Phosphoglucose Isomerase
Mostrando 1-12 de 96 artigos, teses e dissertações.
-
1. Carbohydrate metabolism of Xylella fastidiosa: Detection of glycolytic and pentose phosphate pathway enzymes and cloning and expression of the enolase gene
The objective of this work was to assess the functionality of the glycolytic pathways in the bacterium Xylella fastidiosa. To this effect, the enzymes phosphoglucose isomerase, aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase of the glycolytic pathway, and glucose 6-phosphate dehydrogenase of the Entner-Doudoroff pathway were studied, f
Genetics and Molecular Biology. Publicado em: 2003
-
2. Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)
Them aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations from Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52) were prepared for starch gel electrophoresis in order to investigate the genetic variation of the followi
Revista Brasileira de Zoologia. Publicado em: 2001-06
-
3. Expressão, purificação,caracterização e modelagem molecular da enzima phosphoglucose isomerase de Trypanosoma Brucei / Expression, purification characterization and molecular modelling of the phosphoglucose isomerase enzyme from Trypanosoma Brucei
A tripanossomose africana, ou doença do sono, como é popularmente conhecida, atingiu 25 mil pessoas só em 1995. Estima-se que a doença seja responsável pela morte de 10.000 pessoas a cada ano, segundo os dados da Organização Mundial da Saúde. Sua distribuição é exclusivamente africana, devido ao fato de sua transmissão depender da Tsé-Tsé, mosc
Publicado em: 2001
-
4. Caracterização da diversidade genetica em plantas citricas, palmeiras e brassicas atraves de isoenzimas e RAPD
Genetic diversity was studied by means of polymorphism using polyacrilamide gel electrophoresis a DNA polymorphism assay based on the amplification of random DNA segments denominated RAPD, for citrus, palm and cabbage plants. The following citrus species were analyzed: sweet orange (C.sinensis), Hamlin, Natal, Valencia, Pera and Baianinha; clementine, sunki,
Publicado em: 1996
-
5. Immunological similarity between a cyanobacterial enzyme and a nuclear DNA-encoded plastid-specific isozyme from spinach
The immunochemical properties of the plastid and cytosolic isozymes of phosphoglucose isomerase (glucosephosphate isomerase; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) in spinach (Spinacia oleracea) and the single phosphoglucose isomerase enzyme from the cyanobacterium Synechococcus sp. were compared by an application of the enzyme-linked immunosorbe
-
6. Cloning, characterization, and nucleotide sequence analysis of a Zymomonas mobilis phosphoglucose isomerase gene that is subject to carbon source-dependent regulation.
The Zymomonas mobilis gene encoding phosphoglucose isomerase (pgi) was cloned by genetic complementation of an Escherichia coli pgi mutant. An enzyme assay and sodium dodecyl sulfate-polyacrylamide gel electrophoresis confirmed the presence of excess amounts of phosphoglucose isomerase in E. coli clones carrying the Z. mobilis pgi gene. The pgi gene is prese
-
7. Relationship between deficiency of phosphoglucose isomerase in Coprinus macrorhizus and fruiting body formation.
A mutant (pgi) of Coprinus macrorhizus deficient in phosphoglucose isomerase did not grow on fructose and grew poorly on glucose. The pgi mutation inhibited the formation of monokaryotic and dikaryotic fruiting bodies.
-
8. Crystallization and preliminary crystallographic study of the phosphoglucose isomerase from Bacillus subtilis
Phosphoglucose isomerase from B. subtilis has been purified and crystallized. The diffraction quality of the crystal was improved by using a flash-annealing technique and 1.9 Å resolution in-house X-ray data were collected.
International Union of Crystallography.
-
9. Interruption of the phosphoglucose isomerase gene results in glucose auxotrophy in Mycobacterium smegmatis.
Two glycerol utilization mutants of Mycobacterium smegmatis that were unable to utilize most carbon sources except glucose were isolated. Supplementation of these media with small amounts of glucose restored growth in the mutants; these strains are therefore glucose auxotrophs. The mutant phenotype is complemented by the gene encoding phosphoglucose isomeras
-
10. A routine method for the determination of phosphoglucose isomerase activity in body fluid
A simple, rapid colorimetric method for the estimation of phosphoglucose isomerase activity, using the determination of fructose by the method of Roe, Epstein, and Goldstein (1949), is described. An arbitrary fructose standard is advocated and normal values for human serum and cerebrospinal fluid are presented.
-
11. Responses of the Central Metabolism in Escherichia coli to Phosphoglucose Isomerase and Glucose-6-Phosphate Dehydrogenase Knockouts†
The responses of Escherichia coli central carbon metabolism to knockout mutations in phosphoglucose isomerase and glucose-6-phosphate (G6P) dehydrogenase genes were investigated by using glucose- and ammonia-limited chemostats. The metabolic network structures and intracellular carbon fluxes in the wild type and in the knockout mutants were characterized by
American Society for Microbiology.
-
12. Genetic Mapping of Mutations Affecting Phosphoglucose Isomerase and Fructose Diphosphatase in Escherichia coli
The loci on the Escherichia coli genome of mutations affecting phosphoglucose isomerase (pgi) and fructose diphosphatase (fdp) have been determined by conjugation and transduction experiments, chiefly three-factor crosses. The loci for these two constitutive enzymes of central intermediary metabolism lie in the same region of the chromosome, but they are not