Pollen Analysis Protocol
Mostrando 1-6 de 6 artigos, teses e dissertações.
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1. Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense
Abstract The aim of this study was to observe the genetic relationship between C. frutescens (UENF 1636) and C. chinense (UENF 1785) based on the meiotic behavior, on the meiotic index, and on pollen viability of their F1 hybrids. For meiotic analysis and meiotic index, flower buds were collected and fixed for 24 hours in ethanol: acetic acid (3:1), and then
Crop Breed. Appl. Biotechnol.. Publicado em: 2017-06
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2. Pollen analysis of the post-emergence residue of Melipona (Melikerria) interrupta Latreille (Hymenoptera: Apidae) bred in the central Amazon region
We applied an "adapted" protocol for collecting and processing pollen grains in the pollen analysis of the post-emergence residue of Melipona (Melikerria) interrupta Latreille. The study was conducted at the Sant'Ana honey farm, located on the banks of the Solimões River, in the municipality of Manacapuru, in the state of Amazonas, Brazil, where a colony wa
Acta Bot. Bras.. Publicado em: 2013-12
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3. Estudo interdisciplinar em sedimentos lacustres da região sul do estado de São Paulo. Reconstrução da vegetação e do clima no Quaternário tardio / Interdisciplinary study of lacustrine sediments from Southeastern São Paulo state (Brazil). Late Quaternary vegetation and climate changes
One sediment core collected at the margins of a lake surrounded by Atlantic rainforest vegetation at Parque Estadual do Alto Ribeira (PETAR), southern São Paulo state (Brazil), was analyzed, in order to reconstruct the history of the local vegetation over the last 2500 years. Elemental (Total Organic Carbon TOC and Total Organic Nitrogen), isotopic (C and N
Publicado em: 2009
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4. Specific mitochondrial proteins in pollen: presence of an additional ATP synthase beta subunit.
A protocol was designed to obtain a pure fraction of pollen mitochondria from the diploid species Nicotiana sylvestris, the female parent of the allotetraploid Nicotiana tabacum. Most organelles were morphologically intact and able to perform in organello mitochondrial (mt) protein synthesis. As revealed by two-dimensional protein electrophoresis, numerous q
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5. Developmental control of telomere lengths and telomerase activity in plants.
Telomere lengths and telomerase activity were studied during the development of a model dioecious plant, Melandrium album (syn Silene latifolia). Telomeric DNA consisted of Arabidopsis-type TTTAGGG tandem repeats. The terminal positions of these repeats were confirmed by both Bal31 exonuclease degradation and in situ hybridization. Analysis of terminal restr
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6. Cell type-specific loss of atp6 RNA editing in cytoplasmic male sterile Sorghum bicolor
RNA editing and cytoplasmic male sterility are two important phenomena in higher plant mitochondria. To determine whether correlations might exist between the two, RNA editing in different tissues of Sorghum bicolor was compared employing reverse transcription–PCR and subsequent sequence analysis. In etiolated shoots, RNA editing of transcripts of plant mi
The National Academy of Sciences of the USA.