Hybridization Assay
Mostrando 1-12 de 1081 artigos, teses e dissertações.
-
1. Microbial Profile and Endotoxin Levels in Primary Periodontal Lesions with Secondary Endodontic Involvement
Resumo Este estudo foi realizado para investigar o perfil microbiano e os níveis de endotoxina de lesões endoperiodontais de origem periodontal. Amostras periodontais e endodônticas foram obtidas de bolsas periodontais e canais radiculares necróticos de 10 dentes com lesões endoperiodontais. A investigação de 40 espécies bacterianas diferentes foram
Braz. Dent. J.. Publicado em: 22/07/2019
-
2. Correlation Between Volume of Root Canal, Cultivable Bacteria, Bacterial Complexes and Endotoxins in Primary Infection
Resumo Este estudo clínico foi conduzido para correlacionar os níveis de endotoxinas e bactérias cultiváveis encontradas na infecção endodôntica primária (IEP) com o volume do canal radicular determinado pelo uso da Tomografia Computadorizada de Feixe Cônico (TCFC); e avaliar a diversidade bacteriana correlacionada com características clínicas. Vi
Braz. Dent. J.. Publicado em: 04/04/2019
-
3. A novel effect of geraniin on OPG/RANKL signaling in osteoblasts
In this study, the effects of geraniin on osteoprotegerin/receptor activator of nuclear factor-κB ligand(OPG/RANKL) in regulating the proliferation of osteoblasts and suppression of osteoclast-like cells (OLC) in OLC-osteoblast co-cultured system in vitro were investigated. Osteoblasts were cultured and identified with alkaline phosphatase (ALP), gomori sta
Braz. J. Pharm. Sci.. Publicado em: 29/11/2018
-
4. Successful and failed mini-implants: microbiological evaluation and quantification of bacterial endotoxin
Abstract Objectives Using two groups of mini-implants (successful and failed) the objectives of this in vivo study were: to evaluate the microbial contamination by the checkerboard DNA-DNA hybridization technique and to quantify the bacterial endotoxin by the limulus amebocyte lysate assay. Material and Methods The 15 successful and 10 failed mini-implan
J. Appl. Oral Sci.. Publicado em: 10/07/2018
-
5. Influence of Helicobacter pylori culture supernatant on the ecological balance of a dual-species oral biofilm
Abstract Dental caries is a chronic progressive disease occurring in the tooth hard tissue due to multiple factors, in which bacteria are the initial cause. Both Streptococcus mutans and Streptococcus sanguinis are main members of oral biofilm. Helicobacter pylori may also be detected in dental plaque, playing an important role in the development of dental c
J. Appl. Oral Sci.. Publicado em: 22/02/2018
-
6. Influence of co-substrates in the anaerobic degradation of an anionic surfactant
The removal of linear alkylbenzene sulfonate (LAS) was evaluated in a UASB reactor using short-chain alcohols (ethanol and methanol) and complex co-substrate (yeast extract). Using only methanol and ethanol as co-substrates resulted in removal of LAS between 30 and 41%. At the end, addition of a complex substrate (yeast extract) increased the removal of LAS
Braz. J. Chem. Eng.. Publicado em: 2013-09
-
7. Rhinovirus detection using different PCR-based strategies
Human rhinoviruses (HRVs) are the major cause of the common cold. HRVs were recently reclassified into the Enterovirus genus (HEV) in the Picornaviridae family. HRVs and other members of the HEV genus share many common features, including sense RNA genomes and partial nucleotide sequence identity. The aim of this study was to evaluate different HRV detection
Brazilian Journal of Microbiology. Publicado em: 2012-06
-
8. Rapid identification of Mycobacterium tuberculosis complex by a novel hybridization signal amplification method based on self-assembly of DNA-streptavidin nanoparticles
Rapid detection of Mycobacterium tuberculosis complex (MTBC) is a critical step in controlling tuberculosis (TB). In this study, we used IS6110 as the specific identification target to develop a novel hybridization signal amplification method (HSAM) for the rapid and direct detection of MTBC from clinical sputum specimens. This system consists of magnetic be
Brazilian Journal of Microbiology. Publicado em: 2011-09
-
9. Evaluation of INNO-LiPA mycobacteria v2 assay for identification of rapidly growing mycobacteria
A total of 54 rapidly growing mycobacteria (RGM) isolated from patients attended in the two hospitals of Cádiz Bay (Spain) were selected during a seven-year-period (2000-2006) in order to evaluate the INNO-LiPA Mycobacteria v2 assay for mycobacterial identification, based on the reverse hybridization principle. The strains were cultured in Löwenstein-Jense
Brazilian Journal of Microbiology. Publicado em: 2011-09
-
10. Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
We used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 RIF-sensitive and 53 RIF-resistant cultures of Mycobacterium tuberculosis. When compared with the antimicrobial susceptibility test (
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-03
-
11. Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection
Direct smear examination using Ziehl-Neelsen staining for pulmonary tuberculosis (PTB) diagnosis is inexpensive and easy to use, but has the major limitation of low sensitivity. Rapid molecular methods are becoming more widely available in centralized laboratories, but they depend on timely reporting of results and strict quality assurance obtainable only fr
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-03
-
12. Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
We used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 RIF-sensitive and 53 RIF-resistant cultures of Mycobacterium tuberculosis. When compared with the antimicrobial susceptibility test (
Publicado em: 2011