Proteolysis Inducing Factor
Mostrando 1-12 de 14 artigos, teses e dissertações.
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1. Reduction of growth and resistance cellular of mammary carcinoma after silencing of gene PIF/DCD (Proteolysis-inducing Factor) by shRNAi. / Redução do crescimento e resistência celular de carcinoma mamário após silenciamento do gene PIF/DCD (Proteolysis-Inducing Factor) via shRNAi.
A expressão do PIF/DCD em tumores tem sido relacionada ao crescimento e resistência à morte celular e à indução de caquexia em camundongos. O objetivo deste estudo foi investigar o papel do PIF/DCD nestes processos. Foram construídos três PIF/DCD-RNAi chamados de IBC-I, II, III e pKLO (controle), compreendendo diferentes áreas do mRNA, geramos clone
Publicado em: 2007
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2. "Expressão gênica do fator de indução de proteólise (PIF) e de sua forma variante (PIF-SV) em células normais e malignas" / Genetic expression of proteolsys-inducing factor (PIF) and its splicing form (PIF-SV) in normal and tumor cells
Proteolysis-inducing factor (PIF) is a 24 kDa glycoprotein identified in plasma of cancer patients and responsible for muscle catabolism associated with the process of cancer cachexia. The present study has investigated, using the RT-PCR, RACE-PCR and Real-time PCR techniques, the presence of PIF messages in different cell types derived from normal and tumor
Publicado em: 2004
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3. Survival from sepsis. The significance of altered protein metabolism regulated by proteolysis inducing factor, the circulating cleavage product of interleukin-1.
Amino acid (AA) arterial blood plasma concentrations, K1 (peripheral production + infusion rates), and central plasma clearance rates (K1 divided by arterial concentration) (CPCR-AA) were measured in 70 seriously septic patients. All of these people were in the "hyperdynamic" state at the time of observation. Thirty-seven recovered and 33 died. In addition,
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4. Posttranslational regulation of insulin-like growth factor binding protein-4 in normal and transformed human fibroblasts. Insulin-like growth factor dependence and biological studies.
Insulin-like growth factor binding protein-4 (IGFBP-4) is a 24-26-kD protein expressed by a variety of cell types in vivo and in vitro. Treatment of normal adult human fibroblasts with 10 nM insulin-like growth factor II (IGF-II) for 24 h resulted in an 85% decrease in endogenous IGFBP-4, as assessed by Western ligand blot analysis of the conditioned medium.
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5. Amino-Terminal Protein Fusions to the TraR Quorum-Sensing Transcription Factor Enhance Protein Stability and Autoinducer-Independent Activity
TraR of Agrobacterium tumefaciens is a member of the LuxR family of quorum-sensing transcription factors and regulates genes required for conjugation and vegetative replication of the tumor-inducing (Ti) plasmid in the presence of the autoinducer 3-oxooctanoyl-homoserine lactone (OOHL). In the absence of OOHL, TraR is rapidly destroyed by proteolysis, sugges
American Society for Microbiology.
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6. Augmentation of NK cell activity by a circulating peptide isolated from the plasma of trauma patients.
Natural killer cell (NK) activity was assessed in patients with nonthermal injury. NK activity was assessed employing a standard 4-hour 51Cr release. Peripheral mononuclear cells from healthy human donors and trauma ward patients served as effector cells at three effector/target ratios. Labeled K562 erythroleukemia cells were used as targets. Multiple diluti
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7. DUB-1, a deubiquitinating enzyme with growth-suppressing activity.
Cytokines regulate cell growth by inducing the expression of specific target genes. Using the differential display method, we have cloned a cytokine-inducible immediate early gene, DUB-1 (for deubiquitinating enzyme). DUB-1 is related to members of the UBP superfamily of deubiquitinating enzymes, which includes the oncoprotein Tre-2. A glutathione S-transfer
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8. Immune evasion by hepatitis C virus NS3/4A protease-mediated cleavage of the Toll-like receptor 3 adaptor protein TRIF
Toll-like receptors (TLRs) bind pathogen-specific ligands early in infection, initiating signaling pathways that lead to expression of multiple protective cellular genes. Many viruses have evolved strategies that block the effector mechanisms induced through these signaling pathways, but viral interference with critical proximal receptor interactions has not
National Academy of Sciences.
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9. Mechanism of tumor promoter inhibition of cellular binding of epidermal growth factor
In previous studies we demonstrated that the tumor-promoting agent 12-O-tetradecanoyl phorbol 13-acetate (TPA) and related macrocyclic diterpenes are potent inhibitors of the binding of epidermal growth factor (EGF) to its cell surface receptors in HeLa cells. The present study explores the specificity and mechanism of this effect. We have found that the sam
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10. CD30-dependent degradation of TRAF2: implications for negative regulation of TRAF signaling and the control of cell survival
CD30 is a cell-surface receptor that can augment lymphocyte activation and survival through its ability to induce the transcription factor NF-κB. CD30, however, has also been implicated in the induction of apoptotic cell death of lymphocytes. Here we show that one of the effects of CD30 signal transduction is to render cells sensitive to apoptosis induced b
Cold Spring Harbor Laboratory Press.
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11. The proteolysis-dependent metaphase to anaphase transition: calcium/calmodulin-dependent protein kinase II mediates onset of anaphase in extracts prepared from unfertilized Xenopus eggs.
It has been shown, using spindles assembled in vitro in extracts containing CSF (the cytostatic factor responsible for arresting unfertilized vertebrate eggs at metaphase), that onset of anaphase requires Ca(2+)-dependent activation of the ubiquitin-dependent proteolytic pathway that destroys both mitotic cyclins and an unknown protein responsible for metaph
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12. Two distinct loci affecting conversion to mucoidy in Pseudomonas aeruginosa in cystic fibrosis encode homologs of the serine protease HtrA.
Conversion to a mucoid, exopolysaccharide alginate-overproducing phenotype in Pseudomonas aeruginosa is associated with chronic respiratory infections in cystic fibrosis. Mucoidy is caused by muc mutations that derepress the alternative sigma factor AlgU, which in turn activates alginate biosynthetic and ancillary regulatory genes. Here we report the molecul