1. Diagnosis of lymphoma in paraffin wax sections by nested PCR and immunohistochemistry.

AIMS: To investigate whether nested polymerase chain reaction (PCR) and immunohistochemistry can be used to diagnose malignant lymphoma. METHODS: Paraffin wax embedded tissue sections from 31 patients with malignant lymphoma were analysed by nested PCR and immunohistochemistry using standard protocols. RESULTS: Nested PCR amplification of 1 pg DNA confirmed

2. Polymerase chain reaction and immunohistochemistry frequently detect occult melanoma cells in regional lymph nodes of melanoma patients.

AIMS: To evaluate immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR) for melanoma associated antigens (MAA) in detecting occult melanoma cells in lymph nodes which were missed on routine pathology. METHODS: Occult melanoma cells were sought in 436 lymph nodes from 32 patients with cutaneous melanoma of the lower extremities by

3. Amplification of the HER2 gene in breast cancers testing 2+ weak positive by HercepTest immunohistochemistry: false‐positive or false‐negative immunohistochemistry?

BMJ Group.

4. Histological diagnosis of cytomegalovirus hepatitis in liver allografts.

AIMS--To determine the incidence of histologically documented cytomegalovirus (CMV) hepatitis following orthotopic liver transplantation (OLT) and to assess the effectiveness of immunohistochemistry and in situ hybridisation (ISH) in detecting CMV. To describe the histological pattern most frequently associated with CMV hepatitis in order to select the biops

5. Evaluation of the Anti-East Asian CagA-Specific Antibody for CagA Phenotyping▿

The determination of the cagA genotype is generally based on sequencing the variable 3′ region of the cagA gene. In a previous study, we successfully generated an anti-East Asian CagA-specific antibody (anti-EAS Ab) immunoreactive only with the East Asian CagA and not with the Western CagA. In a small number of Japanese patients, anti-EAS Ab appeared to be

American Society for Microbiology (ASM).

6. Comparison of reverse transcription polymerase chain reaction, immunohistochemistry, and in situ hybridization for the detection of porcine epidemic diarrhea virus in pigs

Reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry, and in situ hybridization were compared for the detection of porcine epidemic diarrhea virus (PEDV). Fifteen piglets experimentally infected with PEDV were used in the study. In addition, 94 diarrheic piglets submitted to the Department of Veterinary Pathology in Seoul National U

7. Detection by PCR of Neospora caninum in Fetal Tissues from Spontaneous Bovine Abortions

The routine diagnosis of Neospora caninum abortion is based upon histopathologic changes in fetal tissues and identification of tissue parasites by immunohistochemistry. Confirmation of N. caninum infection by immunohistochemistry has low sensitivity. In the present study, we examined the utility of PCR in detecting N. caninum infection in fetal tissues from

American Society for Microbiology.

8. Neoplasms in domestic and wild birds kept in captivity: anatomopatologic and immunohistochemistry evaluation / Neoplasias em aves domésticas e silvestres mantidas em domicílio: avaliação anatomopatológica e imunoistoquímica

Neoplastic diseases are generally seen in some avian species, nevertheless, in Brazil, there are no reports regarding cause and classification of these diseases in birds. As keeping birds as pets is increasing, it is important to upgrade knowledge in this area. This report, made retrospectively in 2000 to 2004 and prospectively in 2005 and 2006 in the Avian

Publicado em: 2008

9. Motor units and immunohistochemistry of cat soleus muscle after long periods of cross-reinnervation

1. Cat soleus (slow twitch) was cross-reinnervated with nerve to flexor hallucis or digitorum longus muscle (fast twitch). More than 3 years later motor unit isometric contractions and muscle immunohistochemistry and histochemistry were investigated. All muscles differed from normal fast or slow muscle.

10. A novel quantitative immunoassay system for p53 using antibodies selected for optimum designation of p53 status.

AIM: To develop a highly sensitive and specific enzyme linked immunosorbent assay (ELISA) system for analysis of p53 protein in cancer lysates. METHODS: The anti-p53 monoclonal antibodies DO7, 1801, BP53.12, and 421, and anti-p53 polyclonal antiserum CM1 were assessed by immunohistochemistry and western blot analysis to identify those most suitable for deter

11. Biomonitoring of microcystin and aflatoxin co-occurrence in aquaculture using immunohistochemistry and genotoxicity assays

This work investigated the effects of co-occurring aflatoxin B1 (AFB1) and microcystin (MC) in aquaculture, using immunohistochemistry and genotoxicity methods. Tilapia (Oreochromis niloticus) were exposed to AFB1 by intraperitoneal and MC (cell extract of Microcystis aeruginosa) by intraperitoneal and immersion routes. The interaction of MC-AFB1 was evaluat

Brazilian Archives of Biology and Technology. Publicado em: 2012-02

12. Late relapse in IgD myeloma: value of immunohistochemistry in trephine biopsy specimens.

IgD myeloma is a rare disorder with a very poor prognosis. An unusual case is reported where prolonged remission was obtained. Immunohistochemical staining of the bone marrow trephine biopsy specimen proved invaluable in documenting early relapse. It is suggested that immunohistochemistry should be adopted more widely in view of the disparities evident in as