Oric Vector
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Construção de vetor oriC de Mycoplasma hyopneumoniae uma ferramenta para estudos genéticos do agente da pneumonia enzoótica suína.
Mycoplasma hyopneumoniae é o agente etiológico da pneumonia enzoótica, enfermidade distribuída mundialmente em rebanhos suínos nas fases de crescimento e terminação. A pneumonia enzoótica tem sido considerada uma das mais importantes causas de perdas econômicas na suinocultura mundial. Tendo em vista a disponibilidade dos genomas completos de três
Publicado em: 2008
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2. Development of artificial replicative plasmids for transformation of Mycoplasma pulmonis, M. capricolum and M. mycoïdes subsp. mycoïdes, and disruption of the M. pulmonis hemolysin A gene by homologous recombination / Desenvolvimento de plasmídeos replicativos artificiais para transformação de Mycoplasma pulmonis, M. capricolum e M. mycoïdes subsp. mycoïdes, e dirupção do gene da hemolisina A de M. pulmonis por recombinação homóloga
Mycoplasmas are the smallest microorganisms capable of self replication known to date, responsible for many diseases in man and animals, infecting also plants and insects. They constitute a large group of bacteria, classified in different genera in the class Mollicutes, which main common characteristic, besides the small genome, is the absence of a cell wall
Publicado em: 2002
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3. Incompatibility of plasmids containing the replication origin of the Escherichia coli chromosome.
Plasmids containing the replication origin of the Escherichia coli chromosome (oriC plasmids) are unstable in certain recA strains of E. coli. However, they can be maintained more stably in other recA strains. This stable maintenance has allowed us to study the incompatibility properties of oriC plasmids. We have found that two oriC plasmids are incompatible
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4. Cloning and expression of the Escherichia coli replication origin in a single-stranded DNA phage.
The Escherichia coli DNA replication origin (oriC) and the adjacent asparagine synthetase gene (asnA) have been inserted into the duplex replicative form DNA of the single-stranded phage vector M13Goril. By in vitro recombination, the entire oriC asnA-containing plasmid pJS5 was inserted into M13Gori1 in both possible orientations. Both phage types transduce
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5. Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome.
Sets of overlapping cosmid clones generated by random sampling and fingerprinting methods complement data at pyrB (96.5') and oriC (84') in the published physical map of E. coli. A new cloning strategy using sheared DNA, and a low copy, inducible cosmid vector were used in order to reduce bias in libraries, in conjunction with micro-methods for preparing cos
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6. Replication of M13 oriC bacteriophages in Escherichia coli rep mutant is dependent on the cloned Escherichia coli replication origin.
The involvement of the Escherichia coli rep protein in the replication of M13 chimeric deoxyribonucleic acids (DNAs) carrying the E. coli chromosomal DNA replication origin (oriC) has been examined. Previous studies indicate that the cloning of a 3,550-base-pair sequence of chromosomal DNA containing oriC into an M13 vector allows extensive replication of th
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7. Copy Number Mutations (Cop−) of the Plasmid Containing the Replication Origin (oriC) of the Escherichia coli Chromosome: Lethal Effect of the Cop Region Cloned onto a High-Copy-Number Vector on Host Cells
High-copy-number mutants were isolated from an oriC plasmid. They carried insertion mutations within a region (about 470 base pairs) near the uncB gene. When a segment containing this region was cloned onto a high-copy-number plasmid, such a plasmid could be maintained as an intact form only when it was present in a lower copy number.
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8. Gene Disruption through Homologous Recombination in Spiroplasma citri: an scm1-Disrupted Motility Mutant Is Pathogenic
To determine whether homologous recombination could be used to inactivate selected genes in Spiroplasma citri, plasmid constructs were designed to disrupt the motility gene scm1. An internal scm1 gene fragment was inserted into plasmid pKT1, which replicates in Escherichia coli but not in S. citri, and into the S. citri oriC plasmid pBOT1, which replicates i
American Society for Microbiology.
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9. Integrative and free Spiroplasma citri oriC plasmids: expression of the Spiroplasma phoeniceum spiralin in Spiroplasma citri.
The replication region (oriC) of the Spiroplasma citri chromosome has been recently sequenced, and a 2-kbp DNA fragment was characterized as an autonomously replicating sequence (F. Ye, J. Renaudin, J. M. Bové, and F. Laigret, Curr. Microbiol. 29:23-29, 1994). In the present studies, we have combined this DNA fragment, containing the dnaA gene and the flank
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10. Isolation, characterization, and complementation of a motility mutant of Spiroplasma citri.
The helical mollicute Spiroplasma citri, when growing on low-agar medium, forms fuzzy colonies with occasional surrounding satellite colonies due to the ability of the spiroplasmal cells to move through the agar matrix. In liquid medium, these helical organisms flex, twist, and rotate rapidly. By using Tn4001 insertion mutagenesis, a motility mutant was isol