Random Amplified Polymorphic Dna Technique
Mostrando 1-12 de 45 artigos, teses e dissertações.
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1. Molecular identification of three species of Oncideres (Coleoptera: Cerambycidae) using RAPD markers
Abstract: Twig girdlers have a short adult life and a long larval stage (up to one year in some species). This fact, together with lack of morphological traits to identify the species from the larval stage, poses obstacles for the taxonomic identification and characterization of the many twig girdlers found in the wild. To solve this matter, Random Amplified
An. Acad. Bras. Ciênc.. Publicado em: 02/09/2019
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2. Application of RAPD-PCR for Determining the Clonality of Methicillin Resistant Staphylococcus aureus Isolated from Different Hospitals
Randomly amplified polymorphic DNA (RAPD)-PCR was applied with ten random 10-mer primers to examine the molecular diversity among methicillin resistant Staphylococcus aureus (MRSA) strains in the hospitals and to investigate the epidemiological spread of these strains between different hospitals. The main objective of the study was to identify appropriate pr
Braz. arch. biol. technol.. Publicado em: 2014-08
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3. Molecular epidemiology of Candida albicans and Candida glabrata strains isolated from intensive care unit patients in Poland
Over the last decades, Candida spp have been responsible for an increasing number of infections, especially in patients requiring intensive care. Knowledge of local epidemiology and analysis of the spread of these pathogens is important in understanding and controlling their transmission. The aim of this study was to evaluate the genetic diversity of 31 Cand
Mem. Inst. Oswaldo Cruz. Publicado em: 2014-07
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4. Identificação molecular e produção de pectinases por isolados de Cladosporium cladosporioides (Fres.) de Vries / Molecular identification and production pectinase by isolates Cladosporium cladosporioides (Fres.) de Vries
The enzymes market has grown considerably in recent years. These enzymes play an important role in various industries, mainly in the food industry. Special attention has been given to the search for alternatives that reduce production costs and developing processes that will not harm the environment. Disposal of waste is a major problem faced by many agribus
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 05/08/2011
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5. Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis
This study is the first report on genetic differences between isolates of Paracoccidioides brasiliensis from a single patient. We describe a simultaneous infection with genetically distinct isolates of P. brasiliensis in a patient with chronic paracoccidioidomycosis. The clinical isolates were obtained from lesions in different anatomical sites and were char
Memórias do Instituto Oswaldo Cruz. Publicado em: 2010-02
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6. Morphplogical characterization and genetic analysis of Drechslera teres isolates
Net blotch, caused by the phytopathogen Drechslera teres, is a common disease of barley (Hordeum vulgare L) and is responsible for large economic losses in some barley growing areas. In this study the morphology and genetic variability of eight D. teres isolates from different regions of the Brazilian state of Rio Grande do Sul were investigated. Colony morp
Publicado em: 2010
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7. Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
An epidemic of sporotrichosis, a subcutaneous mycosis caused by the fungus Sporothrix schenckii, is ongoing in Rio de Janeiro, Brazil, in which cases of human infection are related to exposure to cats. In an attempt to demonstrate the zoonotic character of this epidemic using molecular methodology, we characterised by DNA-based typing methods 19 human and 25
Memórias do Instituto Oswaldo Cruz. Publicado em: 2009-08
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8. Obtaining molecular markers for prognostic and diagnosis of cutaneous malignant melanoma. / Obtenção de marcadores moleculares para prognóstico e diagnóstico de melanoma cutâneo maligno.
The incidence of malignant skin melanoma (MM) increases around 2,5 a 4% each year in the world. The main risk factors are family history of MM, multiple benign or atypical nevi, and additional factors such as immunossuppression, sun sensibility and UV exposure. Genomic instability is responsible for a collection of mutations that are frequently involved in m
Publicado em: 2009
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9. Assessment of genetic relationship between Klebsiella pneumoniae and Klebsiella oxytoca samples isolated from a dental office
The present study aimed to analyze the genetic similarity between genomic profiles of thirteen Klebsiella oxytoca and seven Klebsiella pneumoniae samples isolated from two different collections carried out in different places of dental offices. Random amplified polymorphic DNA (RAPD) technique and similarity coefficients (calculated by Sorensen-Dice and simp
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2008
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10. Colonização por Candida em indivíduos com candidemia / Candida colonization in individuals with candidemia
In the last two decades, Candida spp. have emerged as important nosocomial pathogens in the world and in Brazil. The identification of the source of infection is important in approaching prevention and control strategies. Strategies for the prevention of endogenous candidiasis may focus, to a certain extent, on methods for reducing mucosal colonization, for
Publicado em: 2008
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11. Genetic diversity of environmental Aspergillus flavus strains in the state of São Paulo, Brazil by random amplified polymorphic DNA
Aspergillus flavus is a very important toxigenic fungus that produces aflatoxins, a group of extremely toxic substances to man and animals. Toxigenic fungi can grow in feed crops, such as maize, peanuts, and soybeans, being thus of high concern for public health. There are toxigenic and non-toxigenic A. flavus variants, but the necessary conditions for expre
Memórias do Instituto Oswaldo Cruz. Publicado em: 16/08/2007
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12. A instabilidade genômica como fator prognóstico e diagnóstico na progressão de queratose actínica para carcinoma espinocelular humano / Genomic instability as a prognostic and diagnostic factor on the progression of human actinic keratosis, to squamous cell carcinoma
Genomic instability has been widely used to characterize cancer cells. Genetic alterations in human actinic keratosis (AK) and squamous cell carcinomas (SCC) were investigated by the random amplified polymorphic DNA (RAPD) method, and microsatellite analysis. DNA was obtained from Brazilian patients diagnosed and treated in the School of Medicine of Universi
Publicado em: 2007