Pronuclear
Mostrando 1-12 de 42 artigos, teses e dissertações.
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1. Limitações e controvérsias na determinação do valor preditivo de critérios de morfologia oocitária e embrionária
Com o objetivo de aumentar as taxas de sucesso das pacientes que são submetidas a técnicas de reprodução humana assistida (RHA), numerosos estudos apresentam como foco a identificação do embrião com maior potencial de implantação. Apesar dos avanços tecnológicos significativos da Medicina Reprodutiva baseados no advento da era da genômica, prote�
Rev. Bras. Ginecol. Obstet.. Publicado em: 2015-11
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2. Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration
The objective was to investigate the effects of sex-sorting on early fertilization events in ovum pick up (OPU) derived oocytes fertilized in vitro with frozen-thawed sperm at different co-incubation lengths. Eighty-four OPU sessions were carried out in 18 cyclic, dry and non-stimulated Holstein Friesian and German black pied cows. Ovum pick up oocytes were
R. Bras. Zootec.. Publicado em: 2013-10
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3. Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle.
Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with io
Animal Reproduction Science. Publicado em: 2011
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4. Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments.
In vitro-matured (IVM) bovine oocytes were activated with single and combined treatments of strontium (S), ionomycin (I) and 6-DMAP (D). Using oocytes IVM for 26 h, we observed that activation altered cell cycle kinetics (faster progression, MIII arrest, or direct transition from MII to pronuclear stage) when compared to in vitro fertilization. The effect of
Zygote. Publicado em: 2011
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5. Produção de embriões pró-nucleares em fêmeas doadoras das raças Canindé e Saanen durante um programa de transgênese caprina. / Production of embryos pro-nuclear donor females and Saanen breeds Canindé during a program of transgenic goats.
A produção eficiente e a qualidade dos embriões pró-nucleares são fundamentais para o sucesso de um programa de transgênese caprina. Assim, este trabalho teve por objetivo comparar a produção quanti-qualitativa de embriões pró-nucleares em duas raças caprinas para posterior microinjeção de DNA. Para tanto, foram utilizadas fêmeas das raças Saa
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 17/12/2008
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6. Desenvolvimento embrionário, visualização de pronúcleos e transferência pronuclear após centrifugação de zigotos bovinos em meio com citocalasina.
2007
São Carlos. Publicado em: 2007
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7. Maturação e fecundação "in vitro" de ovócitos de caninos domésticos (Canis familiaris)
Este estudo foi realizado com o objetivo de 1) determinar a eficácia de duas temperaturas (370 C e 390 C) e três intervalos de tempo (48, 72 e 96h) comumente usados na maturação in vitro (MIV) de ovócitos caninos, 2) verificar o efeito de hormônios heterólogos: hormônio folículo estimulante (FSH) 0,5µg/ml, estradiol 20µg/ml e somatotropina humana
Publicado em: 2007
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8. O papel do pronuclear (1976 1986) na formação de recusos humanos para a área nuclear no Brasil
In 1975, an agreement between the governments of Brazil and West-Germany was signed for the construction of nuclear power plants in Brazil as well as the extraction, processing and enrichment of uranium. As the result of the first oil crisis and facing the perspective of a continuous world energy crisis, the Brazilian government decided to turn to nuclear po
Publicado em: 2006
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9. Pronuclear Fusion Failure: An Alternate Conjugational Pathway in Tetrahymena Thermophila, Induced by Vinblastine
Vinblastine is shown to induce pronuclear fusion failure in conjugating Tetrahymena thermophila. In this alternate conjugational pathway gametic pronuclei are exchanged between conjugants but do not fuse. Each pronucleus undergoes one mitotic division to produce a new macro- and micronucleus. Genetic consequences of pronuclear fusion failure include the foll
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10. Involvement of fatty acid pathways and cortical interaction of the pronuclear complex in Caenorhabditis elegans embryonic polarity
BioMed Central.
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11. p53-Dependent S-Phase Damage Checkpoint and Pronuclear Cross Talk in Mouse Zygotes with X-Irradiated Sperm
One difficulty in analyzing the damage response is that the effect of damage itself and that of cellular response are hard to distinguish in irradiated cells. In mouse zygotes, damage can be introduced by irradiated sperm, while damage response can be studied in the unirradiated maternal pronucleus. We have analyzed the p53-dependent damage responses in irra
American Society for Microbiology.
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12. Transgenic mice generated by pronuclear injection of a yeast artificial chromosome.
Transgenic mice have become invaluable for analysing gene function and regulation in vivo. However, the size of constructs injected has been limited by the cloning capacity of conventional vectors, a constraint that could be overcome with yeast artificial chromosomes (YACs). We investigated the feasibility of making transgenic mice with YACs by pronuclear in