Lymphocyte Culture
Mostrando 1-12 de 577 artigos, teses e dissertações.
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1. Opportunistic and non-opportunistic intestinal parasites in HIV/ AIDS patients in relation to their clinical and epidemiological status in a specialized medical service in Goiás, Brazil
ABSTRACT Patients infected with the Human Immunodeficiency Virus (HIV) often have opportunistic infections, among which strongyloidiasis and coccidiosis are the most common parasitic infections that aggravate their health status. This study examined the prevalence of intestinal parasites, particularly of Strongyloides stercoralis and intestinal coccidia in p
Rev. Inst. Med. trop. S. Paulo. Publicado em: 08/03/2018
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2. Genotoxicity Evaluation of Dipotassium -Trioxohydroxytetrafluorotriborate, K2(B3O3F4OH), in Human Lymphocyte Cultures and Mice Reticulocytes
ABSTRACT Genotoxic effects of inorganic molecule dipotassium-trioxohydroxytetrafluorotriborate, K2(B3O3F4OH), a promising new therapeutic for the epidermal changes treatment, have been evaluated. In vitro analysis included evaluation of genotoxic and cytotoxic potential of K2(B3O3F4OH) in concentrations of 0.01, 0.02, 0.05 and 0.06 mg/mL applying cytokinesis
Braz. arch. biol. technol.. Publicado em: 31/10/2016
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3. Methicillin-resistant Staphylococcus aureus in HIV patients: Risk factors associated with colonization and/or infection and methods for characterization of isolates - a systematic review
Staphylococcus aureus is an important cause of infections and HIV-infected individuals are frequently susceptible to this pathogen. The aim of this study was to perform a systematic review to identify both the risk factors associated with colonization/infection by methicillin-resistant S. aureus in HIV patients and the methods used for characterization of is
Clinics. Publicado em: 2014-11
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4. Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed
Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the "river buffaloes" with 2n = 50 and the "swamp buffaloes", 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bear
Genet. Mol. Biol.. Publicado em: 2014-06
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5. Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with chronic Chagas disease after in vitro stimulation with recombinant Trypanosoma cruzi antigens
Introduction CD4+CD25+ T lymphocytes have been implicated in the regulation of host inflammatory response against Trypanosoma cruzi, and may be involved in the clinical course of the disease. Methods Peripheral blood mononuclear cells from patients with chronic Chagas disease were cultured in the presence of T. cruzi recombinant antigens and assayed for l
Rev. Soc. Bras. Med. Trop.. Publicado em: 10/05/2013
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6. A novel noninvasive method to detect rejection after heart transplantation
Prompt and accurate detection of rejection prior to pathological changes after organ transplantation is vital for monitoring rejections. Although biopsy remains the current gold standard for rejection diagnosis, it is an invasive method and cannot be repeated daily. Thus, noninvasive monitoring methods are needed. In this study, by introducing an IL-2 neutra
Braz J Med Biol Res. Publicado em: 2012-12
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7. Efeito da via de sinalizaÃÃo slam sobre cÃlulas t na resposta in vitro à leishmania braziliensis / Immune cells activation is modulated by balancing the signals triggered by a variety of cell surface receptors, including receptor activators, co-stimulating receptors and inhibitory receptors. Receptor-related signaling molecule in lymphocyte activation (SLAM) influences the immune cell activation. In this study we investigated the role of SLAM in immune response of cutaneous leishmaniasis caused by L. braziliensis, as well as if the response of individuals high (HP) or low (LP) IFN-γ producers is modulated by SLAM signaling pathway. Peripheral blood monocuclear cells (PBMC) isolated from 43 health individuals were cultured in vitro with anti-SLAM, rIFN-γ, rIL-12 and phytohemagglutinin in the presence or in the absence of L. braziliensis. It was found that L. braziliensis promoted a significantly reduced SLAM expression in T cells, after 120 h of cultured, possibly indicating activation of this pathway in the initial immune response. SLAM expression behaved differently in HP and LP groups. In LP group, L. braziliensis did not modify SLAM expression in T cells in early immune response. The effect of anti-SLAM on SLAM pathway reduced the expression of this protein in the early stages of the immune response of PBMC stimulated with L. braziliensis. After 120 h the effect of anti-SLAM did not alter CD3+SLAM+ expression in both groups. The proinflammatory cytokines, rIFN-γ and rIL-12, present in the microenvironment with L. braziliensis, reduced SLAM expression only in HP group after 6 h of culture and did not change this response after 120 h. Anti-SLAM at a concentration of 10 μg/ml presented no effect on production of cytokines IFN-γ and IL-13 in both groups, but significantly increased IL-10 production in the HP group. Furthermore anti-SLAM associated with L. braziliensis and rIFN-γ simultaneously did not modify IFN-γ, IL-13 and IL-10 productions. Anti-SLAM associated with L. braziliensis and rIL-12 simultaneously induced an increase of IFN-γ in LP group, and increased IL-13 in HP group. These results suggest that in vitro immune response of PBMC exposed to L. braziliensis, the SLAM signaling pathway acts in modulating Th1 response in HP group and induces a condition of temporary immunosuppression in LP group, not previously described in literature.
Immune cells activation is modulated by balancing the signals triggered by a variety of cell surface receptors, including receptor activators, co-stimulating receptors and inhibitory receptors. Receptor-related signaling molecule in lymphocyte activation (SLAM) influences the immune cell activation. In this study we investigated the role of SLAM in immune re
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/05/2011
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8. EFEITO IN VITRO DO POLIMORFISMO ALA16VAL DO GENE DA SUPERÓXIDO DISMUTASE DEPENDENTE DE MANGANÊS NO METABOLISMO OXIDATIVO DE LINFÓCITOS / IN VITRO EFFECT OF ALA16VAL POLIMORPHISM OG SUPEROXIDE DISMUTASE ENZYME MANGANESE DEPENDENTE IN OXIDATIVE METABOLISM OF LINPHOCYTES
Estudos epidemiológicos sugerem que o desbalanço na atividade de enzimas antioxidantes, geneticamente causado, aumenta o risco de disfunções metabólicas e doenças crônicas não-transmissíveis. Este é o caso do polimorfismo em que ocorre uma troca da Alanina (A) por uma Valina (V) no códon 16 do gene da enzima superóxido dismutase dependente de man
Publicado em: 2010
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9. IdentificaÃÃo e avaliaÃÃo da presenÃa de PapilomavÃrus bovino (BPV) em sangue de bovinos e cultura de linfÃcitos em uma fazenda leiteira do estado de Pernambuco
Bovine papillomaviruses (BPVs), family Papillomaviridae, act as causal agent of warts, bladder and digestive tract cancers, causing significant losses to the cattle economy. Ten types of BPVs have been well characterized (BPV-1-10). This study has as main objective to evaluate sequences of BPV genomes in blood samples collected in a dairy farm in Pernambuco
Publicado em: 2009
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10. Experimental infection with Leishmania chagasi in immunosuppressed Balb/c mice: cytokines and parasite burdens
The immune response in leishmaniasis may result in a polarization of the T lymphocyte subpopulation, altering cell phenotype and resulting in immune protection or disease exacerbation. Leishmania may persist in the body either during asymptomatic infections or after treatment, which represents high risk under immunosuppression. The objective of this study wa
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2009
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11. Avaliação da resposta linfoproliferativa de pacientes com paracoccidioidomicose e indivíduos curados a antígenos de Paracoccidioides brasiliensis: filtrado de cultura, gp43 e gp43 tratada com metaperiodato / Lymphocyte proliferative responses of paracoccidioidomycosis patients and cured individuals to Paracoccidioides brasiliensis antigens: culture filtrate, gp43, and metaperiodated gp43
A Paracoccidioidomicose é micose sistêmica causada pelo fungo Paracoccidioides brasiliensis, endêmico na América Latina, especialmente Brasil. Para melhor avaliarmos a resposta imune celular frente a diferentes componentes do fungo, como gp43, considerada o antígeno imunodominante de P. brasiliensis, filtrado de cultura livre de gp43 (Filtrado), e gp43
Publicado em: 2009
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12. Development of a method for evaluating the induction of specific immunity against tumor cells by monocytes transfection with tumor RNA. / Desenvolvimento de método de avaliação da indução de imunidade específica contra células neoplásicas pela transfecção de monócitos com RNA tumoral.
The cancer immunotherapeutic approach has been increasingly exploited. Among the factors that make it attractive, but also limited, is the use of patient antigenic material. Thus, we propose to establish conditions for extraction and amplification of mRNA tumor, as renewable source of antigens. It is also intended to assess the vaccine effectiveness, using m
Publicado em: 2008