Sedimentation Coefficient And Subunits
Mostrando 1-12 de 41 artigos, teses e dissertações.
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1. Estudo da hemoglobina extracelular gigante de Glossoscolex paulistus (HbGp) por ultracentrifugação analítica e fluorescência em função do pH / Studeis of the giant extracellular hemoglobin of Glossoscolex paulistus (HbGp) by analytical ultracentrifugation and fluorencence as a function of pH
A hemoglobina extracelular gigante do anelídeo Glossoscolex paulistus (HbGp) é homológa à hemoglobina da Lumbricus terrestris (HbLt). Baseado nos estudos de MALDI-TOF-MS foi determinada a massa molecular (MM) das subunidades da HbGp. Entretanto, ainda não era possível propor o valor exato da MM para a HbGp íntegra, pois a estequiometria deste oligôme
Publicado em: 2010
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2. Estudos bioquimicos e fisicos de hemoglobina extracelular de Glossoscolex paulistus (Oligochaeta)
Glossoscolex paulistus (Oligochaeta, Glossoscolecidae) is an earthworm which dwells in limestone regions in São Paulo State, and has a giant extracellular hemeprotein that occurs in the blood. This type of respiratory pigment is called erythrocruorin, and is examined with the aid of electronic microscopy as two hexagonal superimposed disks, each one having
Publicado em: 1988
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3. Extração, purificação e propriedades da polifenoloxidase da banana nanica Musa cavendishii, L
The polyphenoloxidase of banana "nanica" (Musa cavendishii L.) was extracted from the central part of half mature fruit by homogenising in a blender at room temperature with a buffer solution of 0.2 M phosphate (pH 7.0) containing 1% of insoluble PVP and 0.5% of Triton X-100. The crude extract was precipitated with 2 volumes of acetone, previously cooled dow
Publicado em: 1978
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4. Subunits of the alkaline phosphatase of Bacillus licheniformis: chemical, physicochemical, and dissociation studies.
The alkaline phosphatase (orthophosphoric monoester phosphydrolase, EC 3.1.3.1) of Bacillus licheniformis MC14 was studied in an attempt to determine the number of subunits contained in the 120,000-molecular-weight native enzyme. Two moles of arginine was liberated per mole of native enzyme by carboxypeptidases A and B in the presence of sodium dodecyl sulfa
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5. Disruption of Myxoviruses with Tween 20 and Isolation of Biologically Active Hemagglutinin and Neuraminidase Subunits
Myxoviruses were disrupted with Tween 20 at high pH, and the major surface antigens were separated in biologically active form. The neuraminidase had a sedimentation coefficient of 10.8S, and the hemagglutinin had a sedimentation coefficient of 8.1S. Electron microscopic examination of negatively stained preparations revealed structures identical in size and
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6. Structural Rearrangement and Subunit Composition of RNA from Released Soehner-Dmochowski Murine Sarcoma Virions
Two types of genomic, high-molecular-weight RNA species were found in Soehner-Dmochowski murine sarcoma virions released from virus-induced rat tumor cells grown in tissue culture. The type of RNA species observed depended on the length of exposure of the tumor cells to radioactive precursor. Early RNA of virions labeled up to 4 h with radioactive uridine ha
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7. Comparison of the Tryptophan Synthetase α Subunits of Several Species of Enterobacteriaceae
Creighton, T. E. (Stanford University, Stanford), D. R. Helinski, R. L. Somerville, and C. Yanofsky. Comparison of the tryptophan synthetase α subunits of several species of Enterobacteriaceae. J. Bacteriol. 91:1819–1826. 1966.—The tryptophan synthetase α subunits of Escherichia coli K-12, E. coli B, Shigella dysenteriae, Salmonella typhimurium, and Ae
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8. Solubilization of Envelopes of HVJ (Sendai virus) with Alkali-Emasol Treatment and Reassembly of Envelope Particles with Removal of the Detergent
The envelopes of HVJ (Sendai virus) virions were solubilized with alkali-Emasol treatment. The solubilized envelope subunit(s) associated with hemagglutination-inhibiting antibody blocking, neuraminidase, and low hemagglutinating (HA) activities had a sedimentation coefficient of 8.8S. Envelope fragment-like structures were assembled from the solubilized sub
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9. Total Reconstitution of Functionally Active 50S Ribosomal Subunits from Escherichia coli*
Total reconstitution of 50S subunits from E. coli was achieved by a two-step incubation procedure. In the first step, 23S RNA, 5S RNA, and the total proteins from 50S subunits were incubated for 20 min at 40° in the presence of 4 mM Mg++ and 400 mM NH4Cl. In the second step, the Mg++ concentration was raised to 20 mM and the incubation was performed for 90
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10. Structure of the Molybdoferredoxin Complex from Clostridium pasteurianum and Isolation of Its Subunits
Highly purified molybdoferredoxin, with a specific activity of 2.6 μmoles of acetylene reduced per min per mg of protein, was obtained from Clostridium pasteurianum. The protein at concentrations above 5 mg/ml exists in solution as a tetrameric complex with two subunits each of about 60,000 and 50,000 daltons. Two atoms of molybdenum are present per protein
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11. A cAMP-dependent protein kinase is present in differentiating Dictyostelium discoideum cells
We demonstrate the occurrence of a cAMP-dependent protein kinase in Dictyostelium discoideum cells at the terminal stage of differentiation. A cAMP-binding component was purified to homogeneity by affinity chromatography. This subunit inhibits the activity of purified catalytic subunit from beef heart protein kinase; the inhibition is reversed upon addition
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12. Production of a Noncovalently Bonded Pentamer of Immunoglobulin M: Relationship to J Chain
Human immunoglobulin M was reduced with concentrations of 2-mercaptoethylamine chosen so that approximately 40% of the immunoglobulin M was reduced to 7S subunits. Under these conditions, which selectively cleave intersubunit disulfides, J chain was released. The 7S subunits of immunoglobulin M so produced did not contain J chain. The high-molecular-weight i