Topology Change
Mostrando 1-12 de 60 artigos, teses e dissertações.
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1. Effect of transcription of yeast chromatin on DNA topology in vivo.
Coding regions of transcribed and non-transcribed genes typically differ in chromatin structure. However, it is not known what kind of alterations in nucleosome or chromatin structure these differences reflect. To determine whether changes in nucleosome topology accompany transcription, we introduced into yeast a multicopy plasmid bearing the gene coding for
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2. A polytopic membrane protein displays a reversible topology dependent on membrane lipid composition
To address the role of phospholipids in the topological organization of polytopic membrane proteins, the function and assembly of lactose permease (LacY) was studied in mutants of Escherichia coli lacking phosphatidylethanolamine (PE). PE is required for the proper conformation and active transport function of LacY. The N-terminal half of LacY assembled in P
Oxford University Press.
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3. Graphene to graphite: electronic changes within DFT calculations
Calculations based on the first-principles pseudopotential plane-wave method and density-functional theory are performed to investigate the electronic properties of graphene, bilayer graphene, multilayer graphene, and graphite. From an analysis of the electronic band structure close to the Fermi level, we have quantified the gradual change in the Fermi surfa
Brazilian Journal of Physics. Publicado em: 2009-12
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4. The protein encoded by the proto-oncogene DEK changes the topology of chromatin and reduces the efficiency of DNA replication in a chromatin-specific manner
The structure of chromatin regulates the genetic activity of the underlying DNA sequence. We report here that the protein encoded by the proto-oncogene DEK, which is involved in acute myelogenous leukemia, induces alterations of the superhelical density of DNA in chromatin. The change in topology is observed with chromatin but not with naked DNA and does not
Cold Spring Harbor Laboratory Press.
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5. Interaction of the histone (H3-H4)2 tetramer of the nucleosome with positively supercoiled DNA minicircles: Potential flipping of the protein from a left- to a right-handed superhelical form.
We have studied the ability of the histone (H3-H4)2 tetramer, the central part of the nucleosome of eukaryotic chromatin, to form particles on DNA minicircles of negative and positive superhelicities, and the effect of relaxing these particles with topoisomerase I. The results show that even modest positive torsional stress from the DNA, and in particular th
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6. DNA topoisomerase I controls the kinetics of promoter activation and DNA topology in Saccharomyces cerevisiae.
Inactivation of the nonessential TOP1 gene, which codes for Saccharomyces cerevisiae DNA topoisomerase I, affects the rate of transcription starting at the ADH2 promoter. For both the chromosomal gene and the plasmid-borne promoter, mRNA accumulation is kinetically favored in the mutant relative to a wild-type isogenic strain. The addition of ethanol causes
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7. Projeto de atuadores piezelétricos flextensionais usando o método de otimização topológica. / Design of flextensional piezoelectric actuator using the topology optimization method.
Flextensional Piezoelectric Actuators consist of a flexible structure actuated by piezoelectric ceramics (or a stack of piezoceramics). The flexible structure connected to the piezoceramic must generate displacements and forces in different specified points of the domain, according to a specific direction. These actuators are applied to precision mechanic ap
Publicado em: 2003
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8. Endogenous Fluctuations of DNA Topology in the Chloroplast of Chlamydomonas reinhardtii
DNA supercoiling in the chloroplast of the unicellular green alga Chlamydomonas reinhardtii was found to change with a diurnal rhythm in cells growing in alternating 12-h dark–12-h light periods. Highest and lowest DNA superhelicities occurred at the beginning and towards the end of the 12-h light periods, respectively. The fluctuations in DNA supercoiling
American Society for Microbiology.
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9. The Yersinia enterocolitica pYV Virulence Plasmid Contains Multiple Intrinsic DNA Bends Which Melt at 37°C
Temperature has a pleiotropic effect on Yersinia enterocolitica gene expression. Temperature-dependent phenotypes include the switching between two type III protein secretion systems, flagellum biosynthesis (≤30°C) and virulence plasmid-encoded Yop secretion (37°C). The mechanism by which temperature exerts this change in genetic programming is unclear;
American Society for Microbiology.
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10. Shapes and textures of ferromagnetic liquid droplets
Theoretical calculations, computer simulations and experiments indicate the possible existence of a ferromagnetic liquid state. Should such a state exist, demagnetization effects would force a nontrivial magnetization texture governed by the shape of the liquid droplet. Since liquid droplets are deformable, the droplet shape couples to the magnetization text
Braz. J. Phys.. Publicado em: 2001-09
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11. Effects of histone acetylation on chromatin topology in vivo.
Recently a model for eukaryotic transcriptional activation has been proposed in which histone hyperacetylation causes release of nucleosomal supercoils, and this unconstrained tension in turn stimulates transcription (V. G. Norton, B. S. Imai, P. Yau, and E. M. Bradbury, Cell 57:449-457, 1989; V. G. Norton, K. W. Marvin, P. Yau, and E. M. Bradbury, J. Biol.
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12. The architecture of the human Rad54–DNA complex provides evidence for protein translocation along DNA
Proper maintenance and duplication of the genome require accurate recombination between homologous DNA molecules. In eukaryotic cells, the Rad51 protein mediates pairing between homologous DNA molecules. This reaction is assisted by the Rad54 protein. To gain insight into how Rad54 functions, we studied the interaction of the human Rad54 (hRad54) protein wit
The National Academy of Sciences.