Marker Vaccines
Mostrando 1-12 de 34 artigos, teses e dissertações.
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1. MAPEAMENTO E DELEÇÃO DE EPÍTOPOS LINEARES DE LINFÓCITOS B EM PROTEÍNAS DO VÍRUS DA SÍNDROME RESPIRATÓRIA E REPRODUTIVA DOS SUÍNOS PARA A PRODUÇÃO DE UMA VACINA DIFERENCIAL / MAPPING AND DELETION OF B-CELL LINEAR EPITOPES IN PROTEINS OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS FOR THE PRODUCTION OF A DIFFERENTIAL VACCINE
Porcine reproductive and respiratory syndrome virus (PRRSV) was isolated for the first time in 1991 and since then it has been associated with significant economic losses to the pig industry worldwide. Although vaccination against PRRSV is widely used, an important advance would be the development of marker vaccines allowing serologic discrimination between
Publicado em: 2008
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2. Construction of auxotrophic marker in Mycobacterium bovis BCG, knockout strain for the DPPD and proteomic study of tuberculin / Construção de marcador auxotrófico em Mycobacterium bovis BCG, de uma cepa knockout para DPPD e estudo proteômico da tuberculina
Mycobacterium bovis BCG has the potential to be an effective live vector for multivalent vaccines. However, there are two problems regarding the utilization of recombinant BCG as vaccine. The first one is that most mycobacterial cloning vectors rely on antibiotic resistance gene as selectable marker, which is used for genetic transformation. The second one i
Publicado em: 2008
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3. Development and evaluation of immunization tools based on the adenovirus fiber knob modified with the C4 domain of HIV gp120 glycoprotein. / Desenvolvimento e avaliação de ferramentas de imunização baseadas na região globular da fibra adenoviral modificada com o domínio C4 da glicoproteína gp120 do HIV.
HIV glycoprotein gp120 has conserved domains, one of them being the C4 domain. This region is involved in the recognition of the CD4 marker in target cells and antibodies that recognize this domain can block HIV infection. Previously, the C4 domain was introduced in the adenovirus fiber knob. As the adenovirus fiber stimulates de immune system, we decided to
Publicado em: 2008
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4. Proteína de 18kDa de Mycobacterium leprae: um modelo de carregadora para vacinas de segunda geração / Mycobacterium leprae 18kDa protein: a carrier model to second generation vaccines
The major problem on development of second generation vaccines is the poor response against some immunogens. It has leading an intense research to find other substances non toxic and more efficient on enhancement of the immunological response. The aim of this work was the study of a heat-shock protein from Mycobacterium leprae - 18kDa-hsp - produced in yeast
Publicado em: 1999
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5. Development of a Nonantibiotic Dominant Marker for Positively Selecting Expression Plasmids in Multivalent Salmonella Vaccines
We report the novel application of a herbicide-resistance-based dominant marker for the positive selection of expression plasmids in Salmonella serovar vaccines. The β-lactamase gene of the plasmid pTETnir15, which expresses fragment C of tetanus toxin (TetC), has been replaced with the bar gene marker. The new plasmid pBAT1 can be positively selected in vi
American Society for Microbiology.
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6. Search for Correlates of Protective Immunity Conferred by Anthrax Vaccine
Vaccination by anthrax protective antigen (PA)-based vaccines requires multiple immunization, underlying the need to develop more efficacious vaccines or alternative vaccination regimens. In spite of the vast use of PA-based vaccines, the definition of a marker for protective immunity is still lacking. Here we describe studies designed to help define such ma
American Society for Microbiology.
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7. Protective Immunity Conferred by Attenuated aroA Derivatives of Pasteurella multocida B:2 Strains in a Mouse Model of Hemorrhagic Septicemia
Hemorrhagic septicemia (HS) is a fatal systemic disease of cattle and buffaloes. In South Asia HS is caused by infection with Pasteurella multocida serotype B:2. Some control is achieved with alum-precipitated or oil-adjuvanted killed whole-cell vaccines injected subcutaneously, but these vaccines provide only short-term immunity and require annual administr
American Society for Microbiology.
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8. Diagnostic Approach for Differentiating Infected from Vaccinated Poultry on the Basis of Antibodies to NS1, the Nonstructural Protein of Influenza A Virus
Vaccination programs for the control of avian influenza (AI) in poultry have limitations due to the problem of differentiating between vaccinated and virus-infected birds. We have used NS1, the conserved nonstructural protein of influenza A virus, as a differential diagnostic marker for influenza virus infection. Experimentally infected poultry were evaluate
American Society for Microbiology.
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9. Characteristics of Virulent, Attenuated, and Avirulent Mycoplasma pneumoniae Strains
Homologous pairs of virulent and attenuated or avirulent Mycoplasma pneumoniae strains were derived and compared in an effort to elucidate the mechanisms of virulence. These related strains were found to vary in growth, glycolysis, protein electrophoretic patterns, peroxide formation, morphology, and cytadsorption. Variations in the last two characteristics
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10. Recombinant Rinderpest Vaccines Expressing Membrane-Anchored Proteins as Genetic Markers: Evidence of Exclusion of Marker Protein from the Virus Envelope
Rinderpest virus (RPV) causes a severe disease of cattle resulting in serious economic losses in parts of the developing world. Effective control and elimination of this disease require a genetically marked rinderpest vaccine that allows serological differentiation between animals that have been vaccinated against rinderpest and those which have recovered fr
American Society for Microbiology.
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11. Cationic microparticles: A potent delivery system for DNA vaccines
An approach involving the preparation of biodegradable microparticles with a cationic surface was developed to improve the delivery of adsorbed DNA into antigen-presenting cells after i.m. injection. The microparticles released intact and functional DNA over 2 weeks in vitro. In addition, the microparticles induced higher levels of marker gene expression in
The National Academy of Sciences.
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12. Listeria monocytogenes-Based Antibiotic Resistance Gene-Free Antigen Delivery System Applicable to Other Bacterial Vectors and DNA Vaccines
Plasmids represent a powerful tool to rapidly introduce genes into bacteria and help them reach high expression levels. In vaccine development, with live vaccine vectors, this allows greater flexibility and the ability to induce larger antigen amounts through multiple gene copies. However, plasmid retention often requires antibiotic resistance markers, the p
American Society for Microbiology.