Immunomagnetic Separation
Mostrando 1-12 de 86 artigos, teses e dissertações.
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1. Detecção de Campylobacter jejuni em produtos de frango utilizando separação imunomagnética
RESUMO Campylobacter jejuni é o principal causador de gastroenterite bacteriana aguda, e a carne de frango tem se mostrado uma importante fonte de transmissão. Este microrganismo é de difícil isolamento e os métodos convencionais muitas vezes não são eficientes, podendo levar a resultados errôneos. O objetivo deste trabalho foi desenvolver e testar a
Arq. Bras. Med. Vet. Zootec.. Publicado em: 28/10/2019
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2. An evaluation of immunomagnetic separation-real-time PCR (IMS-RTiPCR) combined assay for rapid and specific detection of Escherichia coli O157:H7 in raw milk and ground beef
Abstract The aim of this study is to optimize a rapid and specific method for detection of E. coli O157:H7 (EHEC) from food samples with high background flora using an immunomagnetic separation-real-time polymerase chain reaction assay (IMS-RTiPCR). For this, EHEC cells were recovered from raw milk and ground meat samples that were artificially contaminated
Food Sci. Technol. Publicado em: 06/06/2019
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3. Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR
Abstract The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic separation and IS900 -PCR detection (IMS-IS1 PCR) was employed on milk samples from 265 lactating Holstein cows from Map infected
Braz. J. Microbiol.. Publicado em: 2016-06
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4. Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.
Brazilian Journal of Microbiology. Publicado em: 2012-06
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5. Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts
This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samp
Publicado em: 2010
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6. Methods studies : calcium carbonate floculation and adptation of membrane filtration and immunomagnetic separation techniques for Cryptosporidium and Giardia detection in water samples / Estudo dos metodos : floculação em carbonato de calcio e adaptação das tecnicas de filtração em membrana e separação imunomagnetica para a detecção de Cryptosporidium e Giardia em amostras hidricas
The waterborne transmission route of giardiosis and criptosporidiosis has been considered the major one, causing the occurrence of several gastroenteritis outbreaks in several countries in the last years. Looking to this panorama, was published in Brazil the Governmental Decree 518/04 (Health Department), that recommends the research of these protozoa in wat
Publicado em: 2008
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7. Detection and characterization of epithelial cells in the peripheral blood of patients with breast cancer / Detecção e caracterização de células epiteliais no sangue periférico de pacientes com carcinoma localmente avançado de mama
The aim of this study was to detect and characterize carcinoma cells of patients with breast cancer with clinical stage III analyzing the presence or absence before chemotherapy by enrichment of peripheral blood with super paramagnetic microbeads, using direct Immunomagnetic labeling of intracellular 7/8 cytokeratin. For enrichment, magnetically labeled tumo
Publicado em: 2007
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8. Development of immunological and molecular methods for detection of salmonellae in foods / Desenvolvimento de métodos imunológicos e moleculares para detecção se salmonelas em alimentos
Salmonella is a major cause of food-borne illness all over the world. Conventional cultural method for detection of this bacterium in foods is laborious and time-consuming, requiring at least 4 days to obtain negative results, and 6–7 days for identification and confirmation of positive samples. In this manner, rapid methods, such as enzyme-linked immu
Publicado em: 2005
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9. Improvement of the Immunomagnetic Separation Method Selective for Escherichia coli O157 Strains
Immunomagnetic separation is a useful enrichment method selective for Escherichia coli O157 cells against non-O157 E. coli cells from a preenrichment culture. However, E. coli cells are adsorbed onto a solid surface nonspecifically. With the conventional immunomagnetic separation method, this nonspecific adsorption interfered with immunomagnetic separation.
American Society for Microbiology.
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10. Detection of Listeria monocytogenes in foods by immunomagnetic separation.
Immunomagnetic separation with immunomagnetic beads was used to isolate strains of Listeria monocytogenes both from pure cultures and from heterogeneous suspensions. The monoclonal antibodies used recognized all six strains of serotype 4 but only one of three strains of serotype 1. Coating procedure, incubation time, and number of immunomagnetic beads influe
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11. Detection of Helicobacter pylori in Fecal Samples of Gnotobiotic Mice Infected with H. pylori by an Immunomagnetic-Bead Separation Technique
By an immunomagnetic-bead (IMB) separation technique, isolation of Helicobacter pylori from gastrointestinal and fecal samples of gnotobiotic mice infected with the microorganism was tried. The isolation rate of H. pylori from stomach samples after IMB separation was not higher than that of direct culture of the samples. After IMB separation of feces, H. pyl
American Society for Microbiology.
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12. Evaluation of Immunomagnetic Separation for Recovery of Cryptosporidium parvum and Giardia duodenalis from High-Iron Matrices
In this study we examined the recovery of Cryptosporidium parvum and Giardia duodenalis from matrices containing various concentrations of dissolved iron. The organisms were recovered by using the immunomagnetic separation-immunofluorescent assay method, and the levels of recovery were compared to the dissolved iron concentrations. The levels of recovery of
American Society for Microbiology.