Sucrose Binding Protein
Mostrando 1-12 de 297 artigos, teses e dissertações.
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1. Identificação de regiões no promotor do gene SBP2 (sucrose binding protein) de soja que conferem expressão espacial específica / Identification of regions on the soybean SBP2 (sucrose binding protein) promotor that confer tissue-specific expression
O promotor do gene SBP2 (sucrose binding protein) de soja é capaz de dirigir a expressão tecido vascular-específica de genes repórteres em plantas transgênicas de tabaco. Esta regulação se deve à presença de domínios cisregulatórios distais (CRD-A, posição -2000 a -700) presentes no promotor. Neste trabalho, a atividade tecido-específica de CRD
Publicado em: 2007
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2. Expressão heteróloga e caracterização bioquímica da proteína recombinante GmSBP2/S64 da soja (Glycine max) / Heterologous expression and biochemical characterization of GmSBP2/S64 recombinant protein from soybean (Glycine max)
The sucrose binding protein (SBP) belongs to the cupin family of proteins and is structurally related to vicilin-like storage proteins. In this investigation, SBP was expressed in E. coli and large amounts of the protein accumulated in the insoluble fraction as aggregated, denatured protein. The refolding of the purified protein proceeded with a progressive
Publicado em: 2006
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3. ImobilizaÃÃo de invertase em cinzas de carvÃo sinterizadas para hidrolise de sacarose: propriedades e aplicaÃÃo em biorreatores
Ceramic sinterized proceeding from coal fly ashes was used as a support for immobilization of invertase. The attainment of an insoluble support with chemical physical properties to bind invertase into sinterized coal fly ashes is an alternative to decrease environmental pollution with the storage and dissemination through thermal power plants. Of point view,
Publicado em: 2006
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4. Caracterização dos padrões de expressão de glucosiltransferases B e C, da proteina ligante de glucano B e de possiveis genes reguladores em genotipos distintos de Streptococcus mutans / Expression analysis of glucosyltransferases B and C, glucan-binding protein B and their putative regulatory genes in distinct genotypes of Streptococcus mutans
Streptococcus mutans, the main pathogen of dental caries, have the capacity to accumulate in the dental biofilm in the presence of sucrose, under highly acidic conditions that are responsible for teeth demineralization. The glucosyltransferases B (GtfB) and C (GtfC) produced by S. mutans are essential in this process, because catalyze the synthesis of water-
Publicado em: 2006
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5. The role of sucrose in dental biofilm formation and in the protein composition of biofilm matrix formed in situ / Papel da sacarose na formação do biofilme dental e na composição de proteinas da matriz do biofilme formado in situ
Dental caries is a biofilm-dependent oral disease, and fermentable dietary carbohydrates are the key environmental factors involved with its initiation and development. Some hypotheses based on the structure, composition and ion kinetic aspects of biofilm have been suggested to explain the cariogenicity of biofilm formed in the presence of sucrose. Among the
Publicado em: 2005
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6. Pulchellis: a patent vegetal toxin ribosome inactivating - type 2 RIP. in vitro and in vivo studies / Pulchellina: uma potente toxina vegetal inativadora de ribossomos - RIP tipo 2. estudos in vitro e in vivo
Pulchellin is a type 2 ribosome-inactivating protein (RIP) isolated from seeds of the Abrus pulchellus tenuiflorus plant. The DNA fiagment encoding Pulchellin A-chain (PAC) was cloned and inserted in pGEX-5X to express the recombinant pulchellin Achain (rPAC) as a fusion protein in Escherichin coli. The deduced amino acid sequence analyses of the rPAC presen
Publicado em: 2005
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7. Hemocianina de megalobulimulus ovatus : aspectos estruturais e funcionais
The hemocyanin from Megalobulimulus ovatus, gastropod of terrestrial habitat, was purified. It was shown the presence of at least two components, c* hemocyanin (71.5% of total hemocyanin), which dissociates in 1 M NaC 1 solution and 3 hemocyanin (28.5% of total hemocyanin) which remains intact in the same conditions. B hemocyanin was isolated by preparative
Publicado em: 1983
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8. A soybean sucrose binding protein independently mediates nonsaturable sucrose uptake in yeast.
Heterologous expression of a cDNA encoding a 62-kD soybean sucrose binding protein in yeast demonstrates that this protein, independent of other plant proteins, mediates sucrose uptake across the plasma membrane. Sucrose binding protein-mediated sucrose uptake is nonsaturable up to 30 mM sucrose, is specific for sucrose, and is relatively insensitive to trea
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9. A 62-kD sucrose binding protein is expressed and localized in tissues actively engaged in sucrose transport.
Sucrose transport from the apoplasm, across the plasma membrane, and into the symplast is critical for growth and development in most plant species. Phloem loading, the process of transporting sucrose against a concentration gradient into the phloem, is an essential first step in long-distance transport of sucrose and carbon partitioning. We report here that
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10. Redundancy in Periplasmic Binding Protein-Dependent Transport Systems for Trehalose, Sucrose, and Maltose in Sinorhizobium meliloti
We have identified a cluster of six genes involved in trehalose transport and utilization (thu) in Sinorhizobium meliloti. Four of these genes, thuE, -F, -G, and -K, were found to encode components of a binding protein-dependent trehalose/maltose/sucrose ABC transporter. Their deduced gene products comprise a trehalose/maltose-binding protein (ThuE), two int
American Society for Microbiology.
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11. Effects of antibodies against cell surface protein antigen PAc-glucosyltransferase fusion proteins on glucan synthesis and cell adhesion of Streptococcus mutans.
Cell surface protein antigen (PAc) and glucosyltransferases (GTFs) produced by Streptococcus mutans are considered to be major colonization factors of the organism, and the inhibition of these two factors is predicted to provide protection against dental caries. In this study, we have constructed fusion protein PAcA-GB, a fusion of the saliva-binding alanine
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12. Estrogen Receptors in Human Breast Cancer
Specific quantitative techniques have been used to measure the cytoplasmic estradiol-binding protein (EBP) in human mammary carcinoma tissue specimens. Sucrose gradient centrifugation reveals EBP to sediment at 8S and 4S. Variable quantities of non-specific estradiol binding occurs in the 4S region of the sucrose gradient necessitating controls to insure spe