Plastids
Mostrando 1-12 de 430 artigos, teses e dissertações.
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1. Revisiting hydropotes of Nymphaeaceae: ultrastructural features associated with glandular functions
ABSTRACT Hydropotes are specialized epidermal structures involved in water and mineral flux into and out of the plant body. We analyzed hydropote morphology of four species of Nymphaeaceae: Nymphaea caerulea, Nymphaea lotus, Nymphaea rubra, and Victoria amazonica. Leaf samples were processed following conventional techniques for plant anatomy and for scannin
Acta Bot. Bras.. Publicado em: 2020-03
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2. Revisiting hydropotes of Nymphaeaceae: ultrastructural features associated with glandular functions
ABSTRACT Hydropotes are specialized epidermal structures involved in water and mineral flux into and out of the plant body. We analyzed hydropote morphology of four species of Nymphaeaceae: Nymphaea caerulea, Nymphaea lotus, Nymphaea rubra, and Victoria amazonica. Leaf samples were processed following conventional techniques for plant anatomy and for scannin
Acta Bot. Bras.. Publicado em: 11/11/2019
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3. Estudo do direcionamento das proteases FtsH plastidiais às membranas dos tilacóides / Study of plastidial FtsH proteases targeting to thylakoid membranes
O complexo FtsH em Arabidopsis, presente nos tilacóides, é formado pelas subunidades FtsH1/FtsH5 (tipo A) e FtsH2/FtsH8 (tipo B). Os tipos A e B apresentam grande identidade em seus domínios maduros, porém nenhuma similaridade é observada na região amino-terminal do peptídeo de trânsito. Em um experimento de importação em cloroplastos isolados, Fts
Publicado em: 2011
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4. Caracterização de plantas transgênicas de tabaco (Nicotiana tabacum L.) que expressam o gene Lhcb1*2 de ervilha quanto aos impactos no desenvolvimento dos cloroplastos e formação do fotossistema II. / Characterization of transgenic tobacco plants (Nicotiana tabacum L.) which express the pea lhcb1*2 gene, upon chloroplast development and assembly of the photossystem II.
A produção vegetal é dependente do processo fotossintético. As técnicas de biologia molecular e transformação genética de plantas trouxeram boas perspectivas para a alteração do metabolismo fotossintético. Plantas transgênicas de tabaco (Nicotiana tabacum, L.) que superexpressam o gene quimérico Lhcb1*2 de ervilha têm sido estudadas por apresen
Publicado em: 2004
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5. Caracterização da localização subcelular da proteína THI1 de Arabidopsis thaliana. / Characterization of the subcellular localization of arabidopsis thaliana thip.
Arabidopsis thaliana thi1 gene product is probably involved in both thiamine biosynthesis as well as protection of organellar DNA from damage. Studies of thiamine biosynthesis in plants suggest a plastid location for the pathway, which is in agreement with the predicted THI1 N-terminal chloroplastic transit peptide (TP). On the other hand, thiamine is synthe
Publicado em: 2002
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6. Nuclear gene iojap conditions a programmed change to ribosome-less plastids in Zea mays
The recessive nuclear gene iojap of Zea mays conditions a permanent, heritable deficiency in the ability of the plastid to differentiate. iojap-affected plastids contain a normal genome as evidenced by comparison of the restriction endonuclease digestion patterns of affected and normal plastids. iojap-affected plastids contain neither detectable ribosomes no
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7. Sucrose Metabolism in Plastids1
The question whether sucrose (Suc) is present inside plastids has been long debated. Low Suc levels were reported to be present inside isolated chloroplasts, but these were argued to be artifacts of the isolation procedures used. We have introduced Suc-metabolizing enzymes in plastids and our experiments suggest substantial Suc entry into plastids. The
American Society of Plant Physiologists.
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8. Preparation and Characterization of Envelope Membranes from Nongreen Plastids
We have developed a reliable procedure for the purification of envelope membranes from cauliflower (Brassica oleracea L.) bud plastids and sycamore (Acer pseudoplatanus L.) cell amyloplasts. After disruption of purified intact plastids, separation of envelope membranes was achieved by centrifugation on a linear sucrose gradient. A membrane fraction, having a
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9. Chlorophyll regulates accumulation of the plastid-encoded chlorophyll apoproteins CP43 and D1 by increasing apoprotein stability.
Chlorophyll apoprotein accumulation in higher plant chloroplasts is controlled by light-dependent chlorophyll formation. Dark-grown plants lack chlorophyll and chlorophyll apoproteins. However, the plastid genes encoding the chlorophyll apoproteins are transcribed; chlorophyll apoprotein mRNA accumulates and associates with polysomes in plastids of dark-grow
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10. Isolation of Plastids from Sunflower Cotyledons during Germination 1
Plastids from cotyledons of sunflower (Helianthus annus L.) seedlings, germinated in the dark or in the light, were isolated by isopycnic sucrose density gradient centrifugation. At all stages of development the whole plastids contained triose phosphate isomerase, NADPH-glyoxylate reductase, and l-dihydroxyphenylalanine oxidase, which were used as marker enz
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11. Isolation of Intact Plastids from a Range of Plant Tissues 1
A technique for the isolation of intact plastids from spinach (Spinacia oleracea) and pea (Pisum sativum) leaves, pea roots and castor bean (Ricinus communis) endosperm is described. This technique involves brief centrifugation of whole homogenates on density gradients. Intact plastids were located in the gradient by assaying for triose phosphate isomerase a
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12. Targeting of the Arabidopsis homomeric acetyl-coenzyme A carboxylase to plastids of rapeseeds.
Acetyl-coenzyme A carboxylase (ACCase) occurs in at least two forms in rapeseed (Brassica napus): a homomeric (HO) and presumably cytosolic isozyme and a heteromeric, plastidial isozyme. We investigated whether the HO-ACCase of Arabidopsis can be targeted to plastids of B. napus seeds. A chloroplast transit peptide and the napin promoter were fused to the Ar