Pkac1
Mostrando 1-12 de 12 artigos, teses e dissertações.
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1. System of inorganic phosphate acquisition in the human pathogen fungi Aspergillus fumigatus / Sistema de aquisição de fosfato inorgânico no fungo patogênico humano Aspergillus fumigatus
A percepção e aquisição de nutrientes essenciais do meio ambiente estão associadas com o desenvolvimento do fungo saprófita A. fumigatus em um ambiente hostil. O fosfato inorgânico é um dos nutrientes essenciais para o desenvolvimento deste fungo. Os sistemas para aquisição de fosfato em células eucarióticas têm sido caracterizados como sistema
Publicado em: 2009
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2. Regulation of Protein Kinase A Activity by p90 Ribosomal S6 Kinase 1*
Previously, we reported that the catalytic subunit of cAMP-dependent protein kinase (PKAc) binds to the active p90 ribosomal S6 kinase 1 (RSK1) (Chaturvedi, D., Poppleton, H. M., Stringfield, T., Barbier, A., and Patel, T. B. (2006) Mol. Cell. Biol. 26, 4586–4600). Herein, by overexpressing hemagglutinin-tagged RSK1 fragments in HeLa cells we have identifi
American Society for Biochemistry and Molecular Biology.
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3. The Cyclic AMP-Dependent Protein Kinase A Network Regulates Development and Virulence in Aspergillus fumigatus
Aspergillus fumigatus is an important pathogen of immunocompromised hosts, causing pneumonia and invasive disseminated disease with high mortality. To determine the importance of the cyclic AMP (cAMP) signaling pathway for virulence, the pkaC1 gene encoding a protein kinase A (PKA) catalytic subunit was cloned and characterized. Deletion of pkaC1 led to redu
American Society for Microbiology.
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4. Adeno-Associated Virus Rep78 Protein Interacts with Protein Kinase A and Its Homolog PRKX and Inhibits CREB-Dependent Transcriptional Activation
Adeno-associated virus (AAV) is a human parvovirus of the genus Dependovirus. AAV replication is largely restricted to cells which are coinfected with a helper virus. In the absence of a helper virus, the AAV genome can integrate into a specific chromosomal site where it remains latent until reactivated by superinfection of the host cell with an appropriate
American Society for Microbiology.
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5. Phosphorylation restores activity of L-type calcium channels after rundown in inside-out patches from rabbit cardiac cells.
1. Rundown of L-type calcium channels was studied in inside-out patches made from single isolated rabbit ventricular myocytes, using barium as the charge carrier. 2. In the cell-attached patches single-channel activity was stable for more than 15 min after the patch pipette sealed. beta-Receptor stimulation by isoprenaline caused a characteristic increase in
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6. Assembly of a Ca2+-dependent BK channel signaling complex by binding to β2 adrenergic receptor
Large-conductance voltage and Ca2+-activated potassium channels (BKCa) play a critical role in modulating contractile tone of smooth muscle, and neuronal processes. In most mammalian tissues, activation of β-adrenergic receptors and protein kinase A (PKAc) increases BKCa channel activity, contributing to sympathetic nervous system/hormonal regulation of mem
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7. Functional Interaction between the Coactivator Drosophila CREB-Binding Protein and ASH1, a Member of the Trithorax Group of Chromatin Modifiers
CREB-binding protein (CBP) is a coactivator for multiple transcription factors that transduce a variety of signaling pathways. Current models propose that CBP enhances gene expression by bridging the signal-responsive transcription factors with components of the basal transcriptional machinery and by augmenting the access of transcription factors to DNA thro
American Society for Microbiology.
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8. Evidence that aspartate-85 has a higher pK(a) in all-trans than in 13-cisbacteriorhodopsin.
Three experimental observations indicate that the pK(a) of the purple-to-blue transition (the pK(a) of Asp-85) is higher for all-trans-bR(1) than for 13-cis-bR. First, light adaptation of bacteriorhodopsin (bR) at pHs near the pK(a) of Asp-85 causes an increase in the fraction of the blue membrane present. This transformation is reversible in the dark. Secon
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9. A protein kinase C cDNA without the regulatory domain is active after transfection in vivo in the absence of phorbol ester.
We constructed mutant protein kinase C (PKC) cDNAs which expressed PKC activity in vivo in the absence of phorbol ester activation. A hybrid PKC gene, PKAC, was constructed by substituting the coding region for the N-terminal 253 amino acids of PKC alpha with the N-terminal 17 amino acids of the cyclic AMP-dependent protein kinase catalytic subunit (PKA). A
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10. CulB, a Putative Ubiquitin Ligase Subunit, Regulates Prestalk Cell Differentiation and Morphogenesis in Dictyostelium spp.
Dictyostelium amoebae accomplish a starvation-induced developmental process by aggregating into a mound and forming a single fruiting body with terminally differentiated spores and stalk cells. culB was identified as the gene disrupted in a developmental mutant with an aberrant prestalk cell differentiation phenotype. The culB gene product appears to be a ho
American Society for Microbiology.
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11. CREB Is One Component of the Binding Complex of the Ces-2/E2A-HLF Binding Element and Is an Integral Part of the Interleukin-3 Survival Signal
The Ces-2/E2A-HLF binding element (CBE) is recognized by Caenorhabditis elegans death specification gene product Ces-2 and human acute lymphocytic leukemia oncoprotein E2A-HLF. In an attempt to identify a cellular CBE-binding protein(s) that may be involved in apoptosis regulation in mammals, multiple nuclear binding complexes of CBE were identified in vario
American Society for Microbiology.
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12. cAMP accelerates the decay of stretch-activated inward currents in guinea-pig urinary bladder myocytes.
1. Myocytes from the urinary bladder were stretched longitudinally by 5-20%. At -50 mV, stretch induced whole-cell inward currents (Iin) between -100 and -600 pA. Iin decayed slowly with time to 93 +/- 20% (mean +/- S.E.M., n = 6) of the initial value in 1 min. The mechanisms of this 'adaptation' and its modulation by dibutyryl cAMP (dBcAMP) were analysed wi