Hrp
Mostrando 1-12 de 339 artigos, teses e dissertações.
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1. On the Use of a TiO2 Sol-Gel for Horseradish Peroxidase Enzyme Immobilization for Development of an Amperometric Biosensor
The sol-gel technique has drawn considerable attention in the area of biomolecules immobilization by virtue of its intrinsic properties including chemical inertness, thermal stability, and good biocompatibility. The present work reports the development of a bio-sensing methodology which involves the immobilization of horseradish peroxidase enzyme (HRP) on pe
J. Braz. Chem. Soc.. Publicado em: 2021-06
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2. A Novel Colorimetric Immunoassay of Ultrasensitive Alpha-Fetoprotein Sensing in Magnetic Bead-Based Mimic Enzyme-Chromogenic Substrate System
This work described a simple and feasible colorimetric immunoassay. Using hemin (a horseradish peroxidase (HRP) mimic enzyme) and the mimic enzyme-chromogenic substrate system, it can qualitatively and quantitatively detect a-fetoprotein (AFP) at an ultralow concentration. The glucose oxidase (GOx) catalyzed oxidation of glucose leads to the formation of glu
J. Braz. Chem. Soc.. Publicado em: 2019-04
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3. Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats
In the present study, we successfully demonstrated for the first time the existence of cardiac proteomic differences between non-selectively bred rats with distinct intrinsic exercise capacities. A proteomic approach based on two-dimensional gel electrophoresis coupled to mass spectrometry was used to study the left ventricle (LV) tissue proteome of rats wit
Braz J Med Biol Res. Publicado em: 11/01/2018
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4. Characterization and Evaluation of Silver-Nanoparticle-Incorporated in Composite Graphite Aiming at their Application in Biosensors
Abstract Biosensors based on nanomaterial composites have been investigated for their potential to function as high sensitivity signal response devices. In the present study, we report the fabrication of silver nanoparticles (AgNPs) on a graphite epoxy composite electrode (GEC) and mixed with the polyaniline (a conductive emeraldine salt form polymer) compos
Braz. J. Chem. Eng.. Publicado em: 2017-07
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5. Use of fluorescent oligonucleotide probes for differentiation between Paracoccidioides brasiliensis and Paracoccidioides lutzii in yeast and mycelial phase
BACKGROUND Fluorescence in situ hybridisation (FISH) associated with Tyramide Signal Amplification (TSA) using oligonucleotides labeled with non-radioactive fluorophores is a promising technique for detection and differentiation of fungal species in environmental or clinical samples, being suitable for microorganisms which are difficult or even impossible
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-02
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6. Characterization of the
hrpZ gene fromPseudomonas syringae pv.maculicola M2Pseudomonas syringae pv.maculicola is a natural pathogen of members of the Brassicaceae plant family. Using a transposon-based mutagenesis strategy inPseudomonas syringae pv.maculicola M2 (PsmM2), we conducted a genetic screen to identify mutants that were capable of growing in M9 mediuBraz. J. Microbiol.. Publicado em: 2015-09
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7. Escore de osso trabecular: perspectivas de um método de imagem em aprimoramento
TBS (do inglês, “trabecular bone score”) é um novo método que estima a microarquitetura óssea a partir de uma imagem de densitometria óssea (DXA) da coluna lombar. Apesar de o TBS não ser uma medida física direta da microarquitetura trabecular, ele correlaciona-se com o volume ósseo, densidade da conectividade trabecular, número de trabéculas e
Arq Bras Endocrinol Metab. Publicado em: 2014-07
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8. Chemical, enzymatic and cellular antioxidant activity studies of Agaricus blazei Murrill
Cogumelos apresentam propriedades nutricionais e medicinais bastante empregadas na manutenção da saúde humana e têm sido considerado por pesquisadores como possíveis fontes de seqÜestradores de radicais livres. Neste trabalho, as propriedades antioxidantes de extratos aquosos de Agaricus blazei Murill, produzidos por maceração e decocção, são demo
An. Acad. Bras. Ciênc.. Publicado em: 13/08/2013
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9. Ação do ácido indol-3-acético sobre Staphylococcus aureus / Action of acid indole-3-acetic acido on Staphylococcus aureus
O objetivo foi avaliar a ação do ácido indol-3-acético (AIA) na ausência e presença da peroxidase de raiz forte (HRP) sobre a viabilidade de cepas certificadas de Staphylococcus aureus ATCC 6538 (produtora de biofilme), ATCC 14458 (portadora do gene seb), ATCC 43300 (portadora do gene mecA) e ATCC 29213 (controle). Foi determinada a concentração inib
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/03/2012
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10. An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y
The detection of anti-hepatitis A virus (HAV) antibody levels by diagnostic kits in the convalescent period of disease generally use immunoglobulin G (IgG), which is expensive. An alternative to IgG is immunoglobulin Y (IgY), an immunoglobulin antibody encountered in birds and reptiles. The aim of this study was to develop a competitive immunoenzymatic assay
Mem. Inst. Oswaldo Cruz. Publicado em: 2012-11
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11. Skeletal muscle major histocompatibility complex class I and II expression differences in adult and juvenile dermatomyositis
OBJECTIVE: To analyze major histocompatibility complex expression in the muscle fibers of juvenile and adult dermatomyositis. METHOD: In total, 28 untreated adult dermatomyositis patients, 28 juvenile dermatomyositis patients (Bohan and Peter's criteria) and a control group consisting of four dystrophic and five Pompe's disease patients were analyzed. Routin
Clinics. Publicado em: 2012-08
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12. Anatomia e status da lignificação de plantas de Lophanthera lactescens Ducke crescidas in vitro
Esta pesquisa teve como objetivo avaliar a lignificação em caules jovens de plantas de Lophanthera lactescens Ducke crescidas in vitro. L. lactescens (Malpighiaceae) é uma espécie endêmica da Amazônia Brasileira que, além de propriedades medicinais, apresenta potencial para a indústria de produtos florestais. As plantas foram crescidas in vitro, usan
Brazilian Journal of Biology. Publicado em: 2012-05