Microbiological Assay Agar Diffusion
Mostrando 1-12 de 22 artigos, teses e dissertações.
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1. Validated microbiological and HPLC methods for the determination of moxifloxacin in pharmaceutical preparations and human plasma
The article presents a comparison between microbiological and high performance liquid chromatographic (HPLC) assays for quantification of moxifloxacin in tablets, ophthalmic solutions and human plasma. The microbiological method employed a cylinder-plate agar diffusion assay using a strain of Esherichia coli ATCC 25922 as the test organism and phosphate buff
Braz. J. Microbiol.. Publicado em: 2012-12
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2. Evaluation of different detection methods of biofilm formation in the clinical isolates
BACKGROUND: Microorganisms growing in a biofilm are associated with chronic and recurrent human infections and are highly resistant to antimicrobial agents. There are various methods to detect biofilm production like Tissue Culture Plate (TCP), Tube method (TM), Congo Red Agar method (CRA), bioluminescent assay, piezoelectric sensors, and fluorescent microsc
Brazilian Journal of Infectious Diseases. Publicado em: 2011-08
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3. Comparison of three experimental designs employed in gentamicin microbiological assay through agar diffusion
A gentamicina é um complexo antibiótico de largo espectro, produzido por actinomicetos do gênero Micromonospora e classificado entre os antibióticos aminoglicosídeos, utilizado no tratamento de infecções graves, devidas a microrganismos Gram-negativos. Alterações da sua atividade antimicrobiana, não demonstradas pelos ensaios químicos, podem ser a
Brazilian Journal of Pharmaceutical Sciences. Publicado em: 2009-09
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4. DESENVOLVIMENTO E VALIDAÇÃO DE METODOLOGIAS ANALÍTICAS PARA DETERMINAÇÃO DO ITRACONAZOL MATÉRIA-PRIMA E CÁPSULAS / DEVELOPMENT AND VALIDATION OF ANALITICAL METHODS FOR DETERMINING ITRACONAZOLE RAW MATERIALS AND CAPSULES
O itraconazol é um antifúngico triazólico de amplo espectro de ação pertencente à classe dos azóis, indicado no tratamento de diferentes tipos de infecções micóticas sistêmicas e superficiais. Seu mecanismo de ação baseia-se na capacidade de inibição da síntese do ergosterol que é um componente de vital importância para a membrana das célu
Publicado em: 2008
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5. Doseamento microbiológico de gentamicina por difusão em agar - proposta de delineamento experimental / Microbiological assay of gentamicin sulfate by agar diffusion - proposal of experimental design
Gentamicin is a broad-spectrum antibiotic complex produced by actinomycetes belonging to Micromonospora genus and classified among aminoglycoside antibiotics, used in the treatment of serious infections derived from Gram-negative microorganisms. Alterations of their antimicrobial activity not shown in chemical assays can be evaluated through microbiological
Publicado em: 2006
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6. Comparative Study of Responses to Neomycins B and C by Microbiological and Gas-Liquid Chromatographic Assay Methods
The relative responses of neomycins B and C have been determined by a microbiological agar-diffusion method, a turbidimetric method, and by a recently developed gas-liquid-chromatographic (GLC) method capable of separating the neomycin isomers. The ratios of response of neomycin C to neomycin B by the individual methods were as follows: agar-diffusion method
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7. Use of Fast Green in Agar-Diffusion Microbiological Assays
Microbiological assay plates containing agar stained with fast green and inoculated with test microorganisms could be readily distinguished from unstained seeded agar plates. The boundaries of zones of growth inhibition were more sharply defined in those plates which contained stained agar.
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8. Disc-Agar Diffusion Microbiological Assay Procedure for Determining Serum and Urine Levels of Sulfacytine and Other Sulfonamides
A reasonably precise, reproducible, and sensitive microbiological procedure for directly assaying sulfacytine and other sulfonamides as antibacterially active drugs has been developed by appropriately modifying the standard disc-agar diffusion technique. Blood and urine levels as low as 3 μg/ml may be determined through the use of a strain of Escherichia co
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9. Microbiological Agar Diffusion Assay for Metronidazole Concentrations in Serum
An agar diffusion method using paper disks on plates seeded with Clostridium perfringens and incubated anaerobically to assay serum levels of metronidazole is described. The lowest serum metronidazole concentration that could be measured was 0.7 μg/ml, and the average error was 4% for 5 μg/ml. This assay could measure metronidazole in the presence of genta
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10. Paper Disk-Agar Diffusion Assay of Penicillin in the Presence of Streptomycin
Microbiological assay of individual antibiotics in mixtures of antibiotics depends on the use of selective inactivation and/or of test bacteria with differential susceptibility. Controlled experiments revealed that streptomycin in concentrations of 20 and 40 μg/ml did not influence a disk diffusion assay of penicillin with Sarcina lutea (ATCC 9341) as the t
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11. Rapid Quantitative Microbiological Assay of Antibiotics and Chemical Preservatives of a Nonantibiotic Nature
A rapid quantitative assay of 14 antibiotics and 6 chemical preservatives by the cylinder-plate diffusion method is described, in which Bacillus stearothermophilus ATCC 12980 was used as the test organism. After 4 hr of incubation at 65 C, the zones of inhibition can be read off easily with the naked eye. The 4-hr assay was compared under identical condition
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12. Inhibitory Effects of Citrates in the Determination of Trace Amounts of Penicillin
In the microbiological assay for the determination of trace amounts of penicillin, it has been shown that sodium citrate and penicillin act synergistically against Sarcina lutea. The inhibitory action of citrate appears to involve a sequestering of divalent cations essential to the growth of the organism as shown by minimal inhibitory concentration and agar