Modified Oligonucleotides
Mostrando 1-12 de 336 artigos, teses e dissertações.
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1. Aptamers as a promising approach for the control of parasitic diseases
ABSTRACT Aptamers are short single-stranded RNA or DNA oligonucleotides that are capable of binding various biological targets with high affinity and specificity. Their identification initially relies on a molecular process named SELEX (Systematic Evolution of Ligands by EXponential enrichment) that has been later modified in order to improve aptamer sensiti
Braz J Infect Dis. Publicado em: 2016-12
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2. Development of sandwich-form biosensor to detect Mycobacterium tuberculosis complex in clinical sputum specimens
Mycobacterium tuberculosis, the causing agent of tuberculosis, comes second only after HIV on the list of infectious agents slaughtering many worldwide. Due to the limitations behind the conventional detection methods, it is therefore critical to develop new sensitive sensing systems capable of quick detection of the infectious agent. In the present study, t
Braz J Infect Dis. Publicado em: 2014-12
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3. Funcionalidade e caracterização das propriedades físico-químicas, biológicas e estruturais da uricase modificada por PEGlação. / Functionality and characterization of physiscal-chemical, biological and structural properties of uricase modified by PEGlation.
A PEGlação é uma bem sucedida estratégia nano-biotecnológica que envolve a ligação covalente do polietilenoglicol (PEG) a uma droga para melhorar sua farmacocinética, farmacodinâmica e perfil imunológico, e portanto, aumentar seu efeito terapêutico. Atualmente, a PEGlação é usada para modificar proteínas, peptídeos, oligonucleotídeos e fragm
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/02/2011
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4. Inoculation and detection of Xanthomonas axonopodis pv. malvacearum in cotton seeds (Gossypium hirsutum L.) / âInoculaÃÃo e detecÃÃo de Xanthomonas axonopodis pv. malvacearum em sementes de algodÃo (Gossypium hirsutum L.)â
ABSTRACT BARBOSA, J. F. Inoculation and detection of Xanthomonas axonopodis pv. malvacearum in cotton seeds (Gossypium hirsutum L.). 2007. 135p. Thesis (Doctorate in Agronomy/Plant Pathology) â Universidade Federal de Lavras, Minas Gerais, Brazil. The objectives in the present study were to develop a technique for artificial inoculation of cotton seeds with
Publicado em: 2007
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5. Design and applications of modified oligonucleotides
Oligonucleotides have a wide range of applications in fields such as biotechnology, molecular biology, diagnosis and therapy. However, the spectrum of uses can be broadened by introducing chemical modifications into their structures. The most prolific field in the search for new oligonucleotide analogs is the antisense strategy, where chemical modifications
Brazilian Journal of Medical and Biological Research. Publicado em: 2003-02
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6. Adutos de DNA gerados por produtos da lipoperoxidação: caracterização, detecção, incorporação em oligonucleotídeos e implicações biológicas / DNA adducts from lipoperoxidation products: characterization, detection, incorporation into oligonucleotides and biological implications
Compostos carcinogênicos estruturalmente diversos ligam-se covalentemente ao DNA formando adutos que, se não reparados, provocam mutações. Inicialmente relacionados apenas a compostos exógenos, atualmente há várias evidências de que compostos gerados endogenamente poderiam modificar o DNA gerando adutos. Dentre os compostos endógenos, os produtos ca
Publicado em: 2001
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7. 2′-O-[2-[(N,N-dimethylamino)oxy]ethyl]-modified oligonucleotides inhibit expression of mRNA in vitro and in vivo
Synthesis and antisense activity of oligonucleotides modified with 2′-O-[2-[(N,N-dimethylamino)oxy] ethyl] (2′-O-DMAOE) are described. The 2′-O-DMAOE-modified oligonucleotides showed superior metabolic stability in mice. The phosphorothioate oligonucleotide ‘gapmers’, with 2′-O-DMAOE- modified nucleoside residues at the ends and 2′-deoxy nucleo
Oxford University Press.
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8. Modification of antisense phosphodiester oligodeoxynucleotides by a 5' cholesteryl moiety increases cellular association and improves efficacy.
Phosphodiester oligodeoxynucleotides bearing a 5' cholesteryl (chol) modification bind to low density lipoprotein (LDL), apparently by partitioning the chol-modified oligonucleotides into the lipid layer. Both HL60 cells and primary mouse spleen T and B cells incubated with fluorescently labeled chol-modified oligonucleotide showed substantially increased ce
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9. Hybridization of 2′-ribose modified mixed-sequence oligonucleotides: thermodynamic and kinetic studies
In this study, we characterize the thermodynamics of hybridization, binding kinetics and conformations of four ribose-modified (2′-fluoro, 2′-O-propyl, 2′-O-methoxyethyl and 2′-O-aminopropyl) decameric mixed-sequence oligonucleotides. Hybridization to the complementary non-modified DNA or RNA decamer was probed by fluorescence and circular-dichr
Oxford University Press.
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10. Inhibition of dengue virus by novel, modified antisense oligonucleotides.
Five different target regions along the length of the dengue virus type 2 genome were compared for inhibition of the virus following intracellular injection of the cognate antisense oligonucleotides and their analogs. Unmodified phosphodiester oligonucleotides as well as the corresponding phosphorothioate oligonucleotides were ineffective in bringing about a
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11. Triplex formation with 2′-O,4′-C-ethylene-bridged nucleic acids (ENA) having C3′-endo conformation at physiological pH
Antigenes, which are substances that inhibit gene expression by binding to double-stranded DNA (dsDNA) in a sequence-specific manner, are currently sought for the treatment of various gene-related diseases. As such antigenes, we developed new nuclease-resistant oligopyrimidine nucleotides that are partially modified with 2′-O,4′-C-ethylene nucleic acids
Oxford University Press.
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12. Epithelial-mesenchymal transformation of embryonic cardiac endothelial cells is inhibited by a modified antisense oligodeoxynucleotide to transforming growth factor beta 3.
During early cardiac development, the progenitor cells of the heart valves and membranous septa undergo an epithelial-mesenchymal transformation. Previous studies have shown that this transformation depends on the activity of a transforming growth factor beta (TGF beta) molecule produced by the heart. In the present study, we have used modified antisense oli