Recombinant Bcg
Mostrando 1-12 de 131 artigos, teses e dissertações.
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1. Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor
The Bacille Calmette-Guérin (BCG) vaccine comprises a family of genetically different strains derived by the loss of genomic regions (RDs) and other mutations. In BCG Moreau, loss of RD16 inactivates rv3405c * , encoding a transcriptional repressor that negatively regulates the expression of Rv3406, an alkyl sulfatase. To evaluate the impact of this loss o
Mem. Inst. Oswaldo Cruz. Publicado em: 11/10/2018
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2. Stable expression of Mycobacterium bovis antigen 85B in auxotrophic M. bovis bacillus Calmette-Guérin
BACKGROUND Bovine tuberculosis (TB) is a zoonotic disease caused by Mycobacterium bovis, responsible for causing major losses in livestock. A cost effective alternative to control the disease could be herd vaccination. The bacillus Calmette-Guérin (BCG) vaccine has a limited efficacy against bovine TB, but can improved by over-expression of protective ant
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-02
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3. The mc2-CMX vaccine induces an enhanced immune response against Mycobacterium tuberculosis compared to Bacillus Calmette-Guérin but with similar lung inflammatory effects
Although the attenuated Mycobacterium bovis Bacillus Calmette-Guérin (BCG) vaccine has been used since 1921, tuberculosis (TB) control still proceeds at a slow pace. The main reason is the variable efficacy of BCG protection against TB among adults, which ranges from 0-80%. Subsequently, the mc2-CMX vaccine was developed with promising results. Nonetheless,
Mem. Inst. Oswaldo Cruz. Publicado em: 2016-04
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4. Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence
When grown in the presence of exogenous collagen I, Mycobacterium bovis BCG was shown to form clumps. Scanning electron microscopy examination of these clumps revealed the presence of collagen fibres cross-linking the bacilli. Since collagen is a major constituent of the eukaryotic extracellular matrices, we assayed BCG cytoadherence in the presence of exoge
Mem. Inst. Oswaldo Cruz. Publicado em: 2012-12
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5. Immunodiagnosis potential of M. tuberculosis CFP-10 for active tuberculosis / Anáilise do potencial diagnóstico do anígeno CFP-10 expresso pelo M. tuberculosis.
The use of specific antigens of M. tuberculosis is extremely important for early diagnosis of tuberculosis and to control the spread of mycobacteria in the population. The CFP-10 is an antigen that is present in M. tuberculosis, but is absent in M. bovis of BCG and environmental mycobacteria. So, the objective of this study was to determine whether the immun
Publicado em: 2010
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6. Aspectos da resposta imune humoral e celular de bovinos naturalmente infectados com mycobacterium bovis e avaliação de vacina de subunidade protéica para tuberculose em camundongos / Cellular and humoral immune response aspects of tuberculosis cattle naturally infected with Mycobacterium bovis and evaluation of a proteic subunit tuberculosis vaccine in mice
Nesta tese foram avaliados vários aspectos da tuberculose bovina e do Mycobacterium bovis. Primeiro caracterizou-se a imunogenicidade do MPT-51, Ag 85 e BCG em ensaio imunoenzimático, onde foram utilizadas 208 amostras de soro de animais TTI positivo e 54 amostras de bovinos negativos. O BCG-M. bovis e o Ag 85 foram fortemente reconhecidos pelos anticorpos
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 05/08/2008
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7. Construction of auxotrophic marker in Mycobacterium bovis BCG, knockout strain for the DPPD and proteomic study of tuberculin / Construção de marcador auxotrófico em Mycobacterium bovis BCG, de uma cepa knockout para DPPD e estudo proteômico da tuberculina
Mycobacterium bovis BCG has the potential to be an effective live vector for multivalent vaccines. However, there are two problems regarding the utilization of recombinant BCG as vaccine. The first one is that most mycobacterial cloning vectors rely on antibiotic resistance gene as selectable marker, which is used for genetic transformation. The second one i
Publicado em: 2008
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8. Modulation of allergic immue responses by recombinant BCG in a murine model of asthma. / Modulação da resposta alérgica por BCG recombinante em modelo murino de asma.
Asma alérgica é uma inflamação pulmonar crônica mediada por células Th2. A Hipótese da Higiene é a teoria aceita para explicar o aumento das alergias nas últimas décadas e preconiza que a menor exposição dos indivíduos a componentes microbianos prejudica a geração mecanismos imunorregulatórios. Nosso estudo abordou a modulação da resposta a
Publicado em: 2008
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9. aspects humoral immune response and cells of cattle naturally infected with Mycobacterium bovis and EVALUATION OF VACCINE FOR TUBERCULOSIS IN protein subunits in mice / aspects humoral immune response and cells of cattle naturally infected with Mycobacterium bovis and EVALUATION OF VACCINE FOR TUBERCULOSIS IN protein subunits in mice / ASPECTOS DA RESPOSTA IMUNE HUMORAL E CELULAR DE BOVINOS NATURALMENTE INFECTADOS COM MYCOBACTERIUM BOVIS E AVALIAÇÃO DE VACINA DE SUBUNIDADE PROTÉICA PARA TUBERCULOSE EM CAMUNDONGOS / ASPECTOS DA RESPOSTA IMUNE HUMORAL E CELULAR DE BOVINOS NATURALMENTE INFECTADOS COM MYCOBACTERIUM BOVIS E AVALIAÇÃO DE VACINA DE SUBUNIDADE PROTÉICA PARA TUBERCULOSE EM CAMUNDONGOS
Several aspects of the bovine tuberculosis were analyzed in this study. The immunogenicity of recombinant MPT-51 (rMPT-51), Ag-85 and M. bovis-BCG were characterized in an immunosorbent assay, where 208 serum samples from positive intradermal tuberculin test (ITT) animals and 54 serum samples from ITT negative animals where analyzed. M. bovis-BCG and Ag-85 w
Publicado em: 2008
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10. ASPECTOS DA RESPOSTA IMUNE HUMORAL E CELULAR DE BOVINOS NATURALMENTE INFECTADOS COM MYCOBACTERIUM BOVIS E AVALIAÇÃO DE VACINA DE SUBUNIDADE PROTÉICA PARA TUBERCULOSE EM CAMUNDONGOS / aspects humoral immune response and cells of cattle naturally infected with Mycobacterium bovis and EVALUATION OF VACCINE FOR TUBERCULOSIS IN protein subunits in mice / aspects humoral immune response and cells of cattle naturally infected with Mycobacterium bovis and EVALUATION OF VACCINE FOR TUBERCULOSIS IN protein subunits in mice / ASPECTOS DA RESPOSTA IMUNE HUMORAL E CELULAR DE BOVINOS NATURALMENTE INFECTADOS COM MYCOBACTERIUM BOVIS E AVALIAÇÃO DE VACINA DE SUBUNIDADE PROTÉICA PARA TUBERCULOSE EM CAMUNDONGOS
Several aspects of the bovine tuberculosis were analyzed in this study. The immunogenicity of recombinant MPT-51 (rMPT-51), Ag-85 and M. bovis-BCG were characterized in an immunosorbent assay, where 208 serum samples from positive intradermal tuberculin test (ITT) animals and 54 serum samples from ITT negative animals where analyzed. M. bovis-BCG and Ag-85 w
Publicado em: 2008
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11. Avaliação do bacilo de Calmette-Guérin recombinante expressando o antígeno S1PT no tratamento do carcinoma urotelial de bexiga em modelo experimental / Evaluation of recombinant bacillus Calmette-Guérin expressing S1PT in the treatment of urothelial bladder carcinoma in an experimental model
Introdução: A imunoterapia intravesical com o bacilo de Calmette-Guérin (BCG) é o tratamento adjuvante de escolha no câncer superficial de bexiga. Recentemente, os estudos do mecanismo imunoterápico do BCG têm permitido identificar as reações imunológicas e os genes associados ao efeito antitumoral, possibilitando a produção de vacinas recombinan
Publicado em: 2008
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12. Caracterização da resposta imune humoral em trabalhadores da área da saúde frente a antígenos recombinantes de Mycobacterium tuberculosis / Characterization of humoral immune response of health care workers against recombinant antigens from Mycobacterium tuberculosis
Tuberculosis (TB), one of the oldest disease in the world, is until our days a public health concern. Its believed that one third of the worlds population is latently infected. Brazil is an endemic area for TB and its incidence in 2005 was about 60 cases per 100.000 inhabitants. One of the most important challenge in TB control is the identification of indiv
Publicado em: 2007