Nuclear Localization Signal
Mostrando 1-12 de 687 artigos, teses e dissertações.
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1. Gliclazide reduced oxidative stress, inflammation, and bone loss in an experimental periodontal disease model
Abstract Objective The aim of this study was to evaluate the effects of gliclazide on oxidative stress, inflammation, and bone loss in an experimental periodontal disease model. Material and Methods Male albino Wistar rats were divided into no ligature, ligature, and ligature with 1, 5, and 10 mg/kg gliclazide groups. Maxillae were fixed and scanned usin
J. Appl. Oral Sci.. Publicado em: 21/02/2019
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2. Funções do cálcio nuclear e citosólico na sinalização celular
Nucleoplasmic and cytoplasmic calcium (Ca2+ ) can be regulated independently. The relative contribution of nucleoplasmic and cytoplasmic Ca2+ to biological processes such as gene transcription, cell growth, apoptosis and cell cycle control remain to be -binding proteins fully defined. In order to address this question, we target the Ca2+ parvalbumin (PV) or
Publicado em: 2006
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3. Lipid adjuvants as carriers for tuberculosis DNA vaccine / Desenvolvimento de vacina genica veiculada em adjuvantes lipidicos para tratamento da tuberculose
Este trabalho visa o desenvolvimento tecnológico de uma vacina gênica, destinada ao combate à tuberculose, na qual o DNA encontra-se veiculado em lipossomas. Foram enfocados três aspectos principais: 1.A preparação e caracterização de estruturas lipídicas funcionais veiculando o DNA, projetadas para atenderem aos requisitos de imunização contra a
Publicado em: 2006
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4. Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2
Brazilian Journal of Medical and Biological Research. Publicado em: 2001-09
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5. Homeodomain of yeast repressor alpha 2 contains a nuclear localization signal.
The yeast repressor alpha 2 is shown, by analysis of deletion-bearing alpha 2-beta-galactosidase hybrid proteins, to have two structurally distinct nuclear localization signals. The cellular location of hybrid proteins was determined by indirect immunofluorescence and optical sectioning of whole fixed yeast cells. The two nuclear localization signals are far
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6. Function of two discrete regions is required for nuclear localization of polymerase basic protein 1 of A/WSN/33 influenza virus (H1 N1).
Polymerase basic protein 1 (PB1) of influenza virus (A/WSN/33), when expressed from cloned cDNA in the absence of other viral proteins, accumulates in the nucleus. We have examined the location and nature of the nuclear localization signal of PB1 by using deletion mutants and chimeric constructions with chicken muscle pyruvate kinase, a cytoplasmic protein.
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7. Discrete domains mediate the light-responsive nuclear and cytoplasmic localization of Arabidopsis COP1.
The Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) protein plays a critical role in the repression of photomorphogenesis during Arabidopsis seedling development. We investigated the control of COP1 partitioning between nucleus and cytoplasm, which has been implicated in the regulation of COP1 activity, by using fusion proteins between COP1 and beta-glucur
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8. A hydrophobic protein sequence can override a nuclear localization signal independently of protein context.
Simian virus 40 T antigen is specifically targeted to the nucleus by the signal Pro-Lys-Lys-128-Lys-Arg-Lys-Val. We have previously described the isolation of a simian virus 40 T-antigen mutant, 676FS, which retains a wild-type nuclear localization signal but fails to accumulate properly in the nucleus and interferes with the nuclear localization of heterolo
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9. Signal transduction by steroid hormones: nuclear localization is differentially regulated in estrogen and glucocorticoid receptors.
The glucocorticoid receptor accumulates in nuclei only in the presence of bound hormone, whereas the estrogen receptor has been reported to be constitutively nuclear. To investigate this distinction, we compared the nuclear localization domains of the two receptors and the capacity of their respective hormone-binding regions to regulate nuclear localization
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10. The bovine papillomavirus type 1 E2 transactivator and repressor proteins use different nuclear localization signals.
The E2 gene of bovine papillomavirus type 1 encodes at least three nuclear phosphoproteins that regulate viral transcription and DNA replication. All three proteins have a common C-terminal domain that has DNA-binding and dimerization activities. A basic region in this domain forms an alpha helix which makes direct contact with the DNA target. In this study,
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11. The T-DNA-linked VirD2 protein contains two distinct functional nuclear localization signals.
Agrobacterium tumefaciens causes neoplastic growth in plants by transferring a piece of DNA, called T-DNA, into the nucleus of the plant cell. The virulence protein VirD2 of A. tumefaciens is tightly linked to the T-DNA and is thought to direct it to the plant genome. Here we show that the VirD2 protein contains two nuclear localization signals that are func
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12. A nuclear export signal in the N-terminal regulatory domain of IκBα controls cytoplasmic localization of inactive NF-κB/IκBα complexes
Appropriate subcellular localization is crucial for regulation of NF-κB function. Herein, we show that latent NF-κB complexes can enter and exit the nucleus in preinduction states. The nuclear export inhibitor leptomycin B (LMB) sequestered NF-κB/IκBα complexes in the nucleus. Using deletion and site-directed mutagenesis, we identified a previously unch
The National Academy of Sciences.