Oligonucleotide Probe
Mostrando 1-12 de 921 artigos, teses e dissertações.
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1. Allele and haplotype frequencies of human platelet and leukocyte antigens in platelet donors
RESUMO Objetivo Descrever as frequências alélicas e haplotípicas de genes dos antígenos leucocitários humanos nos loci -A,- B e dos antígenos plaquetários humanos para os sistemas HPA-1 a 9, 11 e 15. Métodos Foram incluídos 867 doadores voluntários, saudáveis, não relacionados, que doaram plaquetas por aférese entre janeiro de 2011 e dezembro
Einstein (São Paulo). Publicado em: 07/02/2019
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2. Protocol for chromosome-specific probe construction using PRINS, micromanipulation and DOP-PCR techniques
ABSTRACT Chromosome-specific probes have been widely used in molecular cytogenetics, being obtained with different methods. In this study, a reproducible protocol for construction of chromosome-specific probes is proposed which associates in situ amplification (PRINS), micromanipulation and degenerate oligonucleotide-primed PCR (DOP-PCR). Human lymphocyte cu
An. Acad. Bras. Ciênc.. Publicado em: 07/12/2017
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3. Use of fluorescent oligonucleotide probes for differentiation between Paracoccidioides brasiliensis and Paracoccidioides lutzii in yeast and mycelial phase
BACKGROUND Fluorescence in situ hybridisation (FISH) associated with Tyramide Signal Amplification (TSA) using oligonucleotides labeled with non-radioactive fluorophores is a promising technique for detection and differentiation of fungal species in environmental or clinical samples, being suitable for microorganisms which are difficult or even impossible
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-02
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4. Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection
Direct smear examination using Ziehl-Neelsen staining for pulmonary tuberculosis (PTB) diagnosis is inexpensive and easy to use, but has the major limitation of low sensitivity. Rapid molecular methods are becoming more widely available in centralized laboratories, but they depend on timely reporting of results and strict quality assurance obtainable only fr
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-03
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5. Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection
Direct smear examination using Ziehl-Neelsen staining for pulmonary tuberculosis (PTB) diagnosis is inexpensive and easy to use, but has the major limitation of low sensitivity. Rapid molecular methods are becoming more widely available in centralized laboratories, but they depend on timely reporting of results and strict quality assurance obtainable only fr
Publicado em: 2011
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6. Polyethylene glycol and polyvinylpirrolidone effect on bacterial rRNA extraction and hybridization from cells exposed to tannins.
In order to detect fluctuations in ruminal microbial populations due to forage tannins using 16S ribosomal RNA (rRNA) probes, recovery of intact rRNA is required. The objective of this work was to evaluate the effect of polyethylene glycol (PEG) and polyvinylpirrolidone (PVP) on extraction of bacterial rRNA, in the presence of tannins from tropical legume fo
Pesquisa Agropecuária Brasileira. Publicado em: 2011
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7. Origem e diferenciação do sistema de cromossomos sexuais ZZ/ZW em Triportheus (Characiformes, Characidae): citogenética, mapeamento dos genes ribossomais e microdissecção cromossômica
Chromosomes of distinct species of Triportheus (Characiformes, Characidae) from different hydrographic basins in Brazil, as well as in Argentina, were analyzed in the present work. Triportheus nematurus, from Piracicaba River (São Paulo State, Brazil), was analyzed for the first time, using conventional cytogenetic techniques, base-specific fluorochromes an
Publicado em: 2007
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8. Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction
American cutaneous leishmaniasis (ACL) is an endemic disease in Northern Argentina. We applied the polymerase chain reaction (PCR) followed by a hybridization labelled probe to 21 paraffin embedded human skin biopsies, already analyzed histologically, from leishmaniasis endemic areas in the province of Tucumán, Argentina. We used primers previously designed
Memórias do Instituto Oswaldo Cruz. Publicado em: 2005-04
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9. Molecular cytogenetic characterization of parental genomes in the partial amphidiploid Triticum aestivum x Thinopyrum ponticum
The wheat line PF 839197 and six hybrid derivatives from a cross between PF 839197 and Thinopyrum ponticum were cytologically characterized by fluorescent in situ hybridization (FISH). Probes for the 5S and 45S rDNA genes (pTa794 and pTa71, respectively), a highly repetitive rye sequence (pSc119.2), the synthetic oligonucleotide (AAG)5, and total genomic DNA
Genetics and Molecular Biology. Publicado em: 2005
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10. Genotyping of group A rotavirus samples from Brazilian children by probe hybridization
The G genotyping of 74 group A rotavirus samples was done by RNA-DNA hybridization (dot-blot) using oligonucleotide probes for the VP7 gene region of the human rotavirus serotypes/genotypes 1, 2, 3 and 4. Thirty-one samples could be genotyped by dot-blot showing the following results: G1 = 16, G4 = 6, G3 = 5, and G2 = 4. The data show circulation of genotype
Brazilian Journal of Medical and Biological Research. Publicado em: 2001-04
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11. probeBase: an online resource for rRNA-targeted oligonucleotide probes
Ribosomal RNA-(rRNA)-targeted oligonucleotide probes are widely used for culture-independent identification of microorganisms in environmental and clinical samples. ProbeBase is a comprehensive database containing more than 700 published rRNA-targeted oligonucleotide probe sequences (status August 2002) with supporting bibliographic and biological annotation
Oxford University Press.
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12. Assessment of the sensitivity and specificity of oligonucleotide (50mer) microarrays
To examine the utility and performance of 50mer oligonucleotide (oligonucleotide probe) microarrays, gene-specific oligonucleotide probes were spotted along with PCR probes onto glass microarrays and the performance of each probe type was evaluated. The specificity of oligonucleotide probes was studied using target RNAs that shared various degrees of sequenc
Oxford University Press.