Sucrose Synthetase
Mostrando 1-12 de 91 artigos, teses e dissertações.
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1. Metabolite and light regulation of metabolism in plants: lessons from the study of a single biochemical pathway
We are using molecular, biochemical, and genetic approaches to study the structural and regulatory genes controlling the assimilation of inorganic nitrogen into the amino acids glutamine, glutamate, aspartate and asparagine. These amino acids serve as the principal nitrogen-transport amino acids in most crop and higher plants including Arabidopsis thaliana.
Brazilian Journal of Medical and Biological Research. Publicado em: 2001-05
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2. Síntese da sacarose no amadurecimento da banana. Envolvimento da sacarose sintetase e sacarose fosfato sintetase / Sucrose synthesis during banana ripening: sucrose synthetase and sucrose phosphate synthetase involviment
A sacarose sintetase pode ser extraída de bananas pré-climatéricas cisteína (0,02M), em tampão tris-HCl pH 8,0 (0,05M), contendo EDTA (O,OlM), PVP (1%) e bissulfito de sódio (0,02M) na proporção de 1:4 (massa de banana/volume desolução f extratora). O seu isolamento pode ser efetuado por precipitação com sulfato de amônio, cromatografia por pene
Publicado em: 1989
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3. A cDNA clone from Zea mays endosperm sucrose synthetase mRNA.
A cDNA clone for maize endosperm sucrose synthetase of 62o nucleotide pairs length was obtained by cloning double stranded DNA obtained from the total maize endosperm poly(A) RNA in pBR322, and identifying the appropriate clone by hybrid-promoter translation. In Southern blotting to genomic BamHI-digested DNA, a single band only of approximately 20 Kb lights
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4. The Biosynthesis of Sucrose and Nucleoside Diphosphate Glucoses in Phaseolus aureus1
Sucrose-phosphate synthetase is detectable only in intact chloroplast preparations of Phaseolus aureus. In contrast, sucrose synthetase and uridine diphosphate glucose (UDP-glucose) pyrophosphorylase activities are low in extracts of photosynthetic tissues of P. aureus but are high in extracts of nonphotosynthetic tissues. Activities for ADP-, dTDP-, CDP-, a
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5. Regulation of β-Glucan Synthetase Activity by Auxin in Pea Stem Tissue: I. Kinetic Aspects 1
Treatment of pea stem segments with indoleacetic acid (IAA) causes within 1 hour a 2- to 4-fold increase in activity of particulate uridine diphosphoglucose-dependent β-glucan synthetase obtainable from the tissue. The IAA effect is observable in tissue from all parts of the elongation zone of the pea stem, and also in older tissue that is not capable of a
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6. Subcellular Distribution of Gluconeogenetic Enzymes in Germinating Castor Bean Endosperm 1
The intracellular distribution of enzymes capable of catalyzing the reactions from oxaloacetate to sucrose in germinating castor bean endosperm has been studied by sucrose density gradient centrifugation. One set of glycolytic enzyme activities was detected in the plastids and another in the cytosol. The percentages of their activities in the plastids were l
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7. Potato Sucrose Synthetase: Purification, Properties, and Changes in Activity Associated With Maturation
Sucrose synthetase activity is high in young potato tubers but decreases markedly during maturation. The activity decreases rapidly after the tubers are harvested and remains low regardless of storage temperature. This enzyme was purified 34-fold from freshly harvested immature potatoes. It catalyzes both cleavage and synthesis of sucrose but the 2 activitie
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8. Sucrose Translocation and Storage in the Sugar Beet 1
Several physiological processes were studied during sugar beet root development to determine the cellular events that are temporally correlated with sucrose storage. The prestorage stage was characterized by a marked increase in root fresh weight and a low sucrose to glucose ratio. Carbon derived from 14C-sucrose accumulation was partitioned into protein and
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9. Enzymes of Carbohydrate Metabolism in the Developing Rice Grain
The levels of reducing and nonreducing sugars, starch, soluble protein, and selected enzymes involved in the metabolism of sucrose, glucose-1-P, and glucose nucleotides were assayed in dehulled developing rice grains (Oryza sativa L. line IR1541-76-3) during the first 3 weeks after flowering. The level of reducing sugars in the grain was highest 5 to 6 days
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10. Purification and Characterization of Sucrose Synthetase from the Shoot of Bamboo Leleba oldhami12
A 108-fold purification of the sucrose synthetase from the extract of the shoot of bamboo Lelaba oldhami was achieved by ammonium sulfate fractionation, calcium phosphate gel adsorption, and chromatographic separations on Sephadex G-100 and diethylaminoethyl-cellulose columns. Some properties of this enzyme, namely thermal and pH stabilities, stabilization b
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11. Regulation of synthesis of hepatic fatty acid synthetase: binding of fatty acid synthetase antibodies to polysomes.
Mammalian fatty acid synthetase was shown to be composed of two peptides, molecular weight 240,000, after dissociation with sodium dodecyl sulfate. Rat liver polysomes that synthesize fatty acid synthetase were identified by sucrose gradient analysis of polysomes that had been reacted with 125I-labeled antibody against fatty acid synthetase. The binding of 1
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12. Localization of Glycerate Kinase and Some Enzymes for Sucrose Synthesis in C3 and C4 Plants 1
The localization of some key enzymes leading to sucrose synthesis in photosynthetic tissue of C3 and C4 species was investigated. These included UDP-glucose (UDPG) pyrophosphorylase, sucrose phosphate synthetase, and glycerate kinase. Whether glycerate kinase is localized exclusively in the chloroplast or partly outside the chloroplast could influence the fa