Lipoplexes
Mostrando 1-9 de 9 artigos, teses e dissertações.
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1. Lipid adjuvants as carriers for tuberculosis DNA vaccine / Desenvolvimento de vacina genica veiculada em adjuvantes lipidicos para tratamento da tuberculose
Este trabalho visa o desenvolvimento tecnológico de uma vacina gênica, destinada ao combate à tuberculose, na qual o DNA encontra-se veiculado em lipossomas. Foram enfocados três aspectos principais: 1.A preparação e caracterização de estruturas lipídicas funcionais veiculando o DNA, projetadas para atenderem aos requisitos de imunização contra a
Publicado em: 2006
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2. Adiabatic differential scanning calorimetric study of divalent cation induced DNA - DPPC liposome formulation compacted for gene delivery
Complexes between nucleic acids and phospholipid vesicles have been developed as stable non-viral gene delivery vehicles. Currently employed approach uses positively charged lipid species and a helper zwitterionic lipid, the latter being applied for the stabilization of the whole complex. However, besides problematic steps during their preparation, cationic
Brazilian Archives of Biology and Technology. Publicado em: 2004-11
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3. Trends in lipoplex physical properties dependent on cationic lipid structure, vehicle and complexation procedure do not correlate with biological activity
Using a group of structurally related cytofectins, the effects of different vehicle constituents and mixing techniques on the physical properties and biological activity of lipoplexes were systematically examined. Physical properties were examined using a combination of dye accessibility assays, centrifugation, gel electrophoresis and dynamic light scat
Oxford University Press.
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4. Synergy between cationic lipid and co-lipid determines the macroscopic structure and transfection activity of lipoplexes
The large number of cytofectin and co-lipid combinations currently used for lipoplex-mediated gene delivery reflects the fact that the optimal cytofectin/co-lipid combination varies with the application. The effects of structural changes in both cytofectin and co-lipid were systematically examined to identify structure–activity relationships. Specifically,
Oxford University Press.
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5. Plasmid DNA size does not affect the physicochemical properties of lipoplexes but modulates gene transfer efficiency.
Clinical applications of gene therapy mainly depend on the development of efficient gene transfer vectors. Large DNA molecules can only be transfected into cells by using synthetic vectors such as cationic lipids and polymers. The present investigation was therefore designed to explore the physicochemical properties of cationic lipid-DNA particles, with plas
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6. Negatively charged self-assembling DNA/poloxamine nanospheres for in vivo gene transfer
Over the past decade, numerous nonviral cationic vectors have been synthesized. They share a high density of positive charges and efficiency for gene transfer in vitro. However, their positively charged surface causes instability in body fluids and cytotoxicity, thereby limiting their efficacy in vivo. Therefore, there is a need for developing alternative mo
Oxford University Press.
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7. Structural characteristics of supramolecular assemblies formed by guanidinium-cholesterol reagents for gene transfection
We have recently discovered that cationic cholesterol derivatives characterized by guanidinium polar headgroups are very efficient for gene transfection in vitro and in vivo. In spite of being based on some rationale at the molecular level, the development of these new synthetic vectors was nevertheless empirical. Indeed, the factors and processes underlying
The National Academy of Sciences.
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8. Chemical vectors for gene delivery: a current review on polymers, peptides and lipids containing histidine or imidazole as nucleic acids carriers
DNA/cationic lipid (lipoplexes), DNA/cationic polymer (polyplexes) and DNA/cationic polymer/cationic lipid (lipopolyplexes) electrostatic complexes are proposed as non-viral nucleic acids delivery systems. These DNA-nanoparticles are taken up by the cells through endocytosis processes, but the low capacity of DNA to escape from endosomes is regarded as the m
Blackwell Publishing Ltd.
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9. Peptide-mediated RNA delivery: a novel approach for enhanced transfection of primary and post-mitotic cells
Synthetic vectors were evaluated for their ability to mediate efficient mRNA transfection. Initial results indicated that lipoplexes, but not polyplexes based on polyethylenimine (PEI, 25 and 22 kDa), poly(l-lysine) (PLL, 54 kDa) or dendrimers, mediated efficient translation of mRNA in B16-F10 cells. Significant mRNA transfection was achieved by lipople
Oxford University Press.