Human Chromosomes
Mostrando 1-12 de 1301 artigos, teses e dissertações.
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1. Protocol for chromosome-specific probe construction using PRINS, micromanipulation and DOP-PCR techniques
ABSTRACT Chromosome-specific probes have been widely used in molecular cytogenetics, being obtained with different methods. In this study, a reproducible protocol for construction of chromosome-specific probes is proposed which associates in situ amplification (PRINS), micromanipulation and degenerate oligonucleotide-primed PCR (DOP-PCR). Human lymphocyte cu
An. Acad. Bras. Ciênc.. Publicado em: 07/12/2017
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2. Genotoxic potential of the latex from cotton-leaf physicnut (Jatropha gossypiifolia L.)
Jatropha gossypiifolia L. (Euphorbiaceae), popularly known as cotton-leaf physicnut, is a milky shrub notable for its medicinal properties. The present study aimed to evaluate the toxic, cytotoxic and genotoxic effects of the latex of J. gossypiifolia, using Allium cepa L. as test system. Seeds of A. cepa were exposed to five concentrations of the latex (1.2
Genet. Mol. Biol.. Publicado em: 2015-03
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3. Murine leukemia virus-derived retroviral vector has differential integration patterns in human cell lines used to produce recombinant factor VIII
OBJECTIVE: Nowadays recombinant factor VIII is produced in murine cells including in Chinese hamster ovary (CHO) and baby hamster kidney cells (BHK). Previous studies, using the murine leukemia virus-derived retroviral vector pMFG-FVIII-P140K, modified two recombinant human cell lines, HepG2 and Hek293 to produce recombinant factor VIII. In order to characte
Rev. Bras. Hematol. Hemoter.. Publicado em: 2014-06
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4. Human molecular cytogenetics: from cells to nucleotides
The field of cytogenetics has focused on studying the number, structure, function and origin of chromosomal abnormalities and the evolution of chromosomes. The development of fluorescent molecules that either directly or via an intermediate molecule bind to DNA has led to the development of fluorescent in situ hybridization (FISH), a technology linking cytog
Genet. Mol. Biol.. Publicado em: 2014
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5. Non-linear dynamics of chromosome condensation induced by colcemid
This study investigated the dynamical process of chromosome condensation after colcemid treatment. Two pairs of human chromosomes, #2 and #3, were highlighted for the accurate identification by fluorescence in situ hybridization (FISH). A computerized image analysis system was used to measure the lengths of the two pairs of chromosomes averaged over 50 metap
Braz. arch. biol. technol.. Publicado em: 2013-02
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6. Investigação de alterações na região 22q11 em indivíduos com fissura de palato / Investigation of the alterations in the region 22q11 in individuals with cleft palate
Purpose: To investigate alterations (deletions/duplications) in the 22q11 region in individuals with cleft palate aged 0-2 years, in order to perform early diagnosis of 22q11 deletion syndrome (SD22q11). Local: Genetics and Human Cytogenetics Laboratory, HRAC/USP, Bauru-SP. Methods: We selected 55 individuals with cleft palate, both genders, registered and i
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 08/12/2011
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7. Estudo das alteraÃÃes hematolÃgicas, bioquÃmicas e genotÃxicas induzidas por agrotÃxicos em agricultores do estado do Piauà / STUDY OF POSSIBLE GENETIC CHANGES INDUCED BY PESTICIDES IN FARMERS IN THE STATE OF PIAUÃ
Exposure to pesticides represents a potential risk to human health. In addition to poisoning, these compounds may interact with DNA, causing degenerative diseases, including cancer. The risk assessment for pesticides was conducted in the municipalities of Picos, Piripiri, Barras and Josà de Freitas with farm workers (exposed group) and 55 administrative off
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/09/2011
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8. What influences Hb fetal production in adulthood?
Human hemoglobin genes are located in α and β globin gene clusters in chromosomes 16 and 11, respectively. Different types of hemoglobin are synthesized according to the stage of development with fetal hemoglobin (α2γ2) (Hb F) being the main hemoglobin in the fetal period. After birth, there is a reduction (to about 1%) in Hb F levels and adult hemoglobi
Revista Brasileira de Hematologia e Hemoterapia. Publicado em: 2011-06
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9. Worldwide genetic variation at the 3´-UTR region of the LDLR gene : possible influence of natural selection
The low density lipoprotein receptor gene (LDLR) contains many Alu insertions, and is especially Alu-rich at its 3 -untranslated region (3 -UTR). Previous studies suggested that the LDLR 3 -UTR could regulate gene expression by the stabilization of its mRNA. Given the faster Alu evolutionary rate, and wondering about its consequences in a possibly regulatory
Publicado em: 2011
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10. Genomic instability in human actinic keratosis and squamous cell carcinoma
OBJECTIVE: To compare the repetitive DNA patterns of human actinic keratoses and squamous cell carcinomas to determine the genetic alterations that are associated with malignant transformation. INTRODUCTION: Cancer cells are prone to genomic instability, which is often due to DNA polymerase slippage during the replication of repetitive DNA and to mutations i
Clinics. Publicado em: 2011
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11. Distribution of recombination rates across the chromosome 4 of Arabidopsis thaliana and its association with genomic features / Distribuição de taxas de recombinação ao longo do cromossomo 4 de Arabidopsis thaliana e sua associação com elementos genômicos
Recombination is one of the most important factors in the evolution of genome organization. It provides the links between homologous chromosomes that ensure their proper segregation during the first meiotic division. It is responsible for the creation of novel allele combinations and yields genetic diversity on which evolutionary selection can act. Double-st
Publicado em: 2010
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12. Synteny of human chromosomes 14 and 15 in the platyrrhines (Primates, Platyrrhini)
In order to study the intra- and interspecific variability of the 14/15 association in Platyrrhini, we analyzed 15 species from 13 genera, including species that had not been described yet. The DNA libraries of human chromosomes 14 and 15 were hybridized to metaphases of Alouatta guariba clamitans, A. caraya, A. sara, Ateles paniscus chamek, Lagothrix lagoth
Genetics and Molecular Biology. Publicado em: 28/08/2009