Nit Mutants
Mostrando 1-12 de 39 artigos, teses e dissertações.
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1. Vegetative compatibility and heterokaryon formation between different isolates of Colletotrichum lindemuthianum by using the nit mutant system
Colletotrichum lindemuthianum, the causative agent of bean anthracnose, is one of the most common pathogens leading to expressive damage to plants beyond presenting noticeable variability. The knowledge on vegetative compatibility groups (VCGs) is of particular interest in asexual fungi as they subdivide the population in groups that can exchange genetic inf
Brazilian Journal of Microbiology. Publicado em: 2011-03
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2. Variability of Cercospora coffeicola in Minas Gerais State, Brazil, characterized by vegetative compatibility and production of cercosporina / Variabilidade de Cercospora coffeicola em Minas Gerais com base em compatibilidade vegetativa e produção de cercosporina
A cercosporiose é considerada uma doença emergente na cafeicultura. Entretanto, há pouca informação sobre a biologia e ecologia do agente etiológico, Cercospora coffeicola. Para caracterizar a variabilidade de populações do patógeno associadas a cafeeiros nos sistemas convencional e orgânico em três regiões de Minas Gerais, efetuou-se um plano de
Publicado em: 2007
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3. The Isolation and Characterization of Mutants Defective in Nitrate Assimilation in NEUROSPORA CRASSA
The isolation and characterization of mutants altered for nitrate assimilation in Neurospora crassa is described. The mutants isolated can be subdivided into five classes on the basis of growth tests that correspond to the growth patterns of existing mutants at six distinct loci. Mutants with growth characteristics like those of nit-2, nit-3 and nit-6 are as
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4. Nitrate Reduction Mutants of Fusarium Moniliforme (Gibberella Fujikuroi)
Twelve strains of Fusarium moniliforme were examined for their ability to sector spontaneously on toxic chlorate medium. All strains sectored frequently; 91% of over 1200 colonies examined formed chlorate-resistant, mutant sectors. Most of these mutants had lesions in the nitrate reduction pathway and were unable to utilize nitrate (nit mutants). nit mutatio
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5. Arabidopsis mutants resistant to the auxin effects of indole-3-acetonitrile are defective in the nitrilase encoded by the NIT1 gene.
Indole-3-acetonitrile (IAN) is a candidate precursor of the plant growth hormone indole-3-acetic acid (IAA). We demonstrated that IAN has auxinlike effects on Arabidopsis seedlings and that exogenous IAN is converted to IAA in vivo. We isolated mutants with reduced sensitivity to IAN that remained sensitive to IAA. These mutants were recessive and fell into
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6. Isolation of the Chlamydomonas Regulatory Gene Nit2 by Transposon Tagging
Genetic evidence suggests that the NIT2 gene of Chlamydomonas reinhardtii encodes a positive regulator of the nitrate-assimilation pathway. To learn more about the function of the NIT2 gene product, we isolated the gene using a transposon-tagging strategy. A nit2 mutation caused by the insertion of a transposon was identified by testing spontaneous nit2 muta
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7. Purine base transport in nit-2 mutants of Neurospora crassa.
The nit-2 mutants possess general purine transport activity. Reduced hypoxanthine uptake in germinated conidia of these mutants may be a consequence of their defective purine metabolism.
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8. Cloning of Flagellar Genes in Chlamydomonas Reinhardtii by DNA Insertional Mutagenesis
Chlamydomonas is a popular genetic model system for studying many cellular processes. In this report, we describe a new approach to isolate Chlamydomonas genes using the cloned nitrate reductase gene (NIT1) as an insertional mutagen. A linearized plasmid containing the NIT1 gene was introduced into nit1 mutant cells by glass-bead transformation. Of 3000 Nit(
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9. Salt-Sensitive Mutants of Chlamydomonas reinhardtii Isolated after Insertional Tagging.
We describe the isolation of salt-sensitive Chlamydomonas reinhardtii mutants by insertional mutagenesis using the nitrate reductase (Nit1) gene. The plasmid pMN24, containing Nit1, was used for transformation of 305CW15 (nit1 cw15 mt+), and transformants were selected for complementation of the nit- phenotype. From 6875 nit+ colonies, four transformants (S4
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10. Metabolic control and autogenous regulation of nit-3, the nitrate reductase structural gene of Neurospora crassa.
In Neurospora crassa, the expression of nit-3, the structural gene which encodes nitrate reductase, is highly regulated and requires both nitrate induction and nitrogen catabolite derepression. The major nitrogen regulatory gene, nit-2, acts in a positive fashion to turn on the expression of nit-3 and other nitrogen-related genes during nitrogen derepression
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11. Regulation of Glutamate Dehydrogenases in nit-2 and am Mutants of Neurospora crassa
The regulation of the glutamate dehydrogenases was investigated in wild-type Neurospora crassa and two classes of mutants altered in the assimilation of inorganic nitrogen, as either nitrate or ammonium. In the wild-type strain, a high nutrient carbon concentration increased the activity of reduced nicotinamide adenine dinucleotide phosphate (NADPH)-glutamat
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12. Isolation and characterization of conditional-lethal rho mutants of Escherichia coli.
Temperature-sensitive nitA (rho) mutants of E. coli were isolated; one of them was characterized as an amber mutant. These strains show the Nit phenotype (transcription of phage lambda DNA independent of the N gene) at low temperatures and are inviable at high temperatures. The mutated sites appear to be between cya and metE on the chromosome. Temperature-se