Molecular Weight Fractionation
Mostrando 1-12 de 305 artigos, teses e dissertações.
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1. First serine protease inhibitor isolated from Rhinella schneideri poison
Background Toad secretions are a source of molecules with potential biotechnological application on a wide spectrum of diseases. Toads from theRhinella family have two kinds of poisonous glands, namely granular and mucous glands. Rhinella schneideritoads produce granular secretions that comprise a great number of molecules, including serine proteases inhibit
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 29/09/2015
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2. Flooding tolerance and cell wall alterations in maize mesocotyl during hypoxia.
This research aimed to characterize the tolerance to flooding and alterations in pectic and hemicellulose fractions from mesocotyl of maize tolerant to flooding when submitted to hypoxia. In order to characterize tolerance seeds from maize cultivars Saracura BRS-4154 and BR 107 tolerant and sensitive to low oxygen levels, respectively, were set to germinate.
Pesquisa Agropecuaria Brasileira. Publicado em: 2011
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3. Extraction kinetics and molecular size fractionation of humic substances from two brazilian soils
No presente trabalho foi estudada sistematicamente a extração de substâncias húmicas (SH) de um Oxisol e de um Mollisol do Sul do Brazil, empregando-se soluções de NaOH 0.1 e 0.5 mol L-1 e de Na4P2O7 0.15 mol L-1 a pH 7. A cinética e o rendimento de extração foram monitorados através de espectroscopia UV/Vis. Os ácidos húmicos (AH) e os ácidos f
Publicado em: 2010
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4. Purification and characterization of trypsin inhibitor from Cicer arietinum L. and its efficacy against Helicoverpa armigera
Protease inhibitors in legumes are one of the most promising weapons that confer resistance against insects by inhibiting proteases present in the gut of insect larvae. In the present study, trypsin inhibitor activity was detected in the seed flour extracts of 10 selected varieties of chickpea. The presence of inhibitor was confirmed by dot blot analysis. Al
Brazilian Journal of Plant Physiology. Publicado em: 2008-12
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5. Analise histologica do aparato venenifero e caracterização farmaco-bioquimica da peçonha de Vitalius dubius (Araneae, Theraphosidae) / Histological analysis of the venom apparatus and biochemical and pharmacological characterization of venom from Vitalius dubius (Araneae, Theraphosidae)
Vitalius dubius is a medium-sized, non-aggressive tarantula found in southeastern Brazil. In this work, we examined the histological organization of the venom apparatus of V. dubius and investigated some biochemical and pharmacological properties of this spider´s venom. The venom apparatus consisted of two chelicerae fitted with large fangs for prey immobil
Publicado em: 2008
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6. Subcellular localization of an intracellular serine protease of 68 kDa in Leishmania (Leishmania) amazonensis promastigotes
Here we report the subcellular localization of an intracellular serine protease of 68 kDa in axenic promastigotes of Leishmania (Leishmania) amazonensis, using subcellular fractionation, enzymatic assays, immunoblotting, and immunocytochemistry. All fractions were evaluated by transmission electron microscopy and the serine protease activity was measured dur
Memórias do Instituto Oswaldo Cruz. Publicado em: 2005-07
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7. Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora
The brown algae Padina gymnospora contain different fucans. Powdered algae were submitted to proteolysis with the proteolytic enzyme maxataze. The first extract of the algae was constituted of polysaccharides contaminated with lipids, phenols, etc. Fractionation of the fucans with increasing concentrations of acetone produced fractions with different proport
Brazilian Journal of Medical and Biological Research. Publicado em: 2005-04
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8. Aurapten, a coumarin with growth inhibition against Leishmania major promastigotes
Several natural compounds have been identified for the treatment of leishmaniasis. Among them are some alkaloids, chalcones, lactones, tetralones, and saponins. The new compound reported here, 7-geranyloxycoumarin, called aurapten, belongs to the chemical class of the coumarins and has a molecular weight of 298.37. The compund was extracted from the Rutaceae
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-12
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9. Micrurus spixii (PERUVIAN CORAL SNAKE) VENOM - PRELIMINARY BIOCHEMICAL AND ENZYMATIC CHARACTERIZATION
Micrurus spixii venom was studied after fractionation by Sephadex G-100 SF gel filtration chromatography. Several enzymatic activities and biological effects were investigated in whole venom and fractions. The venom was resolved in four peaks in a range of about 73.2-10.7 kDa molecular weight. Alkaline phosphatase and acetylcholinesterase activities were fou
Journal of Venomous Animals and Toxins. Publicado em: 2002
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10. Equilibrio de osmossedimentação sob-gravidade : polidispersões aquosas de prata
This thesis shows results obtained from theoretical and experimental work on osmosedimentation of silver sols under gravity. The osmosedimentation velocity is shown to be strongly dependent on sol concentration and cell geometry. The results show the existence of a osmosedimentation equilibrium state. In this state, the particle distribution along the cells,
Publicado em: 1983
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11. Submerged Production, Purification, and Crystallization of Acid Carboxypeptidase from Penicillium janthinellum IFO-8070
Penicillium janthinellum IFO-8070 produced an acid carboxypeptidase of molecular weight 51,000 in a liquid medium at 25 C. Maximum enzyme concentration was obtained within 3 to 6 days in a medium containing 2% wheat bran, 1% defatted soybean, and 1% KH2PO4; the initial pH was 2 to 4. When submerged aerobic conditions were used, a 51,000-molecular-weight acid
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12. Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis.
A method for constructing yeast artificial chromosome (YAC) libraries with large insert sizes is reported. High molecular weight human DNA was partially digested with EcoRI and cloned in the vector pYAC4. When unfractionated DNA was used, the mean YAC size was 120kb. Fractionation by pulsed-field gel electrophoresis using a 'waltzer' apparatus to remove smal