Sucrose Phosphate Synthetase
Mostrando 1-12 de 24 artigos, teses e dissertações.
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1. Síntese da sacarose no amadurecimento da banana. Envolvimento da sacarose sintetase e sacarose fosfato sintetase / Sucrose synthesis during banana ripening: sucrose synthetase and sucrose phosphate synthetase involviment
A sacarose sintetase pode ser extraída de bananas pré-climatéricas cisteína (0,02M), em tampão tris-HCl pH 8,0 (0,05M), contendo EDTA (O,OlM), PVP (1%) e bissulfito de sódio (0,02M) na proporção de 1:4 (massa de banana/volume desolução f extratora). O seu isolamento pode ser efetuado por precipitação com sulfato de amônio, cromatografia por pene
Publicado em: 1989
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2. The Regulatory Properties of Purified Phaseolus aureus Sucrose Synthetase 1
Phaseolus aureus sucrose synthetase, purified to homogeneity, was assayed in the presence of a variety of biological compounds to test for possible regulatory effectors. The oxidized form of nicotinamide adenine dinucleotide phosphate, as well as indoleacetic acid, gibberellic acid, and pyrophosphate were found to activate the forward reaction (sucrose degra
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3. The Biosynthesis of Sucrose and Nucleoside Diphosphate Glucoses in Phaseolus aureus1
Sucrose-phosphate synthetase is detectable only in intact chloroplast preparations of Phaseolus aureus. In contrast, sucrose synthetase and uridine diphosphate glucose (UDP-glucose) pyrophosphorylase activities are low in extracts of photosynthetic tissues of P. aureus but are high in extracts of nonphotosynthetic tissues. Activities for ADP-, dTDP-, CDP-, a
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4. Subcellular Distribution of Gluconeogenetic Enzymes in Germinating Castor Bean Endosperm 1
The intracellular distribution of enzymes capable of catalyzing the reactions from oxaloacetate to sucrose in germinating castor bean endosperm has been studied by sucrose density gradient centrifugation. One set of glycolytic enzyme activities was detected in the plastids and another in the cytosol. The percentages of their activities in the plastids were l
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5. Enzymic Mechanism of Starch Breakdown in Germinating Rice Seeds: IV. De Novo Synthesis of Sucrose 6-Phosphate Synthetase in Scutellum 1
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6. Purification and Characterization of Sucrose Synthetase from the Shoot of Bamboo Leleba oldhami12
A 108-fold purification of the sucrose synthetase from the extract of the shoot of bamboo Lelaba oldhami was achieved by ammonium sulfate fractionation, calcium phosphate gel adsorption, and chromatographic separations on Sephadex G-100 and diethylaminoethyl-cellulose columns. Some properties of this enzyme, namely thermal and pH stabilities, stabilization b
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7. Localization of Glycerate Kinase and Some Enzymes for Sucrose Synthesis in C3 and C4 Plants 1
The localization of some key enzymes leading to sucrose synthesis in photosynthetic tissue of C3 and C4 species was investigated. These included UDP-glucose (UDPG) pyrophosphorylase, sucrose phosphate synthetase, and glycerate kinase. Whether glycerate kinase is localized exclusively in the chloroplast or partly outside the chloroplast could influence the fa
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8. Sucrose Translocation and Storage in the Sugar Beet 1
Several physiological processes were studied during sugar beet root development to determine the cellular events that are temporally correlated with sucrose storage. The prestorage stage was characterized by a marked increase in root fresh weight and a low sucrose to glucose ratio. Carbon derived from 14C-sucrose accumulation was partitioned into protein and
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9. Effect of Vanadium on Growth, Chemical Composition, and Metabolic Processes of Mature Sugar Beet (Beta vulgaris L.) Plants 1
As measured 7, 14, and 21 days after the application of 10−2 M vanadyl sulfate solution to the foliage of 4.5-month-old sugar beet plants, significantly less growth of the leaves and an increase in the sucrose content of the storage root resulted. Accompanying these alterations were a higher rate of carbon dioxide fixation, a lower rate of respiration, and
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10. Photosynthate Partitioning in Soybean Leaves at Two Irradiance Levels: Comparative Responses of Acclimated and Unacclimated Leaves
High irradiance-acclimated soybean leaves had the same CO2 exchange rates, but lower starch accumulation rates and correspondingly higher translocation rates than unacclimated leaves. Increased translocation rates were associated with increased sucrose phosphate synthetase (EC 2.4.1.14) activity. Foliar sucrose levels and adenosine diphosphate-glucose pyroph
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11. Isolation and Characterization of a Glucosamine-Requiring Mutant of Escherichia coli K-12 Defective in Glucosamine-6-Phosphate Synthetase
A mutant was isolated from Escherichia coli K-12 which requires glucosamine or N-acetylglucosamine for growth. Depriving the mutant of glucosamine resulted in a rapid loss of viability of the cells, followed by a decrease in the turbidity of the culture. When the mutant cells were resuspended in broth media containing 10% sucrose, the rod-shaped cells became
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12. Proteolytically induced changes in the molecular form of the carbamyl phosphate synthetase-uracil-aspartate transcarbamylase complex coded for by the URA2 locus in Saccharomyces cerevisiae.
When a uracil-auxotrophic yeast strain is grown under uracil-limiting conditions, the aspartate transcarbamylase activity found in crude extracts shows a variation in sensitivity to feedback inhibition by uridine 5'-triphosphate. In this study we correlated this variation with changes in the molecular form of the carbamyl phosphate synthetase-uracil-aspartat