fumo via PCR em tempo real / Homoplasmy analysis of tobacco transplastomic plants via real-time PCR
A transformação plastidial oferece uma série de vantagens em relação à transformação nuclear, como: altos níveis de expressão de proteínas, capacidade de expressar múltiplos transgenes em operons e contenção gênica pela ausência de transmissão pelo pólen. Devido ao alto número de cópias do genoma plastidial por cloroplasto e ao alto núme
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 10/01/2012
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2. Restriction site heteroplasmy in the mitochondrial DNA of Brycon opalinus (Cuvier, 1819) (Characiformes, Characidae, Bryconiae)
Homoplasmy is a feature usually found in the mtDNA of higher animal taxa. On the other hand, the presence of two classes of mtDNA in the same cell or organism is rare and may appear in length or site variation. Data from mtDNA RFLP analysis of Brycon opalinus populations (Cuvier, 1819; Characiformes, Characidae, Bryconinae) revealed site heteroplasmy from en
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-03
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3. Molecular Analysis of Hypervirulent Somatic Hybrids of the Entomopathogenic Fungi Beauveria bassiana and Beauveria sulfurescens
Protoplast fusion of diauxotrophic mutants of a Beauveria bassiana entomopathogenic strain (Bb28) and a Beauveria sulfurescens toxinogenic strain (Bs2) produced hybrids which were significantly different from the parents in pathogenicity. Some of the hybrids were hypervirulent and killed insects more quickly than the Bb28 strain, probably because these hybri
American Society for Microbiology.
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4. Clonally expanded mtDNA point mutations are abundant in individual cells of human tissues
Using single-cell sequence analysis, we discovered that a high proportion of cells in tissues as diverse as buccal epithelium and heart muscle contain high proportions of clonal mutant mtDNA expanded from single initial mutant mtDNA molecules. We demonstrate that intracellular clonal expansion of somatic point mutations is a common event in normal human tiss
National Academy of Sciences.
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5. Engineering Cytoplasmic Male Sterility via the Chloroplast Genome by Expression of β-Ketothiolase1
While investigating expression of the polydroxybutyrate pathway in transgenic chloroplasts, we addressed the specific role of β-ketothiolase. Therefore, we expressed the phaA gene via the chloroplast genome. Prior attempts to express the phaA gene in transgenic plants were unsuccessful. We studied the effect of light regulation of the phaA gene using the ps
American Society of Plant Biologists.
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6. Strikingly higher frequency in centenarians and twins of mtDNA mutation causing remodeling of replication origin in leukocytes
The presence of a genetic component in longevity is well known. Here, the association of a mtDNA mutation with a prolonged life span in humans was investigated. Large-scale screening of the mtDNA main control region in leukocytes from subjects of an Italian population revealed a homoplasmic C150T transition near an origin of heavy mtDNA-strand synthesis in �
The National Academy of Sciences.
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7. A mitochondrial DNA clone is associated with increased risk for Alzheimer disease.
Severe mitochondrial genetic mutations lead to early degeneration of specific human tissues; milder mitochondrial mutations may cause degeneration at a later point in life. A mutation at position 4336 was reported to occur at increased frequency in individuals with Alzheimer disease (AD) and Parkinson disease [Shoffner, J. M., Brown, M. D., Torroni, A., Lott