Post Translational Processing
Mostrando 1-12 de 136 artigos, teses e dissertações.
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1. N-Myristoyltransferases as antileishmanial targets: a piggyback approach with benzoheterocyclic analogues
Leishmaniasis is one of the neglected diseases that remain in need for pharmacological alternatives. In this context, N-Myristoyltransferases (NMT) arise as interesting targets to explore since they are involved in the co/post-translational processing of peptides which are responsible for host cell invasion. Studies that consider these enzymes as targets poi
Braz. J. Pharm. Sci.. Publicado em: 24/10/2019
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2. Functional redundancy in the post-translational processing of the vitellogenin VIT-6 precursor by Caenorhabditis elegans proprotein convertases. / Evidências de redundância funcional entre as pró-hormônio convertases no processamento pós-traducional do precursor da vitelogenina VIT-6 do nematóide Caenorhabditis elegans.
Caenorhabditis elegans possui quatro genes de kpcs (kex2/subtilisin-like proprotein convertases): kpc-1, kpc-2/egl-3, kpc-3/aex-5, kpc-4/bli-4. Em C. elegans, dois dos quatro polipeptídeos de vitelogenina encontrados dentro dos ovócitos, YP115 e YP88, se originam a partir de um precursor polipeptídico (VIT-6) clivado pós-traducionalmente após o motivo R
Publicado em: 2009
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3. Heterologous expression and biochemical characterization of an α1,2-mannosidase encoded by the Candida albicans MNS1 gene
Protein glycosylation pathways, commonly found in fungal pathogens, offer an attractive new area of study for the discovery of antifungal targets. In particular, these post-translational modifications are required for virulence and proper cell wall assembly in Candida albicans, an opportunistic human pathogen. The C. albicans MNS1 gene is predicted to encode
Memórias do Instituto Oswaldo Cruz. Publicado em: 2008-11
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4. Identification of proteins from honeybee venom (Apis mellifera L.) immunoreactives to antivenom serum through a proteomic approach / Identificação das proteínas do veneno de abelhas africanizadas (Apis mellifera L.) imunoreativas ao soro antiveneno por abordagem proteômica
O estudo de venenos de artrópodes é de grande interesse para melhorar os tratamentos contra envenenamentos e oferece uma ótima ferramenta para melhor compreensão dos sistemas nervoso e imunológico, coagulação sanguínea e respostas inflamatórias. As abelhas são um dos animais venenosos mais estudados e a elucidação do seu proteoma é de interesse
Publicado em: 2008
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5. Caracterização da expressão e função de receptores recombinantes do hormônio folículo-estimulante. / Characterization of expression and function of recombinant follicle-stimulating receptors.
The follicle stimulating hormone receptor (FSHR) is a member of a family of Gprotein-coupled receptors that has a large aminoterminal domain, rich in leucine repeats. The FSHR, together with the luteinizing hormone receptor, controls gonadal function in vertebrates. Mutations in the FSHR can alter its binding and signaling properties, and drastically affect
Publicado em: 2006
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6. Exocrine secretion granules contain peptide amidation activity.
Exocrine secretion granules from the rat parotid gland contain a carboxyl-terminal peptide alpha-amidation enzyme resembling closely an enzyme from the pituitary (peptidyl-glycine alpha-amidating monooxygenase) that functions in post-translational processing of secretory polypeptides within neural and endocrine secretion granules. alpha-Amidation is a charac
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7. Post-translational processing of p21ras is two-step and involves carboxyl-methylation and carboxy-terminal proteolysis.
We have studied the post-translational processing of p21ras proteins. The primary translation product pro-p21 is cytosolic and is rapidly converted to a cytosolic form (c-p21) of higher mobility on SDS-PAGE. c-p21 is converted in turn to the membrane-bound mature palmitoylated form (m-p21) of slightly higher mobility. These processing steps are accompanied b
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8. Constitutive transcription and regulation of gene expression in non-photosynthetic plastids of higher plants
The plastid genome in higher plants contains >50 genes for rRNAs, tRNAs and proteins for transcriptional and translational functions, besides the genes encoding photosynthetic proteins. Considering the totipotency of most higher plant cells and the differentiation capacity of plastids, it can be inferred that at least the genes for genetic functions must be
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9. Role of propeptide glycan in post-translational processing and transport of barley lectin to vacuoles in transgenic tobacco.
Mature barley lectin is a dimeric protein composed of two identical 18-kilodalton polypeptides. The subunits of barley lectin are initially synthesized as glycosylated proproteins, which are post-translationally processed to the mature protein preceding or concomitant with deposition of barley lectin in vacuoles. To investigate the functional role of the gly
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10. In Vitro Synthesis and Processing of Tomato Fruit Polygalacturonase 1
The in vitro processing of tomato fruit polygalacturonase (PG) (poly[1,4-α-d-galacturonide]glucanohydrolase, EC 3.2.1.15) was studied. Complete chemical deglycosylation of a mixture of mature, purified PG 2A and PG 2B isozymes (45 and 46 kilodaltons; respectively) with trifluoromethane sulfonic acid yielded a single polypeptide of 42 kilodaltons. Similarly,
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11. γ-Glutamyl Transpeptidase in Transgenic Tobacco Plants. Cellular Localization, Processing, and Biochemical Properties1
γ-Glutamyl transpeptidase (γ-GT) is a ubiquitous enzyme that catalyzes the first step of glutathione (GSH) degradation in the γ-glutamyl cycle in mammals. A cDNA encoding an Arabidopsis homolog for γ-GT was overexpressed in tobacco (Nicotiana tabacum) plants. A high level of the membrane-bound γ-GT activity was localized outside the cell in transgenic p
American Society of Plant Physiologists.
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12. Processing of alkaline phosphatase precursor to the mature enzyme by an Escherichia coli inner membrane preparation.
An inner membrane preparation co-translationally cleaved both the alkaline phosphatase and bacteriophage f1 coat protein precursors to the mature proteins. Post-translational outer membrane proteolysis of pre-alkaline phosphatase generated a protein smaller than the authentic monomer.