Uncoupling Mitochondrial Protein
Mostrando 1-12 de 60 artigos, teses e dissertações.
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1. Evaluation of the tissue and circulating renin-angiotensin system in offspring of dams that received salt overload or restriction during pregnancy and lactation / Avaliação do sistema renina - angiotensina tecidual e circulante em prole de ratas que receberam sobrecarga ou restrição de sal na dieta durante a gestação e amamentação
Epidemiologic studies reported that insults during the intrauterine life have been associated with many abnormalites such low birth weigth, type 2 diabetes, hypertension and obesity in adulthood[1]. Low birth weight and obesity in adulthood were observed in offspring of undernourished dams. In addition, a high expression of genes related with lipid metabolis
Publicado em: 2006
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2. Atividade respiratória e metabolismo antioxidativo em raízes de plântulas de milho (Zea mays L.) submetidas ao estresse salino / Respiration activity and antioxidative metabolism in roots of maize (Zea mays L.) seedlings submitted to salt stress
Tolerance to salt stress was studied in maize seedlings (Zea mays L.) cultivars AGN 3150, BR 106, BR 201, BR 206 and SHS 4040, grown in Hoaglands nutrient solution. Treatments with NaCl 50 and 100 mM led to a decrease in the biomass production and to an increase in the relative electrolyte leakage in the roots and shoots in all cultivars. However, the larges
Publicado em: 2006
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3. Respiração e atividade de enzimas do metabolismo antioxidativo em raízes de plântulas de milho (Zea mays L.) submetidas ao estresse por alumínio / Respiration and activity of antioxidant enzymes in roots of corn (Zea mays L.) seedlings submitted to aluminum
Os efeitos do alumínio sobre o crescimento, a respiração e as atividades de algumas enzimas envolvidas na eliminação de espécies reativas de oxigênio foram avaliadas em plântulas de duas cultivares de milho, uma sensível (BR 106) e outra tolerante ao alumínio (BR 206), cultivadas em solução nutritiva, pH 4,0. O alumínio, nas concentrações de 5
Publicado em: 2006
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4. Respiration, oxidative phosphorylation, and uncoupling protein in Candida albicans
The respiration, membrane potential (Dy), and oxidative phosphorylation of mitochondria in situ were determined in spheroplasts obtained from Candida albicans control strain ATCC 90028 by lyticase treatment. Mitochondria in situ were able to phosphorylate externally added ADP (200 µM) in the presence of 0.05% BSA. Mitochondria in si
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-10
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5. Hypercaloric cafeteria-like diet induced UCP3 gene expression in skeletal muscle is impaired by hypothyroidism
The uncoupling protein UCP3 belongs to a family of mitochondrial carriers located in the inner mitochondrial membrane of certain cell types. It is expressed almost exclusively at high levels in skeletal muscle and its physiological role has not been fully determined in this tissue. In the present study we have addressed the possible interaction between a hyp
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-06
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6. Identificação e caracterização de genes expressos em resposta ao estresse por baixa temperatura em cana-de-açucar
Environmental stresses represent one of the main factors limiting crop productivity, being very important studies that aim to analyze, at a molecular level, the effects caused by these stresses. In the present project, we analyzed the sugarcane gene expression profiles in response to low temperature. Initially, high-density filter arrays containing 1536 ESTs
Publicado em: 2004
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7. Proteina desacopladora de plantas : efeito do estresse oxidativo na sua expressão e atividade e reconstituição funcional da isoforma de Arabidopsis thaliana
The present work is divided in two parts: the functional reconstitution of the Arabidopsis thaZiana uncoupling protein 1 (AtPUMPI) and the expression and activity of the uncoupling protein under several abiotic stresses. AtPUMPI expression in Saccharomyces cerevisae and Escherichia coZi allowed us to confirm that this gene codes for a true uncoupling protein
Publicado em: 2003
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8. Complete nucleotide and derived amino acid sequence of cDNA encoding the mitochondrial uncoupling protein of rat brown adipose tissue: lack of a mitochondrial targeting presequence.
A cDNA clone spanning the entire amino acid sequence of the nuclear-encoded uncoupling protein of rat brown adipose tissue mitochondria has been isolated and sequenced. With the exception of the N-terminal methionine the deduced N-terminus of the newly synthesized uncoupling protein is identical to the N-terminal 30 amino acids of the native uncoupling prote
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9. Stable expression of functional mitochondrial uncoupling protein in Chinese hamster ovary cells.
The mitochondrial uncoupling protein (UCP) is a membranous proton carrier exclusively synthesized in brown adipocytes. The cDNA for the rat UCP was placed in an expression vector and transfected into mammalian cells. Its expression was tested in transiently transfected CHO cells. In these cells the UCP was detected in mitochondria by using antibodies. Perman
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10. Assessment of mitochondrial energy coupling in vivo by 13C/31P NMR
The recently cloned uncoupling protein homolog UCP3 is expressed primarily in muscle and therefore may play a significant role in the regulation of energy expenditure and body weight. However, investigation into the regulation of uncoupling protein has been hampered by the inability to assess its activity in vivo. In this report, we demonstrate the use of a
National Academy of Sciences.
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11. The mitochondrial uncoupling protein gene in brown fat: correlation between DNase I hypersensitivity and expression in transgenic mice.
The mitochondrial uncoupling protein gene is rapidly induced in mouse brown fat following cold exposure. To identify cis-regulatory elements, approximately 50 kb of chromatin surrounding the uncoupling protein gene was examined for its hypersensitivity to DNase I. Seven DNase I-hypersensitive sites were identified in the 5'-flanking DNA, and one site was ide
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12. Threonine-deficient diets induced changes in hepatic bioenergetics
Diets deficient in an indispensable amino acid are known to suppress food intake in rats. Few studies were focused at understanding how amino acid-deficient diets may elicit biochemical changes at the mitochondrial level. The goal of this study was to evaluate mitochondrial function in rats fed diets with 0.00, 0.18, 0.36, and 0.88% threonine (Thr) (set at 0
American Physiological Society.